ID   PTSN_PSEPU              Reviewed;          89 AA.
AC   P33670;
DT   01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1994, sequence version 1.
DT   03-AUG-2022, entry version 74.
DE   RecName: Full=Nitrogen regulatory protein;
DE   AltName: Full=Enzyme IIA-NTR;
DE   Includes:
DE     RecName: Full=Phosphotransferase enzyme IIA component;
DE     AltName: Full=PTS system EIIA component;
DE   Flags: Fragment;
GN   Name=ptsN;
OS   Pseudomonas putida (Arthrobacter siderocapsulatus).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=303;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=TN2100;
RX   PubMed=2695395; DOI=10.1016/0378-1119(89)90474-5;
RA   Inouye S., Yamada M., Nakazawa A., Nakazawa T.;
RT   "Cloning and sequence analysis of the ntrA (rpoN) gene of Pseudomonas
RT   putida.";
RL   Gene 85:145-152(1989).
CC   -!- FUNCTION: Seems to have a role in regulating nitrogen assimilation.
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm.
CC   -!- DOMAIN: The PTS EIIA type-2 domain may serve a regulatory function,
CC       through its phosphorylation activity.
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DR   EMBL; M24916; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   AlphaFoldDB; P33670; -.
DR   SMR; P33670; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0016301; F:kinase activity; IEA:UniProtKB-KW.
DR   GO; GO:0016310; P:phosphorylation; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.930.10; -; 1.
DR   InterPro; IPR016152; PTrfase/Anion_transptr.
DR   InterPro; IPR002178; PTS_EIIA_type-2_dom.
DR   Pfam; PF00359; PTS_EIIA_2; 1.
DR   SUPFAM; SSF55804; SSF55804; 1.
DR   PROSITE; PS51094; PTS_EIIA_TYPE_2; 1.
DR   PROSITE; PS00372; PTS_EIIA_TYPE_2_HIS; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Kinase; Transferase.
FT   CHAIN           1..>89
FT                   /note="Nitrogen regulatory protein"
FT                   /id="PRO_0000186696"
FT   DOMAIN          6..>89
FT                   /note="PTS EIIA type-2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00417"
FT   ACT_SITE        68
FT                   /note="Tele-phosphohistidine intermediate"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00417"
FT   NON_TER         89
SQ   SEQUENCE   89 AA;  9488 MW;  8CAA4C53914E1DD3 CRC64;
     MIRLETILTP GRSLVNVPGG SKKRALEKVA TVIADQVPEL EMQDVFEKLV AREKLGSTGF
     GNGIAIPHCR LEGSSAPVSA LLHLEAPID