ID   PTSP_BOMMO              Reviewed;         274 AA.
AC   P82003; Q95YF7;
DT   26-SEP-2001, integrated into UniProtKB/Swiss-Prot.
DT   20-DEC-2005, sequence version 2.
DT   25-MAY-2022, entry version 59.
DE   RecName: Full=Prothoracicostatic peptide;
DE   AltName: Full=Bom-PTSP;
DE   Flags: Precursor;
OS   Bombyx mori (Silk moth).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Lepidoptera; Glossata; Ditrysia; Bombycoidea;
OC   Bombycidae; Bombycinae; Bombyx.
OX   NCBI_TaxID=7091;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Brain;
RA   Hua Y.-J., Roller L., Kataoka H., Tanaka Y.;
RT   "cDNA of Bombyx prothoracicostatic peptide (PTSP) encodes a unique peptide
RT   family of -WXXXXXXW-amide.";
RL   Submitted (OCT-2001) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   PROTEIN SEQUENCE OF 68-76, FUNCTION, MASS SPECTROMETRY, AND AMIDATION AT
RP   TRP-76; TRP-100; TRP-145; TRP-157 AND TRP-181.
RC   STRAIN=C145 X N140; TISSUE=Brain;
RX   PubMed=10531308; DOI=10.1074/jbc.274.44.31169;
RA   Hua Y.-J., Tanaka Y., Nakamura K., Sakakibara M., Nagata S., Kataoka H.;
RT   "Identification of a prothoracicostatic peptide in the larval brain of the
RT   silkworm, Bombyx mori.";
RL   J. Biol. Chem. 274:31169-31173(1999).
RN   [3]
RP   ERRATUM OF PUBMED:10531308.
RA   Hua Y.-J., Tanaka Y., Nakamura K., Sakakibara M., Nagata S., Kataoka H.;
RL   J. Biol. Chem. 275:9892-9892(2000).
RN   [4]
RP   FUNCTION.
RX   PubMed=15274176; DOI=10.1002/arch.20005;
RA   Liu X., Tanaka Y., Song Q., Xu B., Hua Y.-J.;
RT   "Bombyx mori prothoracicostatic peptide inhibits ecdysteroidogenesis in
RT   vivo.";
RL   Arch. Insect Biochem. Physiol. 56:155-161(2004).
CC   -!- FUNCTION: Inhibits ecdysteroid biosynthesis in the prothoracic gland of
CC       fifth instar larvae, with maximum inhibition during the spinning stage.
CC       When administered to day 8 fifth instar larvae it produces a
CC       significant delay in the commencement spinning behavior.
CC       {ECO:0000269|PubMed:10531308, ECO:0000269|PubMed:15274176}.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- MASS SPECTROMETRY: Mass=1089.78; Method=MALDI;
CC       Evidence={ECO:0000269|PubMed:10531308};
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DR   EMBL; AB073553; BAB70681.1; -; mRNA.
DR   RefSeq; NP_001036890.1; NM_001043425.1.
DR   AlphaFoldDB; P82003; -.
DR   PRIDE; P82003; -.
DR   GeneID; 692434; -.
DR   KEGG; bmor:692434; -.
DR   CTD; 692434; -.
DR   OrthoDB; 1344573at2759; -.
DR   Proteomes; UP000005204; Unassembled WGS sequence.
DR   GO; GO:0005615; C:extracellular space; IDA:UniProtKB.
DR   GO; GO:0005179; F:hormone activity; IEA:UniProtKB-KW.
DR   GO; GO:0002168; P:instar larval development; IDA:UniProtKB.
PE   1: Evidence at protein level;
KW   Amidation; Cleavage on pair of basic residues; Direct protein sequencing;
KW   Hormone; Reference proteome; Secreted; Signal.
FT   SIGNAL          1..19
FT                   /evidence="ECO:0000255"
FT   PROPEP          20..67
FT                   /evidence="ECO:0000305|PubMed:10531308"
FT                   /id="PRO_0000045250"
FT   PEPTIDE         68..76
FT                   /note="Prothoracicostatic peptide"
FT                   /id="PRO_0000045251"
FT   PROPEP          77..91
FT                   /evidence="ECO:0000305"
FT                   /id="PRO_0000045252"
FT   PEPTIDE         92..100
FT                   /note="Prothoracicostatic peptide"
FT                   /evidence="ECO:0000305"
FT                   /id="PRO_0000045253"
FT   PROPEP          101..136
FT                   /evidence="ECO:0000305"
FT                   /id="PRO_0000045254"
FT   PEPTIDE         137..145
FT                   /note="Prothoracicostatic peptide"
FT                   /evidence="ECO:0000305"
FT                   /id="PRO_0000045255"
FT   PROPEP          146..148
FT                   /evidence="ECO:0000305"
FT                   /id="PRO_0000045256"
FT   PEPTIDE         149..157
FT                   /note="Prothoracicostatic peptide"
FT                   /evidence="ECO:0000305"
FT                   /id="PRO_0000045257"
FT   PROPEP          158..172
FT                   /evidence="ECO:0000305"
FT                   /id="PRO_0000045258"
FT   PEPTIDE         173..181
FT                   /note="Prothoracicostatic peptide"
FT                   /evidence="ECO:0000305"
FT                   /id="PRO_0000045259"
FT   PROPEP          182..274
FT                   /evidence="ECO:0000305"
FT                   /id="PRO_0000045260"
FT   MOD_RES         76
FT                   /note="Tryptophan amide"
FT                   /evidence="ECO:0000269|PubMed:10531308"
FT   MOD_RES         100
FT                   /note="Tryptophan amide"
FT                   /evidence="ECO:0000269|PubMed:10531308"
FT   MOD_RES         145
FT                   /note="Tryptophan amide"
FT                   /evidence="ECO:0000269|PubMed:10531308"
FT   MOD_RES         157
FT                   /note="Tryptophan amide"
FT                   /evidence="ECO:0000269|PubMed:10531308"
FT   MOD_RES         181
FT                   /note="Tryptophan amide"
FT                   /evidence="ECO:0000269|PubMed:10531308"
SQ   SEQUENCE   274 AA;  31660 MW;  ACC33700E01D389C CRC64;
     MRWCLFALWV FGVATVVTAA EEPHHDAAPQ TDNEVDLTED DKRAWSSLHS GWAKRAWQDM
     SSAWGKRAWQ DLNSAWGKRG WQDLNSAWGK RAWQDLNSAW GKRGWQDLNS AWGKRDDDEA
     MEKKSWQDLN SVWGKRAWQD LNSAWGKRAW QDLNSAWGKR GWNDISSVWG KRAWQDLNSA
     WGKRAWQDMS SAWGKRAPEK WAAFHGSWGK RSSIEPDYEE IDAVEQLVPY QQAPNEEHID
     APEKKAWSAL HGTWGKRPVK PMFNNEHSAT TNEA