PTR47_ARATH
ID PTR47_ARATH Reviewed; 589 AA.
AC Q8GXN2; Q9SVS9;
DT 02-NOV-2010, integrated into UniProtKB/Swiss-Prot.
DT 02-NOV-2010, sequence version 2.
DT 03-AUG-2022, entry version 94.
DE RecName: Full=Protein NRT1/ PTR FAMILY 7.2;
DE Short=AtNPF7.2;
DE AltName: Full=Nitrate transporter 1.8;
GN Name=NPF7.2; Synonyms=NRT1.8; OrderedLocusNames=At4g21680;
GN ORFNames=F17L22.140;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617198; DOI=10.1038/47134;
RA Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA Martienssen R., McCombie W.R.;
RT "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL Nature 402:769-777(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=11910074; DOI=10.1126/science.1071006;
RA Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T.,
RA Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y.,
RA Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K.,
RA Shinagawa A., Shinozaki K.;
RT "Functional annotation of a full-length Arabidopsis cDNA collection.";
RL Science 296:141-145(2002).
RN [4]
RP TISSUE SPECIFICITY, AND GENE FAMILY.
RX PubMed=17481610; DOI=10.1016/j.febslet.2007.04.047;
RA Tsay Y.F., Chiu C.C., Tsai C.B., Ho C.H., Hsu P.K.;
RT "Nitrate transporters and peptide transporters.";
RL FEBS Lett. 581:2290-2300(2007).
RN [5]
RP FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INDUCTION BY CADMIUM
RP AND NITRATE, DISRUPTION PHENOTYPE, AND GENE FAMILY.
RX PubMed=20501909; DOI=10.1105/tpc.110.075242;
RA Li J.Y., Fu Y.L., Pike S.M., Bao J., Tian W., Zhang Y., Chen C.Z.,
RA Zhang Y., Li H.M., Huang J., Li L.G., Schroeder J.I., Gassmann W.,
RA Gong J.M.;
RT "The Arabidopsis nitrate transporter NRT1.8 functions in nitrate removal
RT from the xylem sap and mediates cadmium tolerance.";
RL Plant Cell 22:1633-1646(2010).
RN [6]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=24055139; DOI=10.1016/j.tplants.2013.08.008;
RA Leran S., Varala K., Boyer J.C., Chiurazzi M., Crawford N.,
RA Daniel-Vedele F., David L., Dickstein R., Fernandez E., Forde B.,
RA Gassmann W., Geiger D., Gojon A., Gong J.M., Halkier B.A., Harris J.M.,
RA Hedrich R., Limami A.M., Rentsch D., Seo M., Tsay Y.F., Zhang M.,
RA Coruzzi G., Lacombe B.;
RT "A unified nomenclature of NITRATE TRANSPORTER 1/PEPTIDE TRANSPORTER family
RT members in plants.";
RL Trends Plant Sci. 19:5-9(2014).
CC -!- FUNCTION: Low-affinity nitrate transporter. Involved in nitrate removal
CC from xylem sap. Not involved in oligopeptides transport.
CC {ECO:0000269|PubMed:20501909}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:20501909};
CC Multi-pass membrane protein {ECO:0000269|PubMed:20501909}.
CC -!- TISSUE SPECIFICITY: Expressed in xylem parenchyma cells within the
CC vasculature. Expressed in siliques and flowers. Higher expression in
CC shoots than in roots. {ECO:0000269|PubMed:17481610,
CC ECO:0000269|PubMed:20501909}.
CC -!- INDUCTION: Up-regulated by cadmium and nitrate.
CC {ECO:0000269|PubMed:20501909}.
CC -!- DISRUPTION PHENOTYPE: No visible phenotype. Higher nitrate
CC concentration in xylem sap and increased cadmium sensitivity. A greater
CC proportion of nitrate accumulates in shoots under cadmium stress.
CC {ECO:0000269|PubMed:20501909}.
CC -!- SIMILARITY: Belongs to the major facilitator superfamily. Proton-
CC dependent oligopeptide transporter (POT/PTR) (TC 2.A.17) family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAB36812.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=CAB81275.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AL035527; CAB36812.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AL161555; CAB81275.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002687; AEE84489.1; -; Genomic_DNA.
DR EMBL; AK118142; BAC42767.1; -; mRNA.
DR PIR; T05843; T05843.
DR RefSeq; NP_193899.2; NM_118288.4.
DR AlphaFoldDB; Q8GXN2; -.
DR SMR; Q8GXN2; -.
DR BioGRID; 13544; 25.
DR IntAct; Q8GXN2; 25.
DR STRING; 3702.AT4G21680.1; -.
DR PaxDb; Q8GXN2; -.
DR PRIDE; Q8GXN2; -.
DR ProteomicsDB; 226428; -.
DR EnsemblPlants; AT4G21680.1; AT4G21680.1; AT4G21680.
DR GeneID; 828255; -.
DR Gramene; AT4G21680.1; AT4G21680.1; AT4G21680.
DR KEGG; ath:AT4G21680; -.
DR Araport; AT4G21680; -.
DR TAIR; locus:2119058; AT4G21680.
DR eggNOG; KOG1237; Eukaryota.
DR HOGENOM; CLU_009313_4_1_1; -.
DR InParanoid; Q8GXN2; -.
DR OMA; HEEFTLD; -.
DR OrthoDB; 365203at2759; -.
DR PhylomeDB; Q8GXN2; -.
DR PRO; PR:Q8GXN2; -.
DR Proteomes; UP000006548; Chromosome 4.
DR ExpressionAtlas; Q8GXN2; baseline and differential.
DR Genevisible; Q8GXN2; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IDA:TAIR.
DR GO; GO:0015112; F:nitrate transmembrane transporter activity; IDA:TAIR.
DR GO; GO:0015293; F:symporter activity; IEA:UniProtKB-KW.
DR GO; GO:0042128; P:nitrate assimilation; IEA:UniProtKB-KW.
DR GO; GO:0046686; P:response to cadmium ion; IEP:TAIR.
DR GO; GO:0010167; P:response to nitrate; IEP:TAIR.
DR Gene3D; 1.20.1250.20; -; 1.
DR InterPro; IPR036259; MFS_trans_sf.
DR InterPro; IPR000109; POT_fam.
DR PANTHER; PTHR11654; PTHR11654; 1.
DR Pfam; PF00854; PTR2; 1.
DR SUPFAM; SSF103473; SSF103473; 1.
PE 2: Evidence at transcript level;
KW Cell membrane; Membrane; Nitrate assimilation; Phosphoprotein;
KW Reference proteome; Symport; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..589
FT /note="Protein NRT1/ PTR FAMILY 7.2"
FT /id="PRO_0000399981"
FT TRANSMEM 32..52
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 78..98
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 105..125
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 147..167
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 187..207
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 217..237
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 343..363
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 377..397
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 423..443
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 464..484
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 504..524
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 548..568
FT /note="Helical"
FT /evidence="ECO:0000255"
FT MOD_RES 102
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:Q05085"
FT CONFLICT 389
FT /note="S -> N (in Ref. 3; BAC42767)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 589 AA; 65507 MW; 0FEF4CF5162067F0 CRC64;
MDQKVRQFEV CTQDGSVDRH GNPAIRANTG KWLTAILILV NQGLATLAFF GVGVNLVLFL
TRVMGQDNAE AANNVSKWTG TVYIFSLLGA FLSDSYWGRY KTCAIFQASF VAGLMMLSLS
TGALLLEPSG CGVEDSPCKP HSTFKTVLFY LSVYLIALGY GGYQPNIATF GADQFDAEDS
VEGHSKIAFF SYFYLALNLG SLFSNTVLGY FEDQGEWPLG FWASAGSAFA GLVLFLIGTP
KYRHFTPRES PWSRFCQVLV AATRKAKIDV HHEELNLYDS ETQYTGDKKI LHTKGFRFLD
RAAIVTPDDE AEKVESGSKY DPWRLCSVTQ VEEVKCVLRL LPIWLCTILY SVVFTQMASL
FVVQGAAMKT NIKNFRIPAS SMSSFDILSV AFFIFAYRRF LDPLFARLNK TERNKGLTEL
QRMGIGLVIA IMAMISAGIV EIHRLKNKEP ESATSISSSS TLSIFWQVPQ YMLIGASEVF
MYVGQLEFFN SQAPTGLKSF ASALCMASIS LGNYVSSLLV SIVMKISTTD DVHGWIPENL
NKGHLERFYF LLAGLTAADF VVYLICAKWY KYIKSEASFS ESVTEEEEV
