PUB12_ARATH
ID PUB12_ARATH Reviewed; 654 AA.
AC Q9ZV31; F4IJM7; Q94C99;
DT 26-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1999, sequence version 1.
DT 25-MAY-2022, entry version 149.
DE RecName: Full=U-box domain-containing protein 12;
DE EC=2.3.2.27;
DE AltName: Full=Plant U-box protein 12;
DE AltName: Full=RING-type E3 ubiquitin transferase PUB12 {ECO:0000305};
GN Name=PUB12; OrderedLocusNames=At2g28830; ORFNames=F8N16.12;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP GENE FAMILY ORGANIZATION, AND NOMENCLATURE.
RX PubMed=11495788; DOI=10.1016/s1360-1385(01)01960-4;
RA Azevedo C., Santos-Rosa M.J., Shirasu K.;
RT "The U-box protein family in plants.";
RL Trends Plant Sci. 6:354-358(2001).
RN [6]
RP GENE FAMILY ORGANIZATION.
RX PubMed=14657406; DOI=10.1104/pp.103.029553;
RA Mudgil Y., Shiu S.-H., Stone S.L., Salt J.N., Goring D.R.;
RT "A large complement of the predicted Arabidopsis ARM repeat proteins are
RT members of the U-box E3 ubiquitin ligase family.";
RL Plant Physiol. 134:59-66(2004).
CC -!- FUNCTION: Functions as an E3 ubiquitin ligase. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine +
CC [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.;
CC EC=2.3.2.27;
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC -!- SEQUENCE CAUTION:
CC Sequence=AEC08178.2; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC005727; AAC79587.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC08178.2; ALT_SEQ; Genomic_DNA.
DR EMBL; AY035038; AAK59543.1; -; mRNA.
DR EMBL; AK226821; BAE98917.1; -; mRNA.
DR PIR; D84689; D84689.
DR RefSeq; NP_001318308.1; NM_001336190.1.
DR AlphaFoldDB; Q9ZV31; -.
DR SMR; Q9ZV31; -.
DR STRING; 3702.AT2G28830.1; -.
DR PaxDb; Q9ZV31; -.
DR PeptideAtlas; Q9ZV31; -.
DR PRIDE; Q9ZV31; -.
DR ProteomicsDB; 226068; -.
DR GeneID; 817432; -.
DR KEGG; ath:AT2G28830; -.
DR Araport; AT2G28830; -.
DR eggNOG; KOG0167; Eukaryota.
DR eggNOG; KOG0258; Eukaryota.
DR eggNOG; KOG3422; Eukaryota.
DR HOGENOM; CLU_006348_5_1_1; -.
DR InParanoid; Q9ZV31; -.
DR OrthoDB; 389393at2759; -.
DR PhylomeDB; Q9ZV31; -.
DR UniPathway; UPA00143; -.
DR PRO; PR:Q9ZV31; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q9ZV31; baseline and differential.
DR Genevisible; Q9ZV31; AT.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0004842; F:ubiquitin-protein transferase activity; IEA:InterPro.
DR GO; GO:0007166; P:cell surface receptor signaling pathway; IEA:InterPro.
DR CDD; cd16664; RING-Ubox_PUB; 1.
DR Gene3D; 1.20.930.20; -; 1.
DR Gene3D; 1.25.10.10; -; 1.
DR Gene3D; 3.30.40.10; -; 1.
DR InterPro; IPR036537; Adaptor_Cbl_N_dom_sf.
DR InterPro; IPR011989; ARM-like.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR000225; Armadillo.
DR InterPro; IPR045210; RING-Ubox_PUB.
DR InterPro; IPR003613; Ubox_domain.
DR InterPro; IPR013083; Znf_RING/FYVE/PHD.
DR Pfam; PF00514; Arm; 3.
DR Pfam; PF04564; U-box; 1.
DR SMART; SM00185; ARM; 6.
DR SMART; SM00504; Ubox; 1.
DR SUPFAM; SSF48371; SSF48371; 1.
DR PROSITE; PS50176; ARM_REPEAT; 2.
DR PROSITE; PS51698; U_BOX; 1.
PE 2: Evidence at transcript level;
KW Reference proteome; Repeat; Transferase; Ubl conjugation pathway.
FT CHAIN 1..654
FT /note="U-box domain-containing protein 12"
FT /id="PRO_0000322157"
FT DOMAIN 255..329
FT /note="U-box"
FT REPEAT 387..427
FT /note="ARM 1"
FT REPEAT 430..469
FT /note="ARM 2"
FT REPEAT 471..510
FT /note="ARM 3"
FT REPEAT 512..551
FT /note="ARM 4"
FT REPEAT 553..592
FT /note="ARM 5"
FT REGION 329..352
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 331..348
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 373
FT /note="R -> K (in Ref. 3; AAK59543)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 654 AA; 71942 MW; B8CA7C59C970715E CRC64;
MAKSEKHKLA QTLIDSINEI ASISDSVTPM KKHCANLSRR LSLLLPMLEE IRDNQESSSE
VVNALLSVKQ SLLHAKDLLS FVSHVSKIYL VLERDQVMVK FQKVTSLLEQ ALSIIPYENL
EISDELKEQV ELVLVQLRRS LGKRGGDVYD DELYKDVLSL YSGRGSVMES DMVRRVAEKL
QLMTITDLTQ ESLALLDMVS SSGGDDPGES FEKMSMVLKK IKDFVQTYNP NLDDAPLRLK
SSLPKSRDDD RDMLIPPEEF RCPISLELMT DPVIVSSGQT YERECIKKWL EGGHLTCPKT
QETLTSDIMT PNYVLRSLIA QWCESNGIEP PKRPNISQPS SKASSSSSAP DDEHNKIEEL
LLKLTSQQPE DRRSAAGEIR LLAKQNNHNR VAIAASGAIP LLVNLLTISN DSRTQEHAVT
SILNLSICQE NKGKIVYSSG AVPGIVHVLQ KGSMEARENA AATLFSLSVI DENKVTIGAA
GAIPPLVTLL SEGSQRGKKD AATALFNLCI FQGNKGKAVR AGLVPVLMRL LTEPESGMVD
ESLSILAILS SHPDGKSEVG AADAVPVLVD FIRSGSPRNK ENSAAVLVHL CSWNQQHLIE
AQKLGIMDLL IEMAENGTDR GKRKAAQLLN RFSRFNDQQK QHSGLGLEDQ ISLI
