PTR39_ARATH
ID PTR39_ARATH Reviewed; 558 AA.
AC Q9M175; Q8GYF8;
DT 02-NOV-2010, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 112.
DE RecName: Full=Protein NRT1/ PTR FAMILY 2.3;
DE Short=AtNPF2.3;
DE AltName: Full=Probable nitrate excretion transporter 3;
DE AltName: Full=Protein NAXT1-like 2;
GN Name=NPF2.3; OrderedLocusNames=At3g45680; ORFNames=T6D9.10;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130713; DOI=10.1038/35048706;
RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL Nature 408:820-822(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=11910074; DOI=10.1126/science.1071006;
RA Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T.,
RA Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y.,
RA Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K.,
RA Shinagawa A., Shinozaki K.;
RT "Functional annotation of a full-length Arabidopsis cDNA collection.";
RL Science 296:141-145(2002).
RN [4]
RP INDUCTION BY NEMATODES.
RX PubMed=16478044; DOI=10.1094/mpmi-18-1247;
RA Hammes U.Z., Schachtman D.P., Berg R.H., Nielsen E., Koch W.,
RA McIntyre L.M., Taylor C.G.;
RT "Nematode-induced changes of transporter gene expression in Arabidopsis
RT roots.";
RL Mol. Plant Microbe Interact. 18:1247-1257(2005).
RN [5]
RP TISSUE SPECIFICITY, AND GENE FAMILY.
RX PubMed=17481610; DOI=10.1016/j.febslet.2007.04.047;
RA Tsay Y.F., Chiu C.C., Tsai C.B., Ho C.H., Hsu P.K.;
RT "Nitrate transporters and peptide transporters.";
RL FEBS Lett. 581:2290-2300(2007).
RN [6]
RP IDENTIFICATION, AND TISSUE SPECIFICITY.
RX PubMed=17993627; DOI=10.1105/tpc.106.048173;
RA Segonzac C., Boyer J.C., Ipotesi E., Szponarski W., Tillard P.,
RA Touraine B., Sommerer N., Rossignol M., Gibrat R.;
RT "Nitrate efflux at the root plasma membrane: identification of an
RT Arabidopsis excretion transporter.";
RL Plant Cell 19:3760-3777(2007).
RN [7]
RP GENE FAMILY.
RX PubMed=20501909; DOI=10.1105/tpc.110.075242;
RA Li J.Y., Fu Y.L., Pike S.M., Bao J., Tian W., Zhang Y., Chen C.Z.,
RA Zhang Y., Li H.M., Huang J., Li L.G., Schroeder J.I., Gassmann W.,
RA Gong J.M.;
RT "The Arabidopsis nitrate transporter NRT1.8 functions in nitrate removal
RT from the xylem sap and mediates cadmium tolerance.";
RL Plant Cell 22:1633-1646(2010).
RN [8]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=24055139; DOI=10.1016/j.tplants.2013.08.008;
RA Leran S., Varala K., Boyer J.C., Chiurazzi M., Crawford N.,
RA Daniel-Vedele F., David L., Dickstein R., Fernandez E., Forde B.,
RA Gassmann W., Geiger D., Gojon A., Gong J.M., Halkier B.A., Harris J.M.,
RA Hedrich R., Limami A.M., Rentsch D., Seo M., Tsay Y.F., Zhang M.,
RA Coruzzi G., Lacombe B.;
RT "A unified nomenclature of NITRATE TRANSPORTER 1/PEPTIDE TRANSPORTER family
RT members in plants.";
RL Trends Plant Sci. 19:5-9(2014).
CC -!- FUNCTION: Transporter involved in a passive nitrate efflux.
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000250}; Multi-pass membrane
CC protein {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Expressed in flowers, siliques and root epidermis
CC or cortex. Detected in shoots. {ECO:0000269|PubMed:17481610,
CC ECO:0000269|PubMed:17993627}.
CC -!- INDUCTION: Up-regulated upon nematode infection.
CC {ECO:0000269|PubMed:16478044}.
CC -!- SIMILARITY: Belongs to the major facilitator superfamily. Proton-
CC dependent oligopeptide transporter (POT/PTR) (TC 2.A.17) family.
CC {ECO:0000305}.
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DR EMBL; AL157735; CAB75781.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE78059.1; -; Genomic_DNA.
DR EMBL; AK117660; BAC42313.1; -; mRNA.
DR PIR; T47508; T47508.
DR RefSeq; NP_190154.1; NM_114437.5.
DR AlphaFoldDB; Q9M175; -.
DR SMR; Q9M175; -.
DR STRING; 3702.AT3G45680.1; -.
DR PaxDb; Q9M175; -.
DR PRIDE; Q9M175; -.
DR ProteomicsDB; 226238; -.
DR EnsemblPlants; AT3G45680.1; AT3G45680.1; AT3G45680.
DR GeneID; 823710; -.
DR Gramene; AT3G45680.1; AT3G45680.1; AT3G45680.
DR KEGG; ath:AT3G45680; -.
DR Araport; AT3G45680; -.
DR TAIR; locus:2102659; AT3G45680.
DR eggNOG; KOG1237; Eukaryota.
DR HOGENOM; CLU_009313_4_2_1; -.
DR InParanoid; Q9M175; -.
DR OMA; TSFGWVM; -.
DR OrthoDB; 365203at2759; -.
DR PhylomeDB; Q9M175; -.
DR PRO; PR:Q9M175; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q9M175; baseline and differential.
DR Genevisible; Q9M175; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IDA:TAIR.
DR GO; GO:0015112; F:nitrate transmembrane transporter activity; IDA:TAIR.
DR GO; GO:0071472; P:cellular response to salt stress; IMP:TAIR.
DR GO; GO:0042128; P:nitrate assimilation; IEA:UniProtKB-KW.
DR GO; GO:0015706; P:nitrate transmembrane transport; IMP:TAIR.
DR GO; GO:0006857; P:oligopeptide transport; IEA:InterPro.
DR GO; GO:0009624; P:response to nematode; HEP:TAIR.
DR Gene3D; 1.20.1250.20; -; 1.
DR InterPro; IPR036259; MFS_trans_sf.
DR InterPro; IPR000109; POT_fam.
DR InterPro; IPR018456; PTR2_symporter_CS.
DR PANTHER; PTHR11654; PTHR11654; 1.
DR Pfam; PF00854; PTR2; 1.
DR SUPFAM; SSF103473; SSF103473; 1.
DR PROSITE; PS01022; PTR2_1; 1.
PE 2: Evidence at transcript level;
KW Membrane; Nitrate assimilation; Reference proteome; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..558
FT /note="Protein NRT1/ PTR FAMILY 2.3"
FT /id="PRO_0000399973"
FT TRANSMEM 33..53
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 69..89
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 92..112
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 128..148
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 176..196
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 203..223
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 329..349
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 371..391
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 403..423
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 439..459
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 478..498
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 517..537
FT /note="Helical"
FT /evidence="ECO:0000255"
FT CONFLICT 76
FT /note="M -> V (in Ref. 3; BAC42313)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 558 AA; 61250 MW; 49BBCF7CECDC6762 CRC64;
MASLVSGDKE AQISGDPGSK RGGWITFPFM LATLLGLSVT SFGWVMNLIV FLIEEFNIKS
IAAAQISNVA NGCLSMLPVV AAILADSFFG NIPVIAASSF ISLLGIVLLT LIASLDYLRP
RPCEAGSVLC TPPSKLHLGI LYTALALVTT GAGGTRFTMA SAGANQYEKP KEQGSFFNWY
FLTLYAGAIT GATAIVYIQD NASWKLGFGL CAAANLISFI VFVSGKRYYK HDKPMGSPFT
SLIRVVVSAT VKRKAVISCN EEDYHHYGLE KEVKTSAAMP SKSFRFLNRA ALMTKDDLNQ
KEGSVNNIWR LCSVQEVEDF KAILRVFPLW LSIIFVSTPM VMQTSLIVLQ ALVTDRGLGP
NFKVPAGSLQ VIIIITACIV IIMNNWLVFP MYKKLTHKLL TPLQKVGIGQ VLTILSMALS
AVVEAKRLKT VENGHPMSVL WLFPPLVIVG IGEAFQFPAN IELFYGEFPE SLRNTATSLT
SVVIGISFYL STALIDLIQR TTAWLPNDIN HGRVDNVYWL LVIGGILNFG YFLVCSWVYK
YRNLKDNDQE QDPKDGTM
