ID   PTSN_BRADU              Reviewed;         153 AA.
AC   P30335;
DT   01-APR-1993, integrated into UniProtKB/Swiss-Prot.
DT   28-FEB-2003, sequence version 2.
DT   03-AUG-2022, entry version 121.
DE   RecName: Full=Nitrogen regulatory protein;
DE   AltName: Full=Enzyme IIA-NTR;
DE   AltName: Full=Nitrogen-metabolic PTS system EIIA component;
GN   Name=ptsN; OrderedLocusNames=blr0725;
OS   Bradyrhizobium diazoefficiens (strain JCM 10833 / BCRC 13528 / IAM 13628 /
OS   NBRC 14792 / USDA 110).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Bradyrhizobiaceae; Bradyrhizobium.
OX   NCBI_TaxID=224911;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=JCM 10833 / BCRC 13528 / IAM 13628 / NBRC 14792 / USDA 110;
RX   PubMed=12597275; DOI=10.1093/dnares/9.6.189;
RA   Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S.,
RA   Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M.,
RA   Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S.;
RT   "Complete genomic sequence of nitrogen-fixing symbiotic bacterium
RT   Bradyrhizobium japonicum USDA110.";
RL   DNA Res. 9:189-197(2002).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-90.
RC   STRAIN=USDA 110spc4;
RX   PubMed=1991712; DOI=10.1128/jb.173.3.1125-1138.1991;
RA   Kullik I., Fritsche S., Knobel H., Sanjuan J., Hennecke H., Fischer H.-M.;
RT   "Bradyrhizobium japonicum has two differentially regulated, functional
RT   homologs of the sigma 54 gene (rpoN).";
RL   J. Bacteriol. 173:1125-1138(1991).
CC   -!- FUNCTION: Seems to have a role in regulating nitrogen assimilation.
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm.
CC   -!- DOMAIN: The EIIA domain is phosphorylated by phospho-NPr on a histidyl
CC       residue.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; BA000040; BAC45990.1; -; Genomic_DNA.
DR   EMBL; M59243; AAA26243.1; -; Genomic_DNA.
DR   PIR; D38179; D38179.
DR   RefSeq; NP_767365.1; NC_004463.1.
DR   RefSeq; WP_011083550.1; NZ_CP011360.1.
DR   AlphaFoldDB; P30335; -.
DR   SMR; P30335; -.
DR   STRING; 224911.27348974; -.
DR   EnsemblBacteria; BAC45990; BAC45990; BAC45990.
DR   GeneID; 64020589; -.
DR   KEGG; bja:blr0725; -.
DR   PATRIC; fig|224911.44.peg.97; -.
DR   eggNOG; COG1762; Bacteria.
DR   HOGENOM; CLU_072531_5_2_5; -.
DR   InParanoid; P30335; -.
DR   OMA; FDAPDEQ; -.
DR   PhylomeDB; P30335; -.
DR   Proteomes; UP000002526; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0016301; F:kinase activity; IEA:UniProtKB-KW.
DR   GO; GO:0030295; F:protein kinase activator activity; IBA:GO_Central.
DR   GO; GO:0008982; F:protein-N(PI)-phosphohistidine-sugar phosphotransferase activity; IEA:InterPro.
DR   GO; GO:0009401; P:phosphoenolpyruvate-dependent sugar phosphotransferase system; IEA:InterPro.
DR   GO; GO:0016310; P:phosphorylation; IEA:UniProtKB-KW.
DR   GO; GO:0032412; P:regulation of ion transmembrane transporter activity; IBA:GO_Central.
DR   CDD; cd00211; PTS_IIA_fru; 1.
DR   Gene3D; 3.40.930.10; -; 1.
DR   InterPro; IPR016152; PTrfase/Anion_transptr.
DR   InterPro; IPR002178; PTS_EIIA_type-2_dom.
DR   InterPro; IPR006320; PTS_Nitro_regul.
DR   Pfam; PF00359; PTS_EIIA_2; 1.
DR   SUPFAM; SSF55804; SSF55804; 1.
DR   TIGRFAMs; TIGR01419; nitro_reg_IIA; 1.
DR   PROSITE; PS51094; PTS_EIIA_TYPE_2; 1.
DR   PROSITE; PS00372; PTS_EIIA_TYPE_2_HIS; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Kinase; Reference proteome; Transferase.
FT   CHAIN           1..153
FT                   /note="Nitrogen regulatory protein"
FT                   /id="PRO_0000186693"
FT   DOMAIN          5..148
FT                   /note="PTS EIIA type-2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00417"
FT   ACT_SITE        66
FT                   /note="Tele-phosphohistidine intermediate"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00417"
SQ   SEQUENCE   153 AA;  16455 MW;  9B52C7F34E2D491A CRC64;
     MPITDLVAPE AILPALKVNS KKQALQELAA KAAELTGQNE RAVFEVLLQR EKLGTTAVGY
     GVAIPHGKLP KLEKIFGLFA RLDRPIDFES MDGQPVDLVF LLLAPEGAGA DHLKALARIA
     RLLRDQDIAK KLRASRDAQA IYSVLALPPA TAA