PTR53_ARATH
ID PTR53_ARATH Reviewed; 616 AA.
AC Q9LV10;
DT 02-NOV-2010, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 109.
DE RecName: Full=Protein NRT1/ PTR FAMILY 2.11;
DE Short=AtNPF2.11;
DE AltName: Full=Nitrate transporter 1.10;
DE AltName: Full=Protein GLUCOSINOLATE TRANSPORTER-2;
GN Name=NPF2.11; Synonyms=GTR2, NRT1.10; OrderedLocusNames=At5g62680;
GN ORFNames=MRG21.10;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10718197; DOI=10.1093/dnares/7.1.31;
RA Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Kotani H.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. X. Sequence
RT features of the regions of 3,076,755 bp covered by sixty P1 and TAC
RT clones.";
RL DNA Res. 7:31-63(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=19423640; DOI=10.1093/dnares/dsp009;
RA Iida K., Fukami-Kobayashi K., Toyoda A., Sakaki Y., Kobayashi M., Seki M.,
RA Shinozaki K.;
RT "Analysis of multiple occurrences of alternative splicing events in
RT Arabidopsis thaliana using novel sequenced full-length cDNAs.";
RL DNA Res. 16:155-164(2009).
RN [4]
RP TISSUE SPECIFICITY, AND GENE FAMILY.
RX PubMed=17481610; DOI=10.1016/j.febslet.2007.04.047;
RA Tsay Y.F., Chiu C.C., Tsai C.B., Ho C.H., Hsu P.K.;
RT "Nitrate transporters and peptide transporters.";
RL FEBS Lett. 581:2290-2300(2007).
RN [5]
RP GENE FAMILY.
RX PubMed=20501909; DOI=10.1105/tpc.110.075242;
RA Li J.Y., Fu Y.L., Pike S.M., Bao J., Tian W., Zhang Y., Chen C.Z.,
RA Zhang Y., Li H.M., Huang J., Li L.G., Schroeder J.I., Gassmann W.,
RA Gong J.M.;
RT "The Arabidopsis nitrate transporter NRT1.8 functions in nitrate removal
RT from the xylem sap and mediates cadmium tolerance.";
RL Plant Cell 22:1633-1646(2010).
RN [6]
RP FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, DISRUPTION PHENOTYPE, SUBCELLULAR
RP LOCATION, AND TISSUE SPECIFICITY.
RX PubMed=22864417; DOI=10.1038/nature11285;
RA Nour-Eldin H.H., Andersen T.G., Burow M., Madsen S.R., Jorgensen M.E.,
RA Olsen C.E., Dreyer I., Hedrich R., Geiger D., Halkier B.A.;
RT "NRT/PTR transporters are essential for translocation of glucosinolate
RT defence compounds to seeds.";
RL Nature 488:531-534(2012).
RN [7]
RP FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=23995084; DOI=10.1105/tpc.113.110890;
RA Andersen T.G., Nour-Eldin H.H., Fuller V.L., Olsen C.E., Burow M.,
RA Halkier B.A.;
RT "Integration of biosynthesis and long-distance transport establish organ-
RT specific glucosinolate profiles in vegetative Arabidopsis.";
RL Plant Cell 25:3133-3145(2013).
RN [8]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=24055139; DOI=10.1016/j.tplants.2013.08.008;
RA Leran S., Varala K., Boyer J.C., Chiurazzi M., Crawford N.,
RA Daniel-Vedele F., David L., Dickstein R., Fernandez E., Forde B.,
RA Gassmann W., Geiger D., Gojon A., Gong J.M., Halkier B.A., Harris J.M.,
RA Hedrich R., Limami A.M., Rentsch D., Seo M., Tsay Y.F., Zhang M.,
RA Coruzzi G., Lacombe B.;
RT "A unified nomenclature of NITRATE TRANSPORTER 1/PEPTIDE TRANSPORTER family
RT members in plants.";
RL Trends Plant Sci. 19:5-9(2014).
CC -!- FUNCTION: High-affinity, proton-dependent glucosinolate-specific
CC transporter. Involved in apoplasmic phloem-loading of glucosinolates
CC and in bidirectional long-distance transport of aliphatic but not
CC indole glucosinolates. May be involved in removal of glucosinolates
CC from the xylem in roots. {ECO:0000269|PubMed:22864417,
CC ECO:0000269|PubMed:23995084}.
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC KM=18.7 uM for 4-methylthiobutyl glucosinolate
CC {ECO:0000269|PubMed:22864417};
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:22864417};
CC Multi-pass membrane protein {ECO:0000269|PubMed:22864417}.
CC -!- TISSUE SPECIFICITY: Expressed in roots. Detected in shoots, stems and
CC flowers. Expressed in veins and in the root vasculature with highest
CC expression in lateral branching points. {ECO:0000269|PubMed:17481610,
CC ECO:0000269|PubMed:22864417, ECO:0000269|PubMed:23995084}.
CC -!- DISRUPTION PHENOTYPE: 48% reduction in total glucosinolate levels in
CC seeds. Gtr1 and gtr2 double mutant has no detectable glucosinolate in
CC seeds. {ECO:0000269|PubMed:22864417}.
CC -!- SIMILARITY: Belongs to the major facilitator superfamily. Proton-
CC dependent oligopeptide transporter (POT/PTR) (TC 2.A.17) family.
CC {ECO:0000305}.
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DR EMBL; AB020751; BAA97215.1; -; Genomic_DNA.
DR EMBL; CP002688; AED97642.1; -; Genomic_DNA.
DR EMBL; AK316918; BAH19623.1; -; mRNA.
DR RefSeq; NP_201074.1; NM_125663.4.
DR AlphaFoldDB; Q9LV10; -.
DR SMR; Q9LV10; -.
DR STRING; 3702.AT5G62680.1; -.
DR TCDB; 2.A.17.3.20; the proton-dependent oligopeptide transporter (pot/ptr) family.
DR PaxDb; Q9LV10; -.
DR PRIDE; Q9LV10; -.
DR ProteomicsDB; 226446; -.
DR EnsemblPlants; AT5G62680.1; AT5G62680.1; AT5G62680.
DR GeneID; 836389; -.
DR Gramene; AT5G62680.1; AT5G62680.1; AT5G62680.
DR KEGG; ath:AT5G62680; -.
DR Araport; AT5G62680; -.
DR TAIR; locus:2172249; AT5G62680.
DR eggNOG; KOG1237; Eukaryota.
DR HOGENOM; CLU_009313_4_2_1; -.
DR InParanoid; Q9LV10; -.
DR OMA; VWATCIV; -.
DR OrthoDB; 365203at2759; -.
DR PhylomeDB; Q9LV10; -.
DR PRO; PR:Q9LV10; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9LV10; baseline and differential.
DR Genevisible; Q9LV10; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IDA:TAIR.
DR GO; GO:0009506; C:plasmodesma; HDA:TAIR.
DR GO; GO:0090448; F:glucosinolate:proton symporter activity; IDA:TAIR.
DR GO; GO:1901349; P:glucosinolate transport; IDA:TAIR.
DR GO; GO:0090449; P:phloem glucosinolate loading; IMP:TAIR.
DR Gene3D; 1.20.1250.20; -; 1.
DR InterPro; IPR036259; MFS_trans_sf.
DR InterPro; IPR000109; POT_fam.
DR PANTHER; PTHR11654; PTHR11654; 1.
DR Pfam; PF00854; PTR2; 1.
DR SUPFAM; SSF103473; SSF103473; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..616
FT /note="Protein NRT1/ PTR FAMILY 2.11"
FT /id="PRO_0000399987"
FT TRANSMEM 59..79
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 87..107
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 118..138
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 159..179
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 205..225
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 233..253
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 349..369
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 392..412
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 435..455
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 483..503
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 519..539
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 566..586
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 1..22
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 616 AA; 67891 MW; 8D545D2664C3115C CRC64;
MERKPLELES TDNHQNPSSA VYGGSVTAVD SVEEDVQNQK KVVYRGWKVM PFIIGNETFE
KLGIIGTLSN LLVYLTAVFN LKSITAATII NAFSGTINFG TFVAAFLCDT YFGRYKTLSV
AVIACFLGSF VILLTAAVPQ LHPAACGTAA DSICNGPSGG QIAFLLMGLG FLVVGAGGIR
PCNLAFGADQ FNPKSESGKR GIDSFFNWYF FTFTFAQILS LTLVVYVQSN VSWTIGLTIP
AVLMFLACLI FFAGDKLYVK IKASGSPLAG IAQVIAVAIK KRGLKPAKQP WLNLYNYYPP
KYANSKLKYT DQFRFLDKAA ILTPEDKLQP DGKPADPWKL CTMQQVEEVK CIVRVLPIWF
ASSIYYLTIT QQMTYPVFQA LQSDRRLGSG GFVIPAATYV VFLMTGMTVF IVVYDRVLVP
TMRRITGLDT GITLLQRIGT GIFFATASLV VAGFVEERRR TFALTKPTLG MAPRKGEISS
MSAMWLIPQL SLAGVAEAFA AIGQMEFYYK QFPENMRSFA GSIFYVGGGV SSYLGSFLIA
TVHRTTQNSS GGNWLAEDLN KGRLDLFYFM IAGILAVNFA YFLVMSRWYR YKGSDDEVTT
YETNENIIKQ QDKNVA
