PTR42_ARATH
ID PTR42_ARATH Reviewed; 560 AA.
AC Q9M172; F4J6Q7;
DT 02-NOV-2010, integrated into UniProtKB/Swiss-Prot.
DT 25-JAN-2012, sequence version 2.
DT 03-AUG-2022, entry version 110.
DE RecName: Full=Protein NRT1/ PTR FAMILY 2.5;
DE Short=AtNPF2.5;
DE AltName: Full=Probable nitrate excretion transporter 6;
DE AltName: Full=Protein NAXT1-like 5;
GN Name=NPF2.5; OrderedLocusNames=At3g45710; ORFNames=T6D9.40;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130713; DOI=10.1038/35048706;
RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL Nature 408:820-822(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP TISSUE SPECIFICITY, AND GENE FAMILY.
RX PubMed=17481610; DOI=10.1016/j.febslet.2007.04.047;
RA Tsay Y.F., Chiu C.C., Tsai C.B., Ho C.H., Hsu P.K.;
RT "Nitrate transporters and peptide transporters.";
RL FEBS Lett. 581:2290-2300(2007).
RN [4]
RP IDENTIFICATION, AND TISSUE SPECIFICITY.
RX PubMed=17993627; DOI=10.1105/tpc.106.048173;
RA Segonzac C., Boyer J.C., Ipotesi E., Szponarski W., Tillard P.,
RA Touraine B., Sommerer N., Rossignol M., Gibrat R.;
RT "Nitrate efflux at the root plasma membrane: identification of an
RT Arabidopsis excretion transporter.";
RL Plant Cell 19:3760-3777(2007).
RN [5]
RP GENE FAMILY.
RX PubMed=20501909; DOI=10.1105/tpc.110.075242;
RA Li J.Y., Fu Y.L., Pike S.M., Bao J., Tian W., Zhang Y., Chen C.Z.,
RA Zhang Y., Li H.M., Huang J., Li L.G., Schroeder J.I., Gassmann W.,
RA Gong J.M.;
RT "The Arabidopsis nitrate transporter NRT1.8 functions in nitrate removal
RT from the xylem sap and mediates cadmium tolerance.";
RL Plant Cell 22:1633-1646(2010).
RN [6]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=24055139; DOI=10.1016/j.tplants.2013.08.008;
RA Leran S., Varala K., Boyer J.C., Chiurazzi M., Crawford N.,
RA Daniel-Vedele F., David L., Dickstein R., Fernandez E., Forde B.,
RA Gassmann W., Geiger D., Gojon A., Gong J.M., Halkier B.A., Harris J.M.,
RA Hedrich R., Limami A.M., Rentsch D., Seo M., Tsay Y.F., Zhang M.,
RA Coruzzi G., Lacombe B.;
RT "A unified nomenclature of NITRATE TRANSPORTER 1/PEPTIDE TRANSPORTER family
RT members in plants.";
RL Trends Plant Sci. 19:5-9(2014).
CC -!- FUNCTION: Transporter involved in a passive nitrate efflux.
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000250}; Multi-pass membrane
CC protein {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Expressed in the root epidermis or cortex.
CC {ECO:0000269|PubMed:17481610, ECO:0000269|PubMed:17993627}.
CC -!- SIMILARITY: Belongs to the major facilitator superfamily. Proton-
CC dependent oligopeptide transporter (POT/PTR) (TC 2.A.17) family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAB75784.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AL157735; CAB75784.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002686; AEE78062.1; -; Genomic_DNA.
DR PIR; T47511; T47511.
DR RefSeq; NP_190157.2; NM_114440.4.
DR AlphaFoldDB; Q9M172; -.
DR SMR; Q9M172; -.
DR STRING; 3702.AT3G45710.1; -.
DR PaxDb; Q9M172; -.
DR PRIDE; Q9M172; -.
DR ProteomicsDB; 224835; -.
DR EnsemblPlants; AT3G45710.1; AT3G45710.1; AT3G45710.
DR GeneID; 823713; -.
DR Gramene; AT3G45710.1; AT3G45710.1; AT3G45710.
DR KEGG; ath:AT3G45710; -.
DR Araport; AT3G45710; -.
DR TAIR; locus:2102689; AT3G45710.
DR eggNOG; KOG1237; Eukaryota.
DR HOGENOM; CLU_009313_4_2_1; -.
DR InParanoid; Q9M172; -.
DR OMA; MAFYYME; -.
DR OrthoDB; 365203at2759; -.
DR PRO; PR:Q9M172; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q9M172; baseline and differential.
DR Genevisible; Q9M172; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0015108; F:chloride transmembrane transporter activity; IDA:TAIR.
DR GO; GO:1902075; P:cellular response to salt; IEP:TAIR.
DR GO; GO:0006821; P:chloride transport; IDA:TAIR.
DR GO; GO:0042128; P:nitrate assimilation; IEA:UniProtKB-KW.
DR GO; GO:0006857; P:oligopeptide transport; IEA:InterPro.
DR Gene3D; 1.20.1250.20; -; 1.
DR InterPro; IPR036259; MFS_trans_sf.
DR InterPro; IPR000109; POT_fam.
DR InterPro; IPR018456; PTR2_symporter_CS.
DR PANTHER; PTHR11654; PTHR11654; 1.
DR Pfam; PF00854; PTR2; 1.
DR SUPFAM; SSF103473; SSF103473; 1.
DR PROSITE; PS01022; PTR2_1; 1.
PE 2: Evidence at transcript level;
KW Membrane; Nitrate assimilation; Reference proteome; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..560
FT /note="Protein NRT1/ PTR FAMILY 2.5"
FT /id="PRO_0000399976"
FT TRANSMEM 34..54
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 77..97
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 101..121
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 141..161
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 177..197
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 207..227
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 323..343
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 372..392
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 404..424
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 441..461
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 480..500
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 520..540
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 1..20
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 560 AA; 61643 MW; 38130AF0A8402DF0 CRC64;
MADSKSGDTE VAHRSSDPSE KRGGWITLPF MLVTLLGMSI TSFGWGMNLI VFLIEEFHIK
NIAAAQISNV VNGVVNMLPV VAAILADSFF GNIPVISTST FISLAGTSLL TLITSLNYLM
PRPCETGSIL CQSPSKLQLG ILYVALALVI IGSAGTRFTL AAAGANQYKK PKEQGRFFNW
FFLALYIGAI TGTTAIVYTQ DNASWKLGFG LCAVANLISF IVFIAGVRFY KHDKPLGSPY
TSLIRVLVAA TMKRKAVISS KDEDYHQYGL GKEAKTYTTM PSKSFRFLNR AALKNKEDLN
TSGDSSNNMW RLCSVQEVED FKAILRLVPL WAAVMFLSTP VAVQMSMTVL QALVMDRKLS
PHFEVSAGSL QVIVLVFGCV FIMLNNWIIY PMYQKLIGKP LTPLQQVGIG HVFTILSMAI
SAVVEAKRLK TVENGGHPMS VLWLVPALVM VGIGEAFHFP ANVAVFYGEF PESLKNTATS
LTSVVIGISF YLSTAVIDVI QRTTSWLPND INHGRVDNVY WVVVIGGVLN LGYFLVCSWF
YKYRNLKDDD HEQDPKDVKT
