PTSO_ECOLI
ID PTSO_ECOLI Reviewed; 90 AA.
AC P0A9N0; P33996; Q2M906;
DT 19-JUL-2005, integrated into UniProtKB/Swiss-Prot.
DT 19-JUL-2005, sequence version 1.
DT 03-AUG-2022, entry version 123.
DE RecName: Full=Phosphocarrier protein NPr;
DE AltName: Full=Nitrogen-related HPr;
GN Name=ptsO; Synonyms=npr, rpoR, yhbK; OrderedLocusNames=b3206, JW3173;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND CHARACTERIZATION.
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=7876255; DOI=10.1074/jbc.270.9.4822;
RA Powell B.S., Court D.L., Inada T., Nakamura Y., Michotey V., Cui X.,
RA Reizer A., Saier M.H. Jr., Reizer J.;
RT "Novel proteins of the phosphotransferase system encoded within the rpoN
RT operon of Escherichia coli. Enzyme IIANtr affects growth on organic
RT nitrogen and the conditional lethality of an erats mutant.";
RL J. Biol. Chem. 270:4822-4839(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=K12;
RX PubMed=8025669; DOI=10.1099/13500872-140-5-1035;
RA Jones D.H.A., Franklin C.F.H., Thomas C.M.;
RT "Molecular analysis of the operon which encodes the RNA polymerase sigma
RT factor sigma 54 of Escherichia coli.";
RL Microbiology 140:1035-1043(1994).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RA Plunkett G. III;
RL Submitted (DEC-1994) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
CC -!- FUNCTION: Component of the phosphoenolpyruvate-dependent nitrogen-
CC metabolic phosphotransferase system (nitrogen-metabolic PTS), that
CC seems to be involved in regulating nitrogen metabolism. The phosphoryl
CC group from phosphoenolpyruvate (PEP) is transferred to the phosphoryl
CC carrier protein NPr by enzyme I-Ntr. Phospho-NPr then transfers it to
CC EIIA-Ntr. Could function in the transcriptional regulation of sigma-54
CC dependent operons in conjunction with the NPr (PtsO) and EIIA-Ntr
CC (PtsN) proteins.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC -!- MISCELLANEOUS: Enzyme I of the sugar PTS has been shown in
CC PubMed:7876255 to phosphorylate NPr of the nitrogen-metabolic PTS,
CC though much less efficiently than it does HPr. This process may link
CC carbon and nitrogen assimilation.
CC -!- SIMILARITY: Belongs to the HPr family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; U12684; AAB60167.1; -; Genomic_DNA.
DR EMBL; Z27094; CAA81621.1; -; Genomic_DNA.
DR EMBL; U18997; AAA58008.1; -; Genomic_DNA.
DR EMBL; U00096; AAC76238.1; -; Genomic_DNA.
DR EMBL; AP009048; BAE77250.1; -; Genomic_DNA.
DR PIR; I76722; I76722.
DR RefSeq; NP_417673.1; NC_000913.3.
DR RefSeq; WP_000216791.1; NZ_STEB01000012.1.
DR PDB; 5T17; NMR; -; A=1-85.
DR PDB; 5T1N; NMR; -; A=1-85.
DR PDB; 5T1O; NMR; -; A=1-85.
DR PDBsum; 5T17; -.
DR PDBsum; 5T1N; -.
DR PDBsum; 5T1O; -.
DR AlphaFoldDB; P0A9N0; -.
DR SASBDB; P0A9N0; -.
DR SMR; P0A9N0; -.
DR BioGRID; 4261609; 13.
DR STRING; 511145.b3206; -.
DR jPOST; P0A9N0; -.
DR PaxDb; P0A9N0; -.
DR PRIDE; P0A9N0; -.
DR EnsemblBacteria; AAC76238; AAC76238; b3206.
DR EnsemblBacteria; BAE77250; BAE77250; BAE77250.
DR GeneID; 66672892; -.
DR GeneID; 947914; -.
DR KEGG; ecj:JW3173; -.
DR KEGG; eco:b3206; -.
DR PATRIC; fig|1411691.4.peg.3525; -.
DR EchoBASE; EB2067; -.
DR eggNOG; COG1925; Bacteria.
DR HOGENOM; CLU_136230_1_3_6; -.
DR InParanoid; P0A9N0; -.
DR OMA; HARPAMM; -.
DR PhylomeDB; P0A9N0; -.
DR BioCyc; EcoCyc:EG12147-MON; -.
DR PRO; PR:P0A9N0; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0016772; F:transferase activity, transferring phosphorus-containing groups; IDA:EcoCyc.
DR GO; GO:0009401; P:phosphoenolpyruvate-dependent sugar phosphotransferase system; IEA:UniProtKB-KW.
DR GO; GO:0006468; P:protein phosphorylation; IDA:EcoCyc.
DR GO; GO:0010243; P:response to organonitrogen compound; IDA:EcoCyc.
DR CDD; cd00367; PTS-HPr_like; 1.
DR Gene3D; 3.30.1340.10; -; 1.
DR InterPro; IPR000032; HPr-like.
DR InterPro; IPR035895; HPr-like_sf.
DR InterPro; IPR001020; PTS_HPr_His_P_site.
DR InterPro; IPR002114; PTS_HPr_Ser_P_site.
DR Pfam; PF00381; PTS-HPr; 1.
DR PRINTS; PR00107; PHOSPHOCPHPR.
DR SUPFAM; SSF55594; SSF55594; 1.
DR TIGRFAMs; TIGR01003; PTS_HPr_family; 1.
DR PROSITE; PS51350; PTS_HPR_DOM; 1.
DR PROSITE; PS00369; PTS_HPR_HIS; 1.
DR PROSITE; PS00589; PTS_HPR_SER; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cytoplasm; Phosphotransferase system; Reference proteome.
FT CHAIN 1..90
FT /note="Phosphocarrier protein NPr"
FT /id="PRO_0000107890"
FT DOMAIN 2..90
FT /note="HPr"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00681"
FT ACT_SITE 16
FT /note="Pros-phosphohistidine intermediate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00681"
FT STRAND 3..8
FT /evidence="ECO:0007829|PDB:5T17"
FT STRAND 13..15
FT /evidence="ECO:0007829|PDB:5T1N"
FT HELIX 18..27
FT /evidence="ECO:0007829|PDB:5T17"
FT STRAND 35..38
FT /evidence="ECO:0007829|PDB:5T17"
FT TURN 39..41
FT /evidence="ECO:0007829|PDB:5T17"
FT STRAND 42..45
FT /evidence="ECO:0007829|PDB:5T17"
FT HELIX 49..56
FT /evidence="ECO:0007829|PDB:5T17"
FT TURN 57..60
FT /evidence="ECO:0007829|PDB:5T17"
FT STRAND 62..67
FT /evidence="ECO:0007829|PDB:5T17"
FT HELIX 72..83
FT /evidence="ECO:0007829|PDB:5T17"
SQ SEQUENCE 90 AA; 9810 MW; 12A8EFEB514F6015 CRC64;
MTVKQTVEIT NKLGMHARPA MKLFELMQGF DAEVLLRNDE GTEAEANSVI ALLMLDSAKG
RQIEVEATGP QEEEALAAVI ALFNSGFDED
