ID   PTSB_LACLL              Reviewed;          40 AA.
AC   Q04938;
DT   01-JUN-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-1994, sequence version 1.
DT   03-AUG-2022, entry version 89.
DE   RecName: Full=Sucrose-specific phosphotransferase enzyme IIB component;
DE            EC=2.7.1.-;
DE   AltName: Full=EII-Scr;
DE   AltName: Full=PTS system sucrose-specific EIIB component;
DE   Flags: Fragment;
GN   Name=sacB;
OS   Lactococcus lactis subsp. lactis (Streptococcus lactis).
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae;
OC   Lactococcus.
OX   NCBI_TaxID=1360;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=NIZO R5;
RX   PubMed=1330831; DOI=10.1016/0378-1119(92)90161-h;
RA   Rauch P.J., de Vos W.M.;
RT   "Transcriptional regulation of the Tn5276-located Lactococcus lactis
RT   sucrose operon and characterization of the sacA gene encoding sucrose-6-
RT   phosphate hydrolase.";
RL   Gene 121:55-61(1992).
CC   -!- FUNCTION: The phosphoenolpyruvate-dependent sugar phosphotransferase
CC       system (sugar PTS), a major carbohydrate active -transport system,
CC       catalyzes the phosphorylation of incoming sugar substrates
CC       concomitantly with their translocation across the cell membrane. This
CC       system is involved in sucrose transport.
CC   -!- DOMAIN: The EIIB domain is phosphorylated by phospho-EIIA on a
CC       cysteinyl or histidyl residue, depending on the transported sugar.
CC       Then, it transfers the phosphoryl group to the sugar substrate
CC       concomitantly with the sugar uptake processed by the EIIC domain.
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DR   EMBL; M96669; AAA25217.1; -; Genomic_DNA.
DR   PIR; JH0756; JH0756.
DR   AlphaFoldDB; Q04938; -.
DR   SMR; Q04938; -.
DR   GO; GO:0016301; F:kinase activity; IEA:UniProtKB-KW.
DR   GO; GO:0008982; F:protein-N(PI)-phosphohistidine-sugar phosphotransferase activity; IEA:InterPro.
DR   GO; GO:0009401; P:phosphoenolpyruvate-dependent sugar phosphotransferase system; IEA:UniProtKB-KW.
DR   GO; GO:0016310; P:phosphorylation; IEA:UniProtKB-KW.
DR   Gene3D; 3.30.1360.60; -; 1.
DR   InterPro; IPR036878; Glu_permease_IIB.
DR   InterPro; IPR018113; PTrfase_EIIB_Cys.
DR   InterPro; IPR001996; PTS_IIB_1.
DR   Pfam; PF00367; PTS_EIIB; 1.
DR   SUPFAM; SSF55604; SSF55604; 1.
DR   PROSITE; PS51098; PTS_EIIB_TYPE_1; 1.
DR   PROSITE; PS01035; PTS_EIIB_TYPE_1_CYS; 1.
PE   4: Predicted;
KW   Kinase; Phosphotransferase system; Sugar transport; Transferase; Transport.
FT   CHAIN           1..>40
FT                   /note="Sucrose-specific phosphotransferase enzyme IIB
FT                   component"
FT                   /id="PRO_0000186674"
FT   DOMAIN          1..>40
FT                   /note="PTS EIIB type-1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00421"
FT   ACT_SITE        25
FT                   /note="Phosphocysteine intermediate"
FT                   /evidence="ECO:0000250"
FT   NON_TER         40
SQ   SEQUENCE   40 AA;  4483 MW;  7BEF0BB5A5AD1A5F CRC64;
     MNHKQVAERI LNAVGRDNIQ GARHCATRLR LVLKDTGVID