ID   PTSN_SHIFL              Reviewed;         163 AA.
AC   P69815; P31222;
DT   10-MAY-2005, integrated into UniProtKB/Swiss-Prot.
DT   10-MAY-2005, sequence version 1.
DT   03-AUG-2022, entry version 106.
DE   RecName: Full=Nitrogen regulatory protein;
DE   AltName: Full=Enzyme IIA-NTR;
DE   Includes:
DE     RecName: Full=Phosphotransferase enzyme IIA component;
DE     AltName: Full=PTS system EIIA component;
GN   Name=ptsN; Synonyms=rpoP; OrderedLocusNames=SF3244, S3462;
OS   Shigella flexneri.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Shigella.
OX   NCBI_TaxID=623;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=301 / Serotype 2a;
RX   PubMed=12384590; DOI=10.1093/nar/gkf566;
RA   Jin Q., Yuan Z., Xu J., Wang Y., Shen Y., Lu W., Wang J., Liu H., Yang J.,
RA   Yang F., Zhang X., Zhang J., Yang G., Wu H., Qu D., Dong J., Sun L.,
RA   Xue Y., Zhao A., Gao Y., Zhu J., Kan B., Ding K., Chen S., Cheng H.,
RA   Yao Z., He B., Chen R., Ma D., Qiang B., Wen Y., Hou Y., Yu J.;
RT   "Genome sequence of Shigella flexneri 2a: insights into pathogenicity
RT   through comparison with genomes of Escherichia coli K12 and O157.";
RL   Nucleic Acids Res. 30:4432-4441(2002).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700930 / 2457T / Serotype 2a;
RX   PubMed=12704152; DOI=10.1128/iai.71.5.2775-2786.2003;
RA   Wei J., Goldberg M.B., Burland V., Venkatesan M.M., Deng W., Fournier G.,
RA   Mayhew G.F., Plunkett G. III, Rose D.J., Darling A., Mau B., Perna N.T.,
RA   Payne S.M., Runyen-Janecky L.J., Zhou S., Schwartz D.C., Blattner F.R.;
RT   "Complete genome sequence and comparative genomics of Shigella flexneri
RT   serotype 2a strain 2457T.";
RL   Infect. Immun. 71:2775-2786(2003).
CC   -!- FUNCTION: Seems to have a role in regulating nitrogen assimilation.
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- DOMAIN: The PTS EIIA type-2 domain may serve as a regulatory function,
CC       through its phosphorylation activity.
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DR   EMBL; AE005674; AAN44710.2; -; Genomic_DNA.
DR   EMBL; AE014073; AAP18524.1; -; Genomic_DNA.
DR   RefSeq; NP_709003.2; NC_004337.2.
DR   RefSeq; WP_000183676.1; NZ_WPGW01000004.1.
DR   AlphaFoldDB; P69815; -.
DR   BMRB; P69815; -.
DR   SMR; P69815; -.
DR   STRING; 198214.SF3244; -.
DR   EnsemblBacteria; AAN44710; AAN44710; SF3244.
DR   EnsemblBacteria; AAP18524; AAP18524; S3462.
DR   GeneID; 1027089; -.
DR   GeneID; 67415962; -.
DR   KEGG; sfl:SF3244; -.
DR   KEGG; sfx:S3462; -.
DR   PATRIC; fig|198214.7.peg.3845; -.
DR   HOGENOM; CLU_072531_5_2_6; -.
DR   OMA; FDAPDEQ; -.
DR   OrthoDB; 1810962at2; -.
DR   Proteomes; UP000001006; Chromosome.
DR   Proteomes; UP000002673; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0016301; F:kinase activity; IEA:UniProtKB-KW.
DR   GO; GO:0008982; F:protein-N(PI)-phosphohistidine-sugar phosphotransferase activity; IEA:InterPro.
DR   GO; GO:0009401; P:phosphoenolpyruvate-dependent sugar phosphotransferase system; IEA:InterPro.
DR   GO; GO:0016310; P:phosphorylation; IEA:UniProtKB-KW.
DR   CDD; cd00211; PTS_IIA_fru; 1.
DR   Gene3D; 3.40.930.10; -; 1.
DR   InterPro; IPR016152; PTrfase/Anion_transptr.
DR   InterPro; IPR002178; PTS_EIIA_type-2_dom.
DR   InterPro; IPR006320; PTS_Nitro_regul.
DR   Pfam; PF00359; PTS_EIIA_2; 1.
DR   SUPFAM; SSF55804; SSF55804; 1.
DR   TIGRFAMs; TIGR01419; nitro_reg_IIA; 1.
DR   PROSITE; PS51094; PTS_EIIA_TYPE_2; 1.
DR   PROSITE; PS00372; PTS_EIIA_TYPE_2_HIS; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Kinase; Reference proteome; Transferase.
FT   CHAIN           1..163
FT                   /note="Nitrogen regulatory protein"
FT                   /id="PRO_0000186697"
FT   DOMAIN          12..156
FT                   /note="PTS EIIA type-2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00417"
FT   ACT_SITE        73
FT                   /note="Tele-phosphohistidine intermediate"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00417"
SQ   SEQUENCE   163 AA;  17960 MW;  57240CF2C550AC80 CRC64;
     MTNNDTTLQL SSVLNRECTR SRVHCQSKKR ALEIISELAA KQLSLPPQVV FEAILTREKM
     GSTGIGNGIA IPHGKLEEDT LRAVGVFVQL ETPIAFDAID NQPVDLLFAL LVPADQTKTH
     LHTLSLVAKR LADKTICRRL RAAQSDEELY QIITDTEGTP DEA