PTRA_KLEPN
ID PTRA_KLEPN Reviewed; 135 AA.
AC P37080;
DT 01-JUN-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-1994, sequence version 1.
DT 03-AUG-2022, entry version 114.
DE RecName: Full=PTS system sorbose-specific EIIA component {ECO:0000303|PubMed:2287279};
DE AltName: Full=EIIA-Sor {ECO:0000303|PubMed:2287279};
DE AltName: Full=EIII-F-Sor;
DE AltName: Full=Sorbose-specific phosphotransferase enzyme IIA component {ECO:0000303|PubMed:2287279};
GN Name=sorF {ECO:0000303|PubMed:2287279};
OS Klebsiella pneumoniae.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
OX NCBI_TaxID=573;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION.
RC STRAIN=1033-5P14 / KAY2026;
RX PubMed=7947968; DOI=10.1016/0167-4838(94)90124-4;
RA Wehmeier U.F., Lengeler J.W.;
RT "Sequence of the sor-operon for L-sorbose utilization from Klebsiella
RT pneumoniae KAY2026.";
RL Biochim. Biophys. Acta 1208:348-351(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=7700234; DOI=10.1007/bf00298968;
RA Wehmeier U.F., Wohrl B.M., Lengeler J.W.;
RT "Molecular analysis of the phosphoenolpyruvate-dependent L-sorbose:
RT phosphotransferase system from Klebsiella pneumoniae and of its multidomain
RT structure.";
RL Mol. Gen. Genet. 246:610-618(1995).
RN [3]
RP FUNCTION, AND CATALYTIC ACTIVITY.
RC STRAIN=1033-5P14 / KAY2026;
RX PubMed=6361004; DOI=10.1128/jb.157.1.39-45.1984;
RA Sprenger G.A., Lengeler J.W.;
RT "L-Sorbose metabolism in Klebsiella pneumoniae and Sor+ derivatives of
RT Escherichia coli K-12 and chemotaxis toward sorbose.";
RL J. Bacteriol. 157:39-45(1984).
RN [4]
RP FUNCTION, AND INDUCTION.
RX PubMed=2287279; DOI=10.1111/j.1365-2958.1990.tb02067.x;
RA Woehrl B.M., Lengeler J.W.;
RT "Cloning and physical mapping of the sor genes for L-sorbose transport and
RT metabolism from Klebsiella pneumoniae.";
RL Mol. Microbiol. 4:1557-1565(1990).
CC -!- FUNCTION: The phosphoenolpyruvate-dependent sugar phosphotransferase
CC system (PTS), a major carbohydrate active transport system, catalyzes
CC the phosphorylation of incoming sugar substrates concomitant with their
CC translocation across the cell membrane. The enzyme II SorABFM PTS
CC system is involved in L-sorbose transport. {ECO:0000269|PubMed:6361004,
CC ECO:0000305|PubMed:2287279, ECO:0000305|PubMed:7700234}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC -!- INDUCTION: By L-sorbose. {ECO:0000269|PubMed:2287279}.
CC -!- DOMAIN: The EIIA type-4 domain is phosphorylated by phospho-HPr on a
CC histidyl residue. Then, it transfers the phosphoryl group to the EIIB
CC type-4 domain. {ECO:0000255|PROSITE-ProRule:PRU00419}.
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DR EMBL; X66059; CAA46857.1; -; Genomic_DNA.
DR PIR; S50187; S50187.
DR RefSeq; WP_002884611.1; NZ_WYAL01000049.1.
DR AlphaFoldDB; P37080; -.
DR SMR; P37080; -.
DR TCDB; 4.A.6.1.3; the pts mannose-fructose-sorbose (man) family.
DR GeneID; 56941192; -.
DR OMA; IVHNAQN; -.
DR OrthoDB; 2008912at2; -.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:InterPro.
DR GO; GO:0016301; F:kinase activity; IEA:UniProtKB-KW.
DR GO; GO:0016773; F:phosphotransferase activity, alcohol group as acceptor; IEA:InterPro.
DR GO; GO:0009401; P:phosphoenolpyruvate-dependent sugar phosphotransferase system; IEA:UniProtKB-KW.
DR GO; GO:0016310; P:phosphorylation; IEA:UniProtKB-KW.
DR CDD; cd00006; PTS_IIA_man; 1.
DR Gene3D; 3.40.50.510; -; 1.
DR InterPro; IPR013789; PTS_EIIA_man.
DR InterPro; IPR004701; PTS_EIIA_man-typ.
DR InterPro; IPR036662; PTS_EIIA_man-typ_sf.
DR InterPro; IPR033887; PTS_IIA_man.
DR Pfam; PF03610; EIIA-man; 1.
DR SUPFAM; SSF53062; SSF53062; 1.
DR TIGRFAMs; TIGR00824; EIIA-man; 1.
DR PROSITE; PS51096; PTS_EIIA_TYPE_4; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Kinase; Phosphoprotein; Phosphotransferase system;
KW Sugar transport; Transferase; Transport.
FT CHAIN 1..135
FT /note="PTS system sorbose-specific EIIA component"
FT /id="PRO_0000186665"
FT DOMAIN 1..131
FT /note="PTS EIIA type-4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00419"
FT ACT_SITE 9
FT /note="Tele-phosphohistidine intermediate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00419"
FT MOD_RES 9
FT /note="Phosphohistidine; by HPr"
FT /evidence="ECO:0000305"
SQ SEQUENCE 135 AA; 14508 MW; 8DEF06570D46B706 CRC64;
MVHAIFCAHG QLAGAMLDSV CMVYGEVNVS AVAFVPGENA ADIAINLEKL VSAHTDEEWV
IAVDLQCGSP WNAAAGLAMR HPQIRVISGL SLPLALELVD NQHTLSADDL CQHLQAIASQ
CCVVWQQPET VEEEF
