PTR8_ARATH
ID PTR8_ARATH Reviewed; 587 AA.
AC Q9M9V7;
DT 02-NOV-2010, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 110.
DE RecName: Full=Protein NRT1/ PTR FAMILY 2.9;
DE Short=AtNPF2.9;
DE AltName: Full=Nitrate transporter 1.9;
GN Name=NPF2.9; Synonyms=NRT1.9; OrderedLocusNames=At1g18880;
GN ORFNames=F6A14.2;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP TISSUE SPECIFICITY, AND GENE FAMILY.
RX PubMed=17481610; DOI=10.1016/j.febslet.2007.04.047;
RA Tsay Y.F., Chiu C.C., Tsai C.B., Ho C.H., Hsu P.K.;
RT "Nitrate transporters and peptide transporters.";
RL FEBS Lett. 581:2290-2300(2007).
RN [5]
RP GENE FAMILY.
RX PubMed=20501909; DOI=10.1105/tpc.110.075242;
RA Li J.Y., Fu Y.L., Pike S.M., Bao J., Tian W., Zhang Y., Chen C.Z.,
RA Zhang Y., Li H.M., Huang J., Li L.G., Schroeder J.I., Gassmann W.,
RA Gong J.M.;
RT "The Arabidopsis nitrate transporter NRT1.8 functions in nitrate removal
RT from the xylem sap and mediates cadmium tolerance.";
RL Plant Cell 22:1633-1646(2010).
RN [6]
RP FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY, INDUCTION BY
RP NITRATE, SUBCELLULAR LOCATION, AND DISRUPTION PHENOTYPE.
RX PubMed=21571952; DOI=10.1105/tpc.111.083618;
RA Wang Y.Y., Tsay Y.F.;
RT "Arabidopsis nitrate transporter NRT1.9 is important in phloem nitrate
RT transport.";
RL Plant Cell 23:1945-1957(2011).
RN [7]
RP FUNCTION.
RX PubMed=22864417; DOI=10.1038/nature11285;
RA Nour-Eldin H.H., Andersen T.G., Burow M., Madsen S.R., Jorgensen M.E.,
RA Olsen C.E., Dreyer I., Hedrich R., Geiger D., Halkier B.A.;
RT "NRT/PTR transporters are essential for translocation of glucosinolate
RT defence compounds to seeds.";
RL Nature 488:531-534(2012).
RN [8]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=24055139; DOI=10.1016/j.tplants.2013.08.008;
RA Leran S., Varala K., Boyer J.C., Chiurazzi M., Crawford N.,
RA Daniel-Vedele F., David L., Dickstein R., Fernandez E., Forde B.,
RA Gassmann W., Geiger D., Gojon A., Gong J.M., Halkier B.A., Harris J.M.,
RA Hedrich R., Limami A.M., Rentsch D., Seo M., Tsay Y.F., Zhang M.,
RA Coruzzi G., Lacombe B.;
RT "A unified nomenclature of NITRATE TRANSPORTER 1/PEPTIDE TRANSPORTER family
RT members in plants.";
RL Trends Plant Sci. 19:5-9(2014).
CC -!- FUNCTION: Low-affinity nitrate transporter facilitating nitrate loading
CC into root phloem. Not involved in dipeptides transport, but has a weak
CC glucosinolate transport activity. {ECO:0000269|PubMed:21571952,
CC ECO:0000269|PubMed:22864417}.
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC KM=7 mM for nitrate (at pH 5.5) {ECO:0000269|PubMed:21571952};
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:21571952};
CC Multi-pass membrane protein {ECO:0000269|PubMed:21571952}.
CC -!- TISSUE SPECIFICITY: Expressed in roots, stems and major veins of the
CC leaves. Detected in the companion cells of the root phloem.
CC {ECO:0000269|PubMed:17481610, ECO:0000269|PubMed:21571952}.
CC -!- INDUCTION: Up-regulated by nitrate after long time exposure.
CC {ECO:0000269|PubMed:21571952}.
CC -!- DISRUPTION PHENOTYPE: Decreased nitrate content in root phloem
CC exudates. Enhanced root-to shoot nitrate transport and plant growth
CC under high nitrate conditions. {ECO:0000269|PubMed:21571952}.
CC -!- SIMILARITY: Belongs to the major facilitator superfamily. Proton-
CC dependent oligopeptide transporter (POT/PTR) (TC 2.A.17) family.
CC {ECO:0000305}.
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DR EMBL; AC011809; AAF27093.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE29776.1; -; Genomic_DNA.
DR EMBL; AY070054; AAL49811.1; -; mRNA.
DR EMBL; AY117308; AAM51383.1; -; mRNA.
DR PIR; G86322; G86322.
DR RefSeq; NP_173322.1; NM_101745.5.
DR AlphaFoldDB; Q9M9V7; -.
DR SMR; Q9M9V7; -.
DR BioGRID; 23708; 26.
DR IntAct; Q9M9V7; 26.
DR STRING; 3702.AT1G18880.1; -.
DR iPTMnet; Q9M9V7; -.
DR PaxDb; Q9M9V7; -.
DR PRIDE; Q9M9V7; -.
DR ProteomicsDB; 226448; -.
DR EnsemblPlants; AT1G18880.1; AT1G18880.1; AT1G18880.
DR GeneID; 838469; -.
DR Gramene; AT1G18880.1; AT1G18880.1; AT1G18880.
DR KEGG; ath:AT1G18880; -.
DR Araport; AT1G18880; -.
DR TAIR; locus:2035005; AT1G18880.
DR eggNOG; KOG1237; Eukaryota.
DR HOGENOM; CLU_009313_4_2_1; -.
DR InParanoid; Q9M9V7; -.
DR OMA; NSKLGHT; -.
DR OrthoDB; 365203at2759; -.
DR PhylomeDB; Q9M9V7; -.
DR PRO; PR:Q9M9V7; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9M9V7; baseline and differential.
DR Genevisible; Q9M9V7; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IDA:TAIR.
DR GO; GO:0015112; F:nitrate transmembrane transporter activity; IDA:TAIR.
DR GO; GO:0090408; P:phloem nitrate loading; IMP:TAIR.
DR GO; GO:0010233; P:phloem transport; IMP:TAIR.
DR Gene3D; 1.20.1250.20; -; 1.
DR InterPro; IPR036259; MFS_trans_sf.
DR InterPro; IPR000109; POT_fam.
DR PANTHER; PTHR11654; PTHR11654; 1.
DR Pfam; PF00854; PTR2; 1.
DR SUPFAM; SSF103473; SSF103473; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..587
FT /note="Protein NRT1/ PTR FAMILY 2.9"
FT /id="PRO_0000399942"
FT TRANSMEM 35..55
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 65..85
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 94..114
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 135..155
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 181..201
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 209..229
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 325..345
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 368..388
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 412..432
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 457..477
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 493..513
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 540..560
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 587 AA; 65174 MW; 414A9C44C963BEDC CRC64;
MEVEKTEKNI AEDDESKIIY RGWKVMPFII GNETFEKLGI VGSSSNLVIY LTTVFNMKSI
TAAKVVNIYG GTSNFGTIVA AFLCDSYFGR YKTLSFAMIA CFLGSVAMDL TAVIHPLHPA
QCAKEIGSVC NGPSIGQIMF LAGAMVLLVI GAGGIRPCNL PFGADQFDPK TKEGKRGIES
FFNWYFFTFT FAQMVSLTLI VYVQSNVSWS IGLAIPAILM LLGCIIFFAG SKLYVKVKAS
GSPIHSITRV IVVAIKKRRL KPVGPNELYN YIASDFKNSK LGHTEQFRFL DKSAIQTQDD
KLNKDGSPVD AWKLCSMQQV EEVKCVIRVL PVWLSAALFY LAYIQQTTYT IFQSLQSDRR
LGPGSFQIPA GSYTVFLMLG MTIFIPIYDR VLVPFLRKYT GRDGGITQLQ RVGAGLFLCI
TSMMVSAIVE QYRRKVALTK PTLGLAPRKG AISSMSGMWL IPQLVLMGIA DALAGVGQME
FYYKQFPENM RSFAGSLYYC GIGLASYLST FLLSAVHDTT EGFSGGSWLP EDLNKGRLEY
FYFLVAGMMT LNLAYFLLVS HWYRYKDVVA KDKDMDKTSA EFDKVSV
