PUB15_ARATH
ID PUB15_ARATH Reviewed; 660 AA.
AC Q681N2; Q9FII3;
DT 26-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT 26-FEB-2008, sequence version 2.
DT 25-MAY-2022, entry version 111.
DE RecName: Full=U-box domain-containing protein 15;
DE EC=2.3.2.27;
DE AltName: Full=Plant U-box protein 15;
DE AltName: Full=RING-type E3 ubiquitin transferase PUB15 {ECO:0000305};
GN Name=PUB15; OrderedLocusNames=At5g42340; ORFNames=MDH9.3;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10048488; DOI=10.1093/dnares/5.6.379;
RA Asamizu E., Sato S., Kaneko T., Nakamura Y., Kotani H., Miyajima N.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. VIII. Sequence
RT features of the regions of 1,081,958 bp covered by seventeen physically
RT assigned P1 and TAC clones.";
RL DNA Res. 5:379-391(1998).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP GENE FAMILY ORGANIZATION, AND NOMENCLATURE.
RX PubMed=11495788; DOI=10.1016/s1360-1385(01)01960-4;
RA Azevedo C., Santos-Rosa M.J., Shirasu K.;
RT "The U-box protein family in plants.";
RL Trends Plant Sci. 6:354-358(2001).
RN [5]
RP GENE FAMILY ORGANIZATION.
RX PubMed=14657406; DOI=10.1104/pp.103.029553;
RA Mudgil Y., Shiu S.-H., Stone S.L., Salt J.N., Goring D.R.;
RT "A large complement of the predicted Arabidopsis ARM repeat proteins are
RT members of the U-box E3 ubiquitin ligase family.";
RL Plant Physiol. 134:59-66(2004).
CC -!- FUNCTION: Functions as an E3 ubiquitin ligase. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine +
CC [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.;
CC EC=2.3.2.27;
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAB10475.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AB016888; BAB10475.1; ALT_INIT; Genomic_DNA.
DR EMBL; CP002688; AED94798.1; -; Genomic_DNA.
DR EMBL; AK175585; BAD43348.1; -; mRNA.
DR RefSeq; NP_199049.2; NM_123599.3.
DR AlphaFoldDB; Q681N2; -.
DR SMR; Q681N2; -.
DR STRING; 3702.AT5G42340.1; -.
DR PaxDb; Q681N2; -.
DR PRIDE; Q681N2; -.
DR ProteomicsDB; 226071; -.
DR EnsemblPlants; AT5G42340.1; AT5G42340.1; AT5G42340.
DR GeneID; 834240; -.
DR Gramene; AT5G42340.1; AT5G42340.1; AT5G42340.
DR KEGG; ath:AT5G42340; -.
DR Araport; AT5G42340; -.
DR TAIR; locus:2162276; AT5G42340.
DR eggNOG; KOG0167; Eukaryota.
DR HOGENOM; CLU_006348_5_1_1; -.
DR InParanoid; Q681N2; -.
DR OMA; SLAPNCA; -.
DR OrthoDB; 485562at2759; -.
DR PhylomeDB; Q681N2; -.
DR UniPathway; UPA00143; -.
DR PRO; PR:Q681N2; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q681N2; baseline and differential.
DR Genevisible; Q681N2; AT.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0004842; F:ubiquitin-protein transferase activity; IEA:InterPro.
DR GO; GO:0007166; P:cell surface receptor signaling pathway; IEA:InterPro.
DR CDD; cd16664; RING-Ubox_PUB; 1.
DR Gene3D; 1.20.930.20; -; 1.
DR Gene3D; 1.25.10.10; -; 1.
DR Gene3D; 3.30.40.10; -; 1.
DR InterPro; IPR036537; Adaptor_Cbl_N_dom_sf.
DR InterPro; IPR011989; ARM-like.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR000225; Armadillo.
DR InterPro; IPR045210; RING-Ubox_PUB.
DR InterPro; IPR003613; Ubox_domain.
DR InterPro; IPR013083; Znf_RING/FYVE/PHD.
DR Pfam; PF00514; Arm; 3.
DR Pfam; PF04564; U-box; 1.
DR SMART; SM00185; ARM; 5.
DR SMART; SM00504; Ubox; 1.
DR SUPFAM; SSF48371; SSF48371; 1.
DR PROSITE; PS50176; ARM_REPEAT; 3.
DR PROSITE; PS51698; U_BOX; 1.
PE 2: Evidence at transcript level;
KW Reference proteome; Repeat; Transferase; Ubl conjugation pathway.
FT CHAIN 1..660
FT /note="U-box domain-containing protein 15"
FT /id="PRO_0000322159"
FT DOMAIN 289..363
FT /note="U-box"
FT REPEAT 411..450
FT /note="ARM 1"
FT REPEAT 452..491
FT /note="ARM 2"
FT REPEAT 493..532
FT /note="ARM 3"
FT REPEAT 534..573
FT /note="ARM 4"
FT REPEAT 575..614
FT /note="ARM 5"
FT REGION 1..29
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..21
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 17
FT /note="N -> S (in Ref. 3; BAD43348)"
FT /evidence="ECO:0000305"
FT CONFLICT 301
FT /note="E -> G (in Ref. 3; BAD43348)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 660 AA; 73896 MW; 76BFA6A909E93D68 CRC64;
MVDVMDTDEE ATGDAENRDE EVTAEEPIHD EVVDAVEIHE EEVKEDDDDC EGLVSDIVSI
VEFLDQINGY RRTQQKECFN LVRRLKILIP FLDEIRGFES PSCKHFLNRL RKVFLAAKKL
LETCSNGSKI YMALDGETMM TRFHSIYEKL NRVLVKAPFD ELMISGDAKD EIDSLCKQLK
KAKRRTDTQD IELAVDMMVV FSKTDPRNAD SAIIERLAKK LELQTIDDLK TETIAIQSLI
QDKGGLNIET KQHIIELLNK FKKLQGLEAT DILYQPVINK AITKSTSLIL PHEFLCPITL
EIMLDPVIIA TGQTYEKESI QKWFDAGHKT CPKTRQELDH LSLAPNFALK NLIMQWCEKN
NFKIPEKEVS PDSQNEQKDE VSLLVEALSS SQLEEQRRSV KQMRLLAREN PENRVLIANA
GAIPLLVQLL SYPDSGIQEN AVTTLLNLSI DEVNKKLISN EGAIPNIIEI LENGNREARE
NSAAALFSLS MLDENKVTIG LSNGIPPLVD LLQHGTLRGK KDALTALFNL SLNSANKGRA
IDAGIVQPLL NLLKDKNLGM IDEALSILLL LASHPEGRQA IGQLSFIETL VEFIRQGTPK
NKECATSVLL ELGSNNSSFI LAALQFGVYE YLVEITTSGT NRAQRKANAL IQLISKSEQI
