ID   PTSN_PSEAE              Reviewed;         154 AA.
AC   Q9HVV4;
DT   15-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   03-AUG-2022, entry version 104.
DE   RecName: Full=Nitrogen regulatory protein;
DE   AltName: Full=Enzyme IIA-NTR;
DE   Includes:
DE     RecName: Full=Phosphotransferase enzyme IIA component;
DE     AltName: Full=PTS system EIIA component;
GN   Name=ptsN; OrderedLocusNames=PA4464;
OS   Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS   14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=208964;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC   PRS 101 / PAO1;
RX   PubMed=10984043; DOI=10.1038/35023079;
RA   Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA   Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA   Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA   Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA   Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA   Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT   "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT   pathogen.";
RL   Nature 406:959-964(2000).
CC   -!- FUNCTION: Seems to have a role in regulating nitrogen assimilation.
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- DOMAIN: The PTS EIIA type-2 domain may serve a regulatory function,
CC       through its phosphorylation activity. {ECO:0000250}.
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DR   EMBL; AE004091; AAG07852.1; -; Genomic_DNA.
DR   PIR; H83087; H83087.
DR   RefSeq; NP_253154.1; NC_002516.2.
DR   RefSeq; WP_003098853.1; NZ_QZGE01000004.1.
DR   AlphaFoldDB; Q9HVV4; -.
DR   SMR; Q9HVV4; -.
DR   STRING; 287.DR97_1643; -.
DR   PaxDb; Q9HVV4; -.
DR   PRIDE; Q9HVV4; -.
DR   EnsemblBacteria; AAG07852; AAG07852; PA4464.
DR   GeneID; 880997; -.
DR   KEGG; pae:PA4464; -.
DR   PATRIC; fig|208964.12.peg.4674; -.
DR   PseudoCAP; PA4464; -.
DR   HOGENOM; CLU_072531_5_2_6; -.
DR   InParanoid; Q9HVV4; -.
DR   OMA; FDAPDEQ; -.
DR   PhylomeDB; Q9HVV4; -.
DR   BioCyc; PAER208964:G1FZ6-4553-MON; -.
DR   Proteomes; UP000002438; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0016301; F:kinase activity; IEA:UniProtKB-KW.
DR   GO; GO:0030295; F:protein kinase activator activity; IBA:GO_Central.
DR   GO; GO:0008982; F:protein-N(PI)-phosphohistidine-sugar phosphotransferase activity; IEA:InterPro.
DR   GO; GO:0009401; P:phosphoenolpyruvate-dependent sugar phosphotransferase system; IEA:InterPro.
DR   GO; GO:0016310; P:phosphorylation; IEA:UniProtKB-KW.
DR   GO; GO:0032412; P:regulation of ion transmembrane transporter activity; IBA:GO_Central.
DR   CDD; cd00211; PTS_IIA_fru; 1.
DR   Gene3D; 3.40.930.10; -; 1.
DR   InterPro; IPR016152; PTrfase/Anion_transptr.
DR   InterPro; IPR002178; PTS_EIIA_type-2_dom.
DR   InterPro; IPR006320; PTS_Nitro_regul.
DR   Pfam; PF00359; PTS_EIIA_2; 1.
DR   SUPFAM; SSF55804; SSF55804; 1.
DR   TIGRFAMs; TIGR01419; nitro_reg_IIA; 1.
DR   PROSITE; PS51094; PTS_EIIA_TYPE_2; 1.
DR   PROSITE; PS00372; PTS_EIIA_TYPE_2_HIS; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Kinase; Reference proteome; Transferase.
FT   CHAIN           1..154
FT                   /note="Nitrogen regulatory protein"
FT                   /id="PRO_0000287733"
FT   DOMAIN          6..150
FT                   /note="PTS EIIA type-2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00417"
FT   ACT_SITE        68
FT                   /note="Tele-phosphohistidine intermediate"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00417"
SQ   SEQUENCE   154 AA;  16730 MW;  A283E657168B294E CRC64;
     MIRLEQILTP GRSLVNVPGG SKKRVLEQIA NLVARELPEF DAQTIFENLV AREKLGSTGF
     GNGIAIPHCR LSGCQSPISA VLHLDAPVDF DALDGAPVDL LFVLLVPEAA TEEHLELLRQ
     IAAMLDRADV RDRLRSAPTA EALYQIVVDV QNGQ