PUB10_ARATH
ID PUB10_ARATH Reviewed; 628 AA.
AC Q9C9A6; Q56ZJ4; Q9SSJ6;
DT 26-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 136.
DE RecName: Full=U-box domain-containing protein 10;
DE EC=2.3.2.27;
DE AltName: Full=Plant U-box protein 10;
DE AltName: Full=RING-type E3 ubiquitin transferase PUB10 {ECO:0000305};
GN Name=PUB10; OrderedLocusNames=At1g71020; ORFNames=F15H11.22, F23N20.1;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 419-628.
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP GENE FAMILY ORGANIZATION, AND NOMENCLATURE.
RX PubMed=11495788; DOI=10.1016/s1360-1385(01)01960-4;
RA Azevedo C., Santos-Rosa M.J., Shirasu K.;
RT "The U-box protein family in plants.";
RL Trends Plant Sci. 6:354-358(2001).
RN [6]
RP GENE FAMILY ORGANIZATION.
RX PubMed=14657406; DOI=10.1104/pp.103.029553;
RA Mudgil Y., Shiu S.-H., Stone S.L., Salt J.N., Goring D.R.;
RT "A large complement of the predicted Arabidopsis ARM repeat proteins are
RT members of the U-box E3 ubiquitin ligase family.";
RL Plant Physiol. 134:59-66(2004).
CC -!- FUNCTION: Functions as an E3 ubiquitin ligase. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine +
CC [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.;
CC EC=2.3.2.27;
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=1;
CC Comment=A number of isoforms are produced. According to EST
CC sequences.;
CC Name=1;
CC IsoId=Q9C9A6-1; Sequence=Displayed;
CC -!- SEQUENCE CAUTION:
CC Sequence=AAD55500.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=BAD94539.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AC008148; AAD55500.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AC016972; AAG51682.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE35152.1; -; Genomic_DNA.
DR EMBL; AY075626; AAL91637.1; -; mRNA.
DR EMBL; AY142062; AAM98326.1; -; mRNA.
DR EMBL; AK220971; BAD94539.1; ALT_INIT; mRNA.
DR PIR; E96734; E96734.
DR RefSeq; NP_177258.3; NM_105771.4. [Q9C9A6-1]
DR AlphaFoldDB; Q9C9A6; -.
DR SMR; Q9C9A6; -.
DR BioGRID; 28662; 12.
DR IntAct; Q9C9A6; 3.
DR STRING; 3702.AT1G71020.1; -.
DR PaxDb; Q9C9A6; -.
DR PRIDE; Q9C9A6; -.
DR EnsemblPlants; AT1G71020.1; AT1G71020.1; AT1G71020. [Q9C9A6-1]
DR GeneID; 843442; -.
DR Gramene; AT1G71020.1; AT1G71020.1; AT1G71020. [Q9C9A6-1]
DR KEGG; ath:AT1G71020; -.
DR Araport; AT1G71020; -.
DR TAIR; locus:2013990; AT1G71020.
DR eggNOG; KOG0167; Eukaryota.
DR HOGENOM; CLU_006348_5_1_1; -.
DR InParanoid; Q9C9A6; -.
DR PhylomeDB; Q9C9A6; -.
DR UniPathway; UPA00143; -.
DR PRO; PR:Q9C9A6; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9C9A6; baseline and differential.
DR Genevisible; Q9C9A6; AT.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; IDA:TAIR.
DR GO; GO:0043621; F:protein self-association; IPI:TAIR.
DR GO; GO:0004842; F:ubiquitin-protein transferase activity; IDA:TAIR.
DR GO; GO:0051865; P:protein autoubiquitination; IDA:TAIR.
DR GO; GO:0031648; P:protein destabilization; IMP:TAIR.
DR GO; GO:0016567; P:protein ubiquitination; IDA:TAIR.
DR GO; GO:2000022; P:regulation of jasmonic acid mediated signaling pathway; IMP:TAIR.
DR CDD; cd16664; RING-Ubox_PUB; 1.
DR Gene3D; 1.25.10.10; -; 2.
DR Gene3D; 3.30.40.10; -; 1.
DR InterPro; IPR011989; ARM-like.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR000225; Armadillo.
DR InterPro; IPR045210; RING-Ubox_PUB.
DR InterPro; IPR003613; Ubox_domain.
DR InterPro; IPR013083; Znf_RING/FYVE/PHD.
DR Pfam; PF00514; Arm; 3.
DR Pfam; PF04564; U-box; 1.
DR SMART; SM00185; ARM; 5.
DR SMART; SM00504; Ubox; 1.
DR SUPFAM; SSF48371; SSF48371; 1.
DR PROSITE; PS50176; ARM_REPEAT; 2.
DR PROSITE; PS51698; U_BOX; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Reference proteome; Repeat; Transferase;
KW Ubl conjugation pathway.
FT CHAIN 1..628
FT /note="U-box domain-containing protein 10"
FT /id="PRO_0000322155"
FT DOMAIN 242..316
FT /note="U-box"
FT REPEAT 373..413
FT /note="ARM 1"
FT REPEAT 415..454
FT /note="ARM 2"
FT REPEAT 456..495
FT /note="ARM 3"
FT REPEAT 497..537
FT /note="ARM 4"
FT REPEAT 539..578
FT /note="ARM 5"
SQ SEQUENCE 628 AA; 68975 MW; 329E1A595CAE8788 CRC64;
MAGGAITPDS LIGLIAEINE IPGNFGLFKK DCSDLARRVG LLTHLIEEIR DSSPPSESDA
SSSLNSHECD WWSDLVVGLQ AAKRLLSSAT SFQARESSDG AAKRISFQFQ CVTWKLEKAL
GDLTYDRYDI SDEVREQVEL ARLQLRRAMQ RYGSLNSKKF SSGLSEPMEK DASSNRKVIE
KLESIPETVH SLSDEKKFES PPPWKSSSVS LAFFLSKDGD DERLEKAVTE NSDDSQKSDN
LTIPEDFLCP ISLELMKDPA IVSTGQTYER SFIQRWIDCG NLSCPKTQQK LENFTLTPNY
VLRSLISQWC TKHNIEQPGG YMNGRTKNSD GSFRDLSGDM SAIRALVCKL SSQSIEDRRT
AVSEIRSLSK RSTDNRILIA EAGAIPVLVK LLTSDGDTET QENAVTCILN LSIYEHNKEL
IMLAGAVTSI VLVLRAGSME ARENAAATLF SLSLADENKI IIGASGAIMA LVDLLQYGSV
RGKKDAATAL FNLCIYQGNK GRAVRAGIVK PLVKMLTDSS SERMADEALT ILSVLASNQV
AKTAILRANA IPPLIDCLQK DQPRNRENAA AILLCLCKRD TEKLISIGRL GAVVPLMELS
RDGTERAKRK ANSLLELLRK SSRKLGSL
