PTR6_ARATH
ID PTR6_ARATH Reviewed; 607 AA.
AC Q9M817; Q8LFV0; Q93Z23;
DT 18-MAR-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 117.
DE RecName: Full=Protein NRT1/ PTR FAMILY 1.2;
DE Short=AtNPF1.2;
DE AltName: Full=Nitrate transporter 1.11;
GN Name=NPF1.2; Synonyms=NRT1.11; OrderedLocusNames=At1g52190;
GN ORFNames=F9I5.4;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-292.
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 284-607.
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP TISSUE SPECIFICITY, AND GENE FAMILY.
RX PubMed=17481610; DOI=10.1016/j.febslet.2007.04.047;
RA Tsay Y.F., Chiu C.C., Tsai C.B., Ho C.H., Hsu P.K.;
RT "Nitrate transporters and peptide transporters.";
RL FEBS Lett. 581:2290-2300(2007).
RN [6]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-601, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Seedling;
RX PubMed=17586839; DOI=10.1074/mcp.m700164-mcp200;
RA Niittylae T., Fuglsang A.T., Palmgren M.G., Frommer W.B., Schulze W.X.;
RT "Temporal analysis of sucrose-induced phosphorylation changes in plasma
RT membrane proteins of Arabidopsis.";
RL Mol. Cell. Proteomics 6:1711-1726(2007).
RN [7]
RP FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY, SUBCELLULAR
RP LOCATION, AND DISRUPTION PHENOTYPE.
RX PubMed=24006285; DOI=10.1104/pp.113.226563;
RA Hsu P.K., Tsay Y.F.;
RT "Two phloem nitrate transporters, NRT1.11 and NRT1.12, are important for
RT redistributing xylem-borne nitrate to enhance plant growth.";
RL Plant Physiol. 163:844-856(2013).
RN [8]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=24055139; DOI=10.1016/j.tplants.2013.08.008;
RA Leran S., Varala K., Boyer J.C., Chiurazzi M., Crawford N.,
RA Daniel-Vedele F., David L., Dickstein R., Fernandez E., Forde B.,
RA Gassmann W., Geiger D., Gojon A., Gong J.M., Halkier B.A., Harris J.M.,
RA Hedrich R., Limami A.M., Rentsch D., Seo M., Tsay Y.F., Zhang M.,
RA Coruzzi G., Lacombe B.;
RT "A unified nomenclature of NITRATE TRANSPORTER 1/PEPTIDE TRANSPORTER family
RT members in plants.";
RL Trends Plant Sci. 19:5-9(2014).
CC -!- FUNCTION: Low-affinity nitrate transporter involved in xylem-to-phloem
CC transfer for redistributing nitrate into developing leaves. Not
CC involved in dipeptides transport. {ECO:0000269|PubMed:24006285}.
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC KM=7.2 mM for nitrate {ECO:0000269|PubMed:24006285};
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:24006285};
CC Multi-pass membrane protein {ECO:0000269|PubMed:24006285}.
CC -!- TISSUE SPECIFICITY: Expressed in shoots, stems, leaves, flowers and
CC siliques. Mainly detected in larger expanded leaves, in the companion
CC cells of major veins. {ECO:0000269|PubMed:17481610,
CC ECO:0000269|PubMed:24006285}.
CC -!- DISRUPTION PHENOTYPE: Nrt1.11 and nrt1.12 double mutant is defective in
CC high-nitrate-enhanced growth. {ECO:0000269|PubMed:24006285}.
CC -!- SIMILARITY: Belongs to the major facilitator superfamily. Proton-
CC dependent oligopeptide transporter (POT/PTR) (TC 2.A.17) family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAL25616.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AC022354; AAF29404.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE32766.1; -; Genomic_DNA.
DR EMBL; AY084631; AAM61194.1; -; mRNA.
DR EMBL; AY058204; AAL25616.1; ALT_INIT; mRNA.
DR EMBL; AY098981; AAM19991.1; -; mRNA.
DR PIR; H96561; H96561.
DR RefSeq; NP_175630.1; NM_104099.4.
DR AlphaFoldDB; Q9M817; -.
DR SMR; Q9M817; -.
DR BioGRID; 26874; 4.
DR IntAct; Q9M817; 4.
DR STRING; 3702.AT1G52190.1; -.
DR TCDB; 2.A.17.3.13; the proton-dependent oligopeptide transporter (pot/ptr) family.
DR iPTMnet; Q9M817; -.
DR PaxDb; Q9M817; -.
DR PRIDE; Q9M817; -.
DR ProteomicsDB; 226430; -.
DR EnsemblPlants; AT1G52190.1; AT1G52190.1; AT1G52190.
DR GeneID; 841649; -.
DR Gramene; AT1G52190.1; AT1G52190.1; AT1G52190.
DR KEGG; ath:AT1G52190; -.
DR Araport; AT1G52190; -.
DR TAIR; locus:2037452; AT1G52190.
DR eggNOG; KOG1237; Eukaryota.
DR HOGENOM; CLU_009313_4_2_1; -.
DR InParanoid; Q9M817; -.
DR OMA; IMMSINT; -.
DR OrthoDB; 365203at2759; -.
DR PhylomeDB; Q9M817; -.
DR SABIO-RK; Q9M817; -.
DR PRO; PR:Q9M817; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9M817; baseline and differential.
DR Genevisible; Q9M817; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005634; C:nucleus; HDA:TAIR.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0080054; F:low-affinity nitrate transmembrane transporter activity; IDA:TAIR.
DR GO; GO:0015706; P:nitrate transmembrane transport; IDA:TAIR.
DR Gene3D; 1.20.1250.20; -; 1.
DR InterPro; IPR036259; MFS_trans_sf.
DR InterPro; IPR000109; POT_fam.
DR PANTHER; PTHR11654; PTHR11654; 1.
DR Pfam; PF00854; PTR2; 1.
DR SUPFAM; SSF103473; SSF103473; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Membrane; Phosphoprotein; Reference proteome; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..607
FT /note="Protein NRT1/ PTR FAMILY 1.2"
FT /id="PRO_0000324123"
FT TRANSMEM 65..85
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 96..116
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 138..158
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 185..205
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 215..235
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 374..394
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 418..438
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 460..480
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 496..516
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 544..564
FT /note="Helical"
FT /evidence="ECO:0000255"
FT MOD_RES 601
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:17586839"
FT CONFLICT 22
FT /note="K -> M (in Ref. 3; AAM61194)"
FT /evidence="ECO:0000305"
FT CONFLICT 244
FT /note="T -> S (in Ref. 3; AAM61194)"
FT /evidence="ECO:0000305"
FT CONFLICT 273
FT /note="F -> I (in Ref. 3; AAM61194)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 607 AA; 66904 MW; B8355650ADF0FBE4 CRC64;
MENPPNETEA KQIQTNEGKK TKGGIITMPF IIANEAFEKV ASYGLLPNMI MYLIRDYRFG
VAKGTNVLFM WSAASNFTPL LGAFLSDSYL GRFLTISIAS LSSFLGMVLL WLTAMLPQVK
PSPCDPTAAG SHCGSSTASQ LALLYSAFAL ISIGSGGIRP CSLAFGADQL DNKENPKNER
VLESFFGWYY ASSAVAVLIA FTGIVYIQEH LGWKIGFGVP AVLMLIAALL FILASPLYVT
RGVTKSLFTG LAQAIVAAYK KRKLSLPDHH DSFDCYYHMK DSEIKAPSQK LRFLNKACLI
SNREEEIGSD GFALNPWRLC TTDKVEELKA LIKVIPIWST GIMMSINTSQ SSFQLLQATS
MDRRLSRHGS SFQVPAGSFG MFTIIALALW VILYDRAVIP LASKIRGRPF RLSVKLRMGL
GLFMSFLAMA ISAMVESFRR KKAISQGYAN NSNAVVDISA MWLVPQYVLH GLAEALTAIG
QTEFFYTEFP KSMSSIAASL FGLGMAVASL LASVVLNAVN ELTSRNGKES WVSDNINKGH
YNYYYWVLAI MSFINVIYYV ICSWSYGPLV DQVRNGRVNG VREEEELIDI VGKGFEKEDL
SPVVKTN
