PUB12_ORYSJ
ID PUB12_ORYSJ Reviewed; 611 AA.
AC Q5VRH9; Q5VRH8;
DT 05-OCT-2010, integrated into UniProtKB/Swiss-Prot.
DT 07-DEC-2004, sequence version 1.
DT 03-AUG-2022, entry version 111.
DE RecName: Full=U-box domain-containing protein 12;
DE EC=2.3.2.27;
DE AltName: Full=Plant U-box protein 12;
DE Short=OsPUB12;
DE AltName: Full=RING-type E3 ubiquitin transferase PUB12 {ECO:0000305};
GN Name=PUB12; OrderedLocusNames=Os06g0102700, LOC_Os06g01304;
GN ORFNames=OSJNBa0075G19.19-1;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
RN [5]
RP FUNCTION, GENE FAMILY, AND NOMENCLATURE.
RX PubMed=19825583; DOI=10.1093/mp/ssn044;
RA Zeng L.R., Park C.H., Venu R.C., Gough J., Wang G.L.;
RT "Classification, expression pattern, and E3 ligase activity assay of rice
RT U-box-containing proteins.";
RL Mol. Plant 1:800-815(2008).
CC -!- FUNCTION: Possesses E3 ubiquitin-protein ligase in vitro.
CC {ECO:0000269|PubMed:19825583}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine +
CC [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.;
CC EC=2.3.2.27;
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q5VRH9-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q5VRH9-2; Sequence=VSP_039692;
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DR EMBL; AP002842; BAD67946.1; -; Genomic_DNA.
DR EMBL; AP002842; BAD67947.1; -; Genomic_DNA.
DR EMBL; AP008212; BAF18449.2; -; Genomic_DNA.
DR EMBL; AP014962; BAS95707.1; -; Genomic_DNA.
DR EMBL; AK067611; BAG90500.1; -; mRNA.
DR EMBL; AK071080; -; NOT_ANNOTATED_CDS; mRNA.
DR RefSeq; XP_015643776.1; XM_015788290.1. [Q5VRH9-1]
DR RefSeq; XP_015643777.1; XM_015788291.1. [Q5VRH9-1]
DR AlphaFoldDB; Q5VRH9; -.
DR SMR; Q5VRH9; -.
DR STRING; 4530.OS06T0102700-01; -.
DR PaxDb; Q5VRH9; -.
DR PRIDE; Q5VRH9; -.
DR EnsemblPlants; Os06t0102700-01; Os06t0102700-01; Os06g0102700. [Q5VRH9-2]
DR GeneID; 4339839; -.
DR Gramene; Os06t0102700-01; Os06t0102700-01; Os06g0102700. [Q5VRH9-2]
DR KEGG; osa:4339839; -.
DR eggNOG; KOG0167; Eukaryota.
DR HOGENOM; CLU_006348_5_1_1; -.
DR InParanoid; Q5VRH9; -.
DR OMA; TGSMETR; -.
DR OrthoDB; 389393at2759; -.
DR UniPathway; UPA00143; -.
DR Proteomes; UP000000763; Chromosome 6.
DR Proteomes; UP000059680; Chromosome 6.
DR Genevisible; Q5VRH9; OS.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0004842; F:ubiquitin-protein transferase activity; IDA:UniProtKB.
DR GO; GO:0016567; P:protein ubiquitination; IDA:UniProtKB.
DR CDD; cd16664; RING-Ubox_PUB; 1.
DR Gene3D; 1.25.10.10; -; 1.
DR Gene3D; 3.30.40.10; -; 1.
DR InterPro; IPR011989; ARM-like.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR000225; Armadillo.
DR InterPro; IPR045210; RING-Ubox_PUB.
DR InterPro; IPR003613; Ubox_domain.
DR InterPro; IPR013083; Znf_RING/FYVE/PHD.
DR Pfam; PF00514; Arm; 3.
DR Pfam; PF04564; U-box; 1.
DR SMART; SM00185; ARM; 5.
DR SMART; SM00504; Ubox; 1.
DR SUPFAM; SSF48371; SSF48371; 1.
DR PROSITE; PS50176; ARM_REPEAT; 1.
DR PROSITE; PS51698; U_BOX; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Reference proteome; Repeat; Transferase;
KW Ubl conjugation pathway.
FT CHAIN 1..611
FT /note="U-box domain-containing protein 12"
FT /id="PRO_0000397690"
FT DOMAIN 227..301
FT /note="U-box"
FT REPEAT 355..394
FT /note="ARM 1"
FT REPEAT 396..435
FT /note="ARM 2"
FT REPEAT 437..476
FT /note="ARM 3"
FT REPEAT 478..517
FT /note="ARM 4"
FT VAR_SEQ 604..611
FT /note="DSLKGNGH -> A (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:12869764"
FT /id="VSP_039692"
FT CONFLICT 62
FT /note="L -> I (in Ref. 4; AK071080)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 611 AA; 66241 MW; F982EF57A81E376F CRC64;
MPKRVADEIA ALPEPRGPLR RPCADLSRRV RLLAPLLDHL PASSSSSSST PLADALGAAR
DLLRKTRDGS KIDQAMRGDA FLDEFAGVNR QIHLALDALP YNTFHMPQEV QEQVALVHSQ
FQRASTRTDP PDTQLSMDLA WALTDNPSDP ALLTRISHKL QLHTMADMKN ESIALHNMVI
STAGEPDGCV DQMSSLLKKL KDCVVTEDHA NDALTTRSAS IKHRSPIIPD EFRCPISLEL
MQDPVIVSSG QTYERSCIQK WLDSGHKTCP KTQQPLSHTS LTPNFVLKSL ISQWCEANGI
ELPKNKQNSR DKKAAKSSDY DHAGLVSLMN RLRSGNQDEQ RAAAGEIRLL AKRNVNNRIC
IAEAGAIPLL VNLLSSSDPR TQEHAVTALL NLSIHENNKA SIVDSHAIPK IVEVLKTGSM
ETRENAAATL FSLSVVDENK VTIGAAGAIP PLINLLCDGS PRGKKDAATA IFNLCIYQGN
KVRAVKAGIV IHLMNFLVDP TGGMIDEALS LLSILAGNPE GKIVIARSEP IPPLVEVIKT
GSPRNRENAA AILWLLCSAD TEQTLAAKAA GVEDALKELS ETGTDRAKRK ASSILELMHQ
ANEDSLKGNG H