PTR5_ARATH
ID PTR5_ARATH Reviewed; 570 AA.
AC Q9LFB8; Q0WR84; Q84TJ5;
DT 16-JUN-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 124.
DE RecName: Full=Protein NRT1/ PTR FAMILY 8.2;
DE Short=AtNPF8.2;
DE AltName: Full=Peptide transporter PTR5;
GN Name=NPF8.2; Synonyms=PTR5; OrderedLocusNames=At5g01180; ORFNames=F7J8.160;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130714; DOI=10.1038/35048507;
RA Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E.,
RA Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M.,
RA Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.,
RA Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M.,
RA Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L.,
RA O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D.,
RA Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M.,
RA Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L.,
RA Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B.,
RA Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P.,
RA Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M.,
RA Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D.,
RA Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A.,
RA Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I.,
RA Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T.,
RA Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A.,
RA McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U.,
RA Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W.,
RA Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M.,
RA Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S.,
RA Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G.,
RA Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W.,
RA Bevan M., Fransz P.F.;
RT "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.";
RL Nature 408:823-826(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 59-570.
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 60-570.
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP TISSUE SPECIFICITY, AND GENE FAMILY.
RX PubMed=17481610; DOI=10.1016/j.febslet.2007.04.047;
RA Tsay Y.F., Chiu C.C., Tsai C.B., Ho C.H., Hsu P.K.;
RT "Nitrate transporters and peptide transporters.";
RL FEBS Lett. 581:2290-2300(2007).
RN [6]
RP FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE,
RP AND DISRUPTION PHENOTYPE.
RX PubMed=18753286; DOI=10.1104/pp.108.123844;
RA Komarova N.Y., Thor K., Gubler A., Meier S., Dietrich D., Weichert A.,
RA Suter Grotemeyer M., Tegeder M., Rentsch D.;
RT "AtPTR1 and AtPTR5 transport dipeptides in planta.";
RL Plant Physiol. 148:856-869(2008).
RN [7]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=24055139; DOI=10.1016/j.tplants.2013.08.008;
RA Leran S., Varala K., Boyer J.C., Chiurazzi M., Crawford N.,
RA Daniel-Vedele F., David L., Dickstein R., Fernandez E., Forde B.,
RA Gassmann W., Geiger D., Gojon A., Gong J.M., Halkier B.A., Harris J.M.,
RA Hedrich R., Limami A.M., Rentsch D., Seo M., Tsay Y.F., Zhang M.,
RA Coruzzi G., Lacombe B.;
RT "A unified nomenclature of NITRATE TRANSPORTER 1/PEPTIDE TRANSPORTER family
RT members in plants.";
RL Trends Plant Sci. 19:5-9(2014).
CC -!- FUNCTION: Peptide transporter. Mediates the transport of di- and
CC tripeptides. High affinity transporter. Involved in the uptake of
CC peptides during pollen germination and tube growth.
CC {ECO:0000269|PubMed:18753286}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:18753286};
CC Multi-pass membrane protein {ECO:0000269|PubMed:18753286}.
CC -!- TISSUE SPECIFICITY: Expressed in developing and germinating pollen
CC grains and ovules. {ECO:0000269|PubMed:17481610,
CC ECO:0000269|PubMed:18753286}.
CC -!- DEVELOPMENTAL STAGE: Expressed during early seed development.
CC {ECO:0000269|PubMed:18753286}.
CC -!- DISRUPTION PHENOTYPE: No visible phenotype.
CC {ECO:0000269|PubMed:18753286}.
CC -!- SIMILARITY: Belongs to the major facilitator superfamily. Proton-
CC dependent oligopeptide transporter (POT/PTR) (TC 2.A.17) family.
CC {ECO:0000305}.
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DR EMBL; AL137189; CAB69846.1; -; Genomic_DNA.
DR EMBL; CP002688; AED90307.1; -; Genomic_DNA.
DR EMBL; CP002688; ANM69606.1; -; Genomic_DNA.
DR EMBL; CP002688; ANM69607.1; -; Genomic_DNA.
DR EMBL; BT005728; AAO64143.1; -; mRNA.
DR EMBL; AK228435; BAF00365.1; -; mRNA.
DR PIR; T45958; T45958.
DR RefSeq; NP_001331271.1; NM_001342576.1.
DR RefSeq; NP_001331272.1; NM_001342577.1.
DR RefSeq; NP_195738.1; NM_120196.4.
DR AlphaFoldDB; Q9LFB8; -.
DR SMR; Q9LFB8; -.
DR BioGRID; 17001; 25.
DR IntAct; Q9LFB8; 25.
DR STRING; 3702.AT5G01180.1; -.
DR TCDB; 2.A.17.3.8; the proton-dependent oligopeptide transporter (pot/ptr) family.
DR PaxDb; Q9LFB8; -.
DR PRIDE; Q9LFB8; -.
DR ProteomicsDB; 224840; -.
DR EnsemblPlants; AT5G01180.1; AT5G01180.1; AT5G01180.
DR EnsemblPlants; AT5G01180.2; AT5G01180.2; AT5G01180.
DR EnsemblPlants; AT5G01180.3; AT5G01180.3; AT5G01180.
DR GeneID; 831725; -.
DR Gramene; AT5G01180.1; AT5G01180.1; AT5G01180.
DR Gramene; AT5G01180.2; AT5G01180.2; AT5G01180.
DR Gramene; AT5G01180.3; AT5G01180.3; AT5G01180.
DR KEGG; ath:AT5G01180; -.
DR Araport; AT5G01180; -.
DR TAIR; locus:2150124; AT5G01180.
DR eggNOG; KOG1237; Eukaryota.
DR HOGENOM; CLU_009313_4_1_1; -.
DR InParanoid; Q9LFB8; -.
DR OMA; RPGWIAK; -.
DR OrthoDB; 365203at2759; -.
DR PhylomeDB; Q9LFB8; -.
DR BRENDA; 7.4.2.5; 399.
DR PRO; PR:Q9LFB8; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9LFB8; baseline and differential.
DR Genevisible; Q9LFB8; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IDA:TAIR.
DR GO; GO:0071916; F:dipeptide transmembrane transporter activity; IDA:TAIR.
DR GO; GO:0042937; F:tripeptide transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0042938; P:dipeptide transport; IDA:TAIR.
DR GO; GO:0009860; P:pollen tube growth; IMP:TAIR.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR Gene3D; 1.20.1250.20; -; 1.
DR InterPro; IPR036259; MFS_trans_sf.
DR InterPro; IPR000109; POT_fam.
DR InterPro; IPR018456; PTR2_symporter_CS.
DR PANTHER; PTHR11654; PTHR11654; 1.
DR Pfam; PF00854; PTR2; 1.
DR SUPFAM; SSF103473; SSF103473; 1.
DR PROSITE; PS01022; PTR2_1; 1.
DR PROSITE; PS01023; PTR2_2; 1.
PE 2: Evidence at transcript level;
KW Cell membrane; Membrane; Peptide transport; Phosphoprotein;
KW Protein transport; Reference proteome; Transmembrane; Transmembrane helix;
KW Transport.
FT CHAIN 1..570
FT /note="Protein NRT1/ PTR FAMILY 8.2"
FT /id="PRO_0000378323"
FT TRANSMEM 100..120
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 136..156
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 182..202
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 210..230
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 335..355
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 370..390
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 414..434
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 454..474
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 493..513
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 537..557
FT /note="Helical"
FT /evidence="ECO:0000255"
FT MOD_RES 99
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:Q05085"
FT CONFLICT 302
FT /note="T -> A (in Ref. 3; AAO64143 and 4; BAF00365)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 570 AA; 63308 MW; 4603B9B64BB20CF5 CRC64;
MEDDKDIYTK DGTLDIHKKP ANKNKTGTWK ACRFILGTEC CERLAYYGMS TNLINYLEKQ
MNMENVSASK SVSNWSGTCY ATPLIGAFIA DAYLGRYWTI ASFVVIYIAG MTLLTISASV
PGLTPTCSGE TCHATAGQTA ITFIALYLIA LGTGGIKPCV SSFGADQFDD TDEKEKESKS
SFFNWFYFVI NVGAMIASSV LVWIQMNVGW GWGLGVPTVA MAIAVVFFFA GSNFYRLQKP
GGSPLTRMLQ VIVASCRKSK VKIPEDESLL YENQDAESSI IGSRKLEHTK ILTFFDKAAV
ETESDNKGAA KSSSWKLCTV TQVEELKALI RLLPIWATGI VFASVYSQMG TVFVLQGNTL
DQHMGPNFKI PSASLSLFDT LSVLFWAPVY DKLIVPFARK YTGHERGFTQ LQRIGIGLVI
SIFSMVSAGI LEVARLNYVQ THNLYNEETI PMTIFWQVPQ YFLVGCAEVF TFIGQLEFFY
DQAPDAMRSL CSALSLTAIA FGNYLSTFLV TLVTKVTRSG GRPGWIAKNL NNGHLDYFFW
LLAGLSFLNF LVYLWIAKWY TYKKTTGHAL
