ID   PTVO1_RAT               Reviewed;         416 AA.
AC   Q5U2W6;
DT   02-OCT-2007, integrated into UniProtKB/Swiss-Prot.
DT   07-DEC-2004, sequence version 1.
DT   03-AUG-2022, entry version 87.
DE   RecName: Full=Prostate tumor-overexpressed gene 1 protein homolog;
GN   Name=Ptov1;
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Testis;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
CC   -!- FUNCTION: May activate transcription. Required for nuclear
CC       translocation of FLOT1. Promotes cell proliferation.
CC       {ECO:0000250|UniProtKB:Q86YD1}.
CC   -!- SUBUNIT: May interact with CREBBP. Interacts with FLOT1.
CC       {ECO:0000250|UniProtKB:Q86YD1}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:Q86YD1}. Nucleus
CC       {ECO:0000250|UniProtKB:Q86YD1}. Cell membrane
CC       {ECO:0000250|UniProtKB:Q86YD1}. Cytoplasm, perinuclear region
CC       {ECO:0000250|UniProtKB:Q86YD1}. Note=Translocates from the cytoplasm to
CC       the nucleus at the onset of S-phase. Also localizes to lipid rafts.
CC       {ECO:0000250|UniProtKB:Q86YD1}.
CC   -!- PTM: Ubiquitinated by the CRL2(KLHDC2) complex, which recognizes the
CC       diglycine (Gly-Gly) at the C-terminus, leading to its degradation.
CC       Ubiquitinated by the CRL2(APPBP2) complex, which recognizes the Arg-
CC       Xaa-Xaa-Gly sequence at the C-terminus, leading to its degradation.
CC       {ECO:0000250|UniProtKB:Q86YD1}.
CC   -!- SIMILARITY: Belongs to the Mediator complex subunit 25 family. PTOV1
CC       subfamily. {ECO:0000305}.
CC   -!- CAUTION: Despite sequence similarity to MED25, to date this protein has
CC       not been identified as a component of the Mediator complex.
CC       {ECO:0000305}.
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DR   EMBL; BC085836; AAH85836.1; -; mRNA.
DR   RefSeq; NP_001008305.1; NM_001008304.1.
DR   AlphaFoldDB; Q5U2W6; -.
DR   SMR; Q5U2W6; -.
DR   STRING; 10116.ENSRNOP00000027580; -.
DR   PhosphoSitePlus; Q5U2W6; -.
DR   PaxDb; Q5U2W6; -.
DR   PRIDE; Q5U2W6; -.
DR   Ensembl; ENSRNOT00000027580; ENSRNOP00000027580; ENSRNOG00000020358.
DR   GeneID; 292888; -.
DR   KEGG; rno:292888; -.
DR   UCSC; RGD:1306977; rat.
DR   CTD; 53635; -.
DR   RGD; 1306977; Ptov1.
DR   eggNOG; ENOG502SS25; Eukaryota.
DR   GeneTree; ENSGT00940000161923; -.
DR   HOGENOM; CLU_045342_0_0_1; -.
DR   InParanoid; Q5U2W6; -.
DR   OMA; ELDCLTH; -.
DR   OrthoDB; 340324at2759; -.
DR   PhylomeDB; Q5U2W6; -.
DR   TreeFam; TF329598; -.
DR   PRO; PR:Q5U2W6; -.
DR   Proteomes; UP000002494; Chromosome 1.
DR   Bgee; ENSRNOG00000020358; Expressed in skeletal muscle tissue and 19 other tissues.
DR   Genevisible; Q5U2W6; RN.
DR   GO; GO:0005654; C:nucleoplasm; IEA:Ensembl.
DR   GO; GO:0048471; C:perinuclear region of cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005667; C:transcription regulator complex; IBA:GO_Central.
DR   GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR   Gene3D; 2.40.290.30; -; 2.
DR   InterPro; IPR045105; Med25/PTOV1.
DR   InterPro; IPR021394; Med25_PTOV.
DR   InterPro; IPR038196; Med25_PTOV_sf.
DR   PANTHER; PTHR12433; PTHR12433; 2.
DR   Pfam; PF11232; Med25; 2.
PE   2: Evidence at transcript level;
KW   Activator; Cell membrane; Cytoplasm; Membrane; Nucleus; Phosphoprotein;
KW   Reference proteome; Transcription; Transcription regulation;
KW   Ubl conjugation.
FT   CHAIN           1..416
FT                   /note="Prostate tumor-overexpressed gene 1 protein homolog"
FT                   /id="PRO_0000304967"
FT   REGION          1..53
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          184..416
FT                   /note="Interaction with FLOT1"
FT                   /evidence="ECO:0000250"
FT   MOD_RES         53
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:Q91VU8"
SQ   SEQUENCE   416 AA;  46849 MW;  BAEAE4B65CB58A10 CRC64;
     MVRPRRAPHR SGAGGPLGGR GRPPRPLVVR AVRSRSWPAG PRGPQPPRIR ARSAPPMEGA
     RVFGALGPIG PSSPGLTLGG LAVNEHRLSN KLLAWSGVLE WQEKRRPFSD SAAKLKRTLP
     CQAYVNQGEN LETDQWPQKL IMQLIPQQLL TTLGPLFRNS QLAQFHFTNR DCDSLKGLCR
     IMGNGFAGCM LFPHISPCEV RVLMLLYSSK KKIFMGLIPY DQSGFVNAIR QVITTRKQAV
     GPGGVHSGPV QIVNNKFLAW SGVMEWQEPR PEPNSRSKRW LPSHVYVNQG EILRTDQWPR
     RLFMQLIPQQ LLTTLVPLFR NSRLVQFHFT KDMETLKSLC RIMDNGFAGC VHFSYKASCE
     VRVLMLLYSS EKKIFIGLIP HDQSNFVNGI RRVIANQQQV LQRSLEQEQQ QRGMGG