ID   PTSO_ECO57              Reviewed;          90 AA.
AC   P0A9N2; P33996;
DT   19-JUL-2005, integrated into UniProtKB/Swiss-Prot.
DT   19-JUL-2005, sequence version 1.
DT   03-AUG-2022, entry version 108.
DE   RecName: Full=Phosphocarrier protein NPr;
DE   AltName: Full=Nitrogen-related HPr;
GN   Name=ptsO; OrderedLocusNames=Z4569, ECs4085;
OS   Escherichia coli O157:H7.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83334;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=O157:H7 / EDL933 / ATCC 700927 / EHEC;
RX   PubMed=11206551; DOI=10.1038/35054089;
RA   Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J.,
RA   Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G.,
RA   Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J.,
RA   Davis N.W., Lim A., Dimalanta E.T., Potamousis K., Apodaca J.,
RA   Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A.,
RA   Blattner F.R.;
RT   "Genome sequence of enterohaemorrhagic Escherichia coli O157:H7.";
RL   Nature 409:529-533(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=O157:H7 / Sakai / RIMD 0509952 / EHEC;
RX   PubMed=11258796; DOI=10.1093/dnares/8.1.11;
RA   Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K.,
RA   Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T.,
RA   Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T., Kuhara S.,
RA   Shiba T., Hattori M., Shinagawa H.;
RT   "Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and
RT   genomic comparison with a laboratory strain K-12.";
RL   DNA Res. 8:11-22(2001).
CC   -!- FUNCTION: Component of the phosphoenolpyruvate-dependent nitrogen-
CC       metabolic phosphotransferase system (nitrogen-metabolic PTS), that
CC       seems to be involved in regulating nitrogen metabolism. The phosphoryl
CC       group from phosphoenolpyruvate (PEP) is transferred to the phosphoryl
CC       carrier protein NPr by enzyme I-Ntr. Phospho-NPr then transfers it to
CC       EIIA-Ntr. Could function in the transcriptional regulation of sigma-54
CC       dependent operons in conjunction with the NPr (PtsO) and EIIA-Ntr
CC       (PtsN) proteins. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the HPr family. {ECO:0000305}.
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DR   EMBL; AE005174; AAG58340.1; -; Genomic_DNA.
DR   EMBL; BA000007; BAB37508.1; -; Genomic_DNA.
DR   PIR; E91139; E91139.
DR   PIR; H85984; H85984.
DR   RefSeq; NP_312112.1; NC_002695.1.
DR   RefSeq; WP_000216791.1; NZ_SWKA01000005.1.
DR   AlphaFoldDB; P0A9N2; -.
DR   SMR; P0A9N2; -.
DR   STRING; 155864.EDL933_4434; -.
DR   EnsemblBacteria; AAG58340; AAG58340; Z4569.
DR   EnsemblBacteria; BAB37508; BAB37508; ECs_4085.
DR   GeneID; 66672892; -.
DR   GeneID; 916066; -.
DR   KEGG; ece:Z4569; -.
DR   KEGG; ecs:ECs_4085; -.
DR   PATRIC; fig|386585.9.peg.4264; -.
DR   eggNOG; COG1925; Bacteria.
DR   HOGENOM; CLU_136230_1_3_6; -.
DR   OMA; HARPAMM; -.
DR   Proteomes; UP000000558; Chromosome.
DR   Proteomes; UP000002519; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0009401; P:phosphoenolpyruvate-dependent sugar phosphotransferase system; IEA:UniProtKB-KW.
DR   CDD; cd00367; PTS-HPr_like; 1.
DR   Gene3D; 3.30.1340.10; -; 1.
DR   InterPro; IPR000032; HPr-like.
DR   InterPro; IPR035895; HPr-like_sf.
DR   InterPro; IPR001020; PTS_HPr_His_P_site.
DR   InterPro; IPR002114; PTS_HPr_Ser_P_site.
DR   Pfam; PF00381; PTS-HPr; 1.
DR   PRINTS; PR00107; PHOSPHOCPHPR.
DR   SUPFAM; SSF55594; SSF55594; 1.
DR   TIGRFAMs; TIGR01003; PTS_HPr_family; 1.
DR   PROSITE; PS51350; PTS_HPR_DOM; 1.
DR   PROSITE; PS00369; PTS_HPR_HIS; 1.
DR   PROSITE; PS00589; PTS_HPR_SER; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Phosphotransferase system; Reference proteome.
FT   CHAIN           1..90
FT                   /note="Phosphocarrier protein NPr"
FT                   /id="PRO_0000107891"
FT   DOMAIN          2..90
FT                   /note="HPr"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00681"
FT   ACT_SITE        16
FT                   /note="Pros-phosphohistidine intermediate"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00681"
SQ   SEQUENCE   90 AA;  9810 MW;  12A8EFEB514F6015 CRC64;
     MTVKQTVEIT NKLGMHARPA MKLFELMQGF DAEVLLRNDE GTEAEANSVI ALLMLDSAKG
     RQIEVEATGP QEEEALAAVI ALFNSGFDED