ID   PTSO_PROMH              Reviewed;          90 AA.
AC   Q9ZA86; B4EX43;
DT   27-APR-2001, integrated into UniProtKB/Swiss-Prot.
DT   20-JAN-2009, sequence version 2.
DT   25-MAY-2022, entry version 124.
DE   RecName: Full=Phosphocarrier protein NPr;
DE   AltName: Full=Nitrogen-related HPr;
GN   Name=ptsO; OrderedLocusNames=PMI3644;
OS   Proteus mirabilis (strain HI4320).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Morganellaceae; Proteus.
OX   NCBI_TaxID=529507;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=10206698; DOI=10.1099/13500872-145-1-185;
RA   Zhao H., Li X., Johnson D.E., Mobley H.L.T.;
RT   "Identification of protease and rpoN-associated genes of uropathogenic
RT   Proteus mirabilis by negative selection in a mouse model of ascending
RT   urinary tract infection.";
RL   Microbiology 145:185-195(1999).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=HI4320;
RX   PubMed=18375554; DOI=10.1128/jb.01981-07;
RA   Pearson M.M., Sebaihia M., Churcher C., Quail M.A., Seshasayee A.S.,
RA   Luscombe N.M., Abdellah Z., Arrosmith C., Atkin B., Chillingworth T.,
RA   Hauser H., Jagels K., Moule S., Mungall K., Norbertczak H.,
RA   Rabbinowitsch E., Walker D., Whithead S., Thomson N.R., Rather P.N.,
RA   Parkhill J., Mobley H.L.T.;
RT   "Complete genome sequence of uropathogenic Proteus mirabilis, a master of
RT   both adherence and motility.";
RL   J. Bacteriol. 190:4027-4037(2008).
CC   -!- FUNCTION: Component of the phosphoenolpyruvate-dependent nitrogen-
CC       metabolic phosphotransferase system (nitrogen-metabolic PTS), that
CC       seems to be involved in regulating nitrogen metabolism. The phosphoryl
CC       group from phosphoenolpyruvate (PEP) is transferred to the phosphoryl
CC       carrier protein NPr by enzyme I-Ntr. Phospho-NPr then transfers it to
CC       EIIA-Ntr. Could function in the transcriptional regulation of sigma-54
CC       dependent operons in conjunction with the NPr (PtsO) and EIIA-Ntr
CC       (PtsN) proteins. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the HPr family. {ECO:0000305}.
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DR   EMBL; AF088980; AAC64576.1; -; Genomic_DNA.
DR   EMBL; AM942759; CAR47115.1; -; Genomic_DNA.
DR   RefSeq; WP_004245309.1; NC_010554.1.
DR   AlphaFoldDB; Q9ZA86; -.
DR   SMR; Q9ZA86; -.
DR   STRING; 529507.PMI3644; -.
DR   EnsemblBacteria; CAR47115; CAR47115; PMI3644.
DR   GeneID; 6800963; -.
DR   KEGG; pmr:PMI3644; -.
DR   eggNOG; COG1925; Bacteria.
DR   HOGENOM; CLU_136230_1_3_6; -.
DR   OMA; HARPAMM; -.
DR   Proteomes; UP000008319; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0009401; P:phosphoenolpyruvate-dependent sugar phosphotransferase system; IEA:UniProtKB-KW.
DR   CDD; cd00367; PTS-HPr_like; 1.
DR   Gene3D; 3.30.1340.10; -; 1.
DR   InterPro; IPR000032; HPr-like.
DR   InterPro; IPR035895; HPr-like_sf.
DR   InterPro; IPR001020; PTS_HPr_His_P_site.
DR   InterPro; IPR002114; PTS_HPr_Ser_P_site.
DR   Pfam; PF00381; PTS-HPr; 1.
DR   PRINTS; PR00107; PHOSPHOCPHPR.
DR   SUPFAM; SSF55594; SSF55594; 1.
DR   TIGRFAMs; TIGR01003; PTS_HPr_family; 1.
DR   PROSITE; PS51350; PTS_HPR_DOM; 1.
DR   PROSITE; PS00369; PTS_HPR_HIS; 1.
DR   PROSITE; PS00589; PTS_HPR_SER; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Phosphotransferase system; Reference proteome.
FT   CHAIN           1..90
FT                   /note="Phosphocarrier protein NPr"
FT                   /id="PRO_0000107894"
FT   DOMAIN          2..90
FT                   /note="HPr"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00681"
FT   ACT_SITE        16
FT                   /note="Pros-phosphohistidine intermediate"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00681"
FT   CONFLICT        28
FT                   /note="N -> H (in Ref. 1; AAC64576)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        65..66
FT                   /note="IE -> MK (in Ref. 1; AAC64576)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        75
FT                   /note="A -> T (in Ref. 1; AAC64576)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   90 AA;  10014 MW;  8E13F66943C5A2FF CRC64;
     MTQYRRVAIK NRLGMHARPA MKLFDLVNTF QSTVTLRNHE GVEAQADSVI AMLMLDSEQG
     SHIDIEASGC DEKEAIDAII ALFESGFDED