ID   PUAC_STRAD              Reviewed;         199 AA.
AC   P13249;
DT   01-JAN-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1996, sequence version 2.
DT   03-AUG-2022, entry version 83.
DE   RecName: Full=Puromycin N-acetyltransferase;
DE            EC=2.3.-.-;
GN   Name=pac;
OS   Streptomyces alboniger.
OC   Bacteria; Actinobacteria; Streptomycetales; Streptomycetaceae;
OC   Streptomyces; Streptomyces aurantiacus group.
OX   NCBI_TaxID=132473;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 12461 / DSM 40043 / JCM 4309 / NBRC 12738 / NCIMB 13007 / NRRL
RC   B-2403;
RX   PubMed=2676728; DOI=10.1016/0378-1119(89)90220-5;
RA   Lacalle R.A., Pulido D., Vara J., Zalacain M., Jimenez A.;
RT   "Molecular analysis of the pac gene encoding a puromycin N-acetyl
RT   transferase from Streptomyces alboniger.";
RL   Gene 79:375-380(1989).
CC   -!- FUNCTION: Detoxification of puromycin.
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DR   EMBL; M25346; AAA64928.1; -; Genomic_DNA.
DR   PIR; JU0052; JU0052.
DR   RefSeq; WP_055528321.1; NZ_CP023695.1.
DR   PDB; 7K09; X-ray; 2.31 A; A/B/C/D/E/F=2-199.
DR   PDB; 7K0A; X-ray; 2.00 A; A/B=2-199.
DR   PDBsum; 7K09; -.
DR   PDBsum; 7K0A; -.
DR   AlphaFoldDB; P13249; -.
DR   SMR; P13249; -.
DR   KEGG; ag:AAA64928; -.
DR   BioCyc; MetaCyc:MON-13983; -.
DR   GO; GO:0008080; F:N-acetyltransferase activity; IEA:InterPro.
DR   GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR   InterPro; IPR016181; Acyl_CoA_acyltransferase.
DR   InterPro; IPR000182; GNAT_dom.
DR   Pfam; PF00583; Acetyltransf_1; 1.
DR   SUPFAM; SSF55729; SSF55729; 1.
DR   PROSITE; PS51186; GNAT; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Acyltransferase; Antibiotic resistance; Transferase.
FT   CHAIN           1..199
FT                   /note="Puromycin N-acetyltransferase"
FT                   /id="PRO_0000068578"
FT   DOMAIN          6..198
FT                   /note="N-acetyltransferase"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00532"
FT   STRAND          8..10
FT                   /evidence="ECO:0007829|PDB:7K0A"
FT   HELIX           13..15
FT                   /evidence="ECO:0007829|PDB:7K0A"
FT   HELIX           16..26
FT                   /evidence="ECO:0007829|PDB:7K0A"
FT   TURN            27..29
FT                   /evidence="ECO:0007829|PDB:7K0A"
FT   HELIX           31..36
FT                   /evidence="ECO:0007829|PDB:7K0A"
FT   HELIX           42..56
FT                   /evidence="ECO:0007829|PDB:7K0A"
FT   HELIX           58..61
FT                   /evidence="ECO:0007829|PDB:7K0A"
FT   STRAND          62..67
FT                   /evidence="ECO:0007829|PDB:7K0A"
FT   HELIX           68..70
FT                   /evidence="ECO:0007829|PDB:7K0A"
FT   STRAND          72..77
FT                   /evidence="ECO:0007829|PDB:7K0A"
FT   HELIX           84..99
FT                   /evidence="ECO:0007829|PDB:7K0A"
FT   HELIX           100..102
FT                   /evidence="ECO:0007829|PDB:7K0A"
FT   HELIX           103..113
FT                   /evidence="ECO:0007829|PDB:7K0A"
FT   HELIX           114..116
FT                   /evidence="ECO:0007829|PDB:7K0A"
FT   STRAND          123..130
FT                   /evidence="ECO:0007829|PDB:7K0A"
FT   HELIX           132..134
FT                   /evidence="ECO:0007829|PDB:7K0A"
FT   HELIX           139..154
FT                   /evidence="ECO:0007829|PDB:7K0A"
FT   STRAND          158..163
FT                   /evidence="ECO:0007829|PDB:7K0A"
FT   HELIX           165..167
FT                   /evidence="ECO:0007829|PDB:7K0A"
FT   HELIX           168..173
FT                   /evidence="ECO:0007829|PDB:7K0A"
FT   STRAND          177..182
FT                   /evidence="ECO:0007829|PDB:7K0A"
FT   STRAND          190..195
FT                   /evidence="ECO:0007829|PDB:7K0A"
SQ   SEQUENCE   199 AA;  21496 MW;  BEDA36E20304DA35 CRC64;
     MTEYKPTVRL ATRDDVPRAV RTLAAAFADY PATRHTVDPD RHIERVTELQ ELFLTRVGLD
     IGKVWVADDG AAVAVWTTPE SVEAGAVFAE IGPRMAELSG SRLAAQQQME GLLAPHRPKE
     PAWFLATVGV SPDHQGKGLG SAVVLPGVEA AERAGVPAFL ETSAPRNLPF YERLGFTVTA
     DVEVPEGPRT WCMTRKPGA