PUB16_ARATH
ID PUB16_ARATH Reviewed; 674 AA.
AC Q9LZW3;
DT 26-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 137.
DE RecName: Full=U-box domain-containing protein 16;
DE EC=2.3.2.27;
DE AltName: Full=Plant U-box protein 16;
DE AltName: Full=RING-type E3 ubiquitin transferase PUB16 {ECO:0000305};
GN Name=PUB16; OrderedLocusNames=At5g01830; ORFNames=T20L15_100;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130714; DOI=10.1038/35048507;
RA Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E.,
RA Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M.,
RA Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.,
RA Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M.,
RA Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L.,
RA O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D.,
RA Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M.,
RA Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L.,
RA Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B.,
RA Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P.,
RA Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M.,
RA Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D.,
RA Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A.,
RA Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I.,
RA Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T.,
RA Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A.,
RA McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U.,
RA Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W.,
RA Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M.,
RA Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S.,
RA Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G.,
RA Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W.,
RA Bevan M., Fransz P.F.;
RT "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.";
RL Nature 408:823-826(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Cheuk R.F., Chen H., Kim C.J., Shinn P., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP GENE FAMILY ORGANIZATION, AND NOMENCLATURE.
RX PubMed=11495788; DOI=10.1016/s1360-1385(01)01960-4;
RA Azevedo C., Santos-Rosa M.J., Shirasu K.;
RT "The U-box protein family in plants.";
RL Trends Plant Sci. 6:354-358(2001).
RN [6]
RP GENE FAMILY ORGANIZATION.
RX PubMed=14657406; DOI=10.1104/pp.103.029553;
RA Mudgil Y., Shiu S.-H., Stone S.L., Salt J.N., Goring D.R.;
RT "A large complement of the predicted Arabidopsis ARM repeat proteins are
RT members of the U-box E3 ubiquitin ligase family.";
RL Plant Physiol. 134:59-66(2004).
CC -!- FUNCTION: Functions as an E3 ubiquitin ligase. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine +
CC [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.;
CC EC=2.3.2.27;
CC -!- PATHWAY: Protein modification; protein ubiquitination.
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DR EMBL; AL162351; CAB82753.1; -; Genomic_DNA.
DR EMBL; CP002688; AED90398.1; -; Genomic_DNA.
DR EMBL; BT015367; AAU05490.1; -; mRNA.
DR EMBL; BT015725; AAU45223.1; -; mRNA.
DR EMBL; AK229397; BAF01259.1; -; mRNA.
DR PIR; T48204; T48204.
DR RefSeq; NP_195803.1; NM_120261.4.
DR AlphaFoldDB; Q9LZW3; -.
DR SMR; Q9LZW3; -.
DR STRING; 3702.AT5G01830.1; -.
DR PaxDb; Q9LZW3; -.
DR PRIDE; Q9LZW3; -.
DR ProteomicsDB; 226248; -.
DR EnsemblPlants; AT5G01830.1; AT5G01830.1; AT5G01830.
DR GeneID; 831850; -.
DR Gramene; AT5G01830.1; AT5G01830.1; AT5G01830.
DR KEGG; ath:AT5G01830; -.
DR Araport; AT5G01830; -.
DR TAIR; locus:2181077; AT5G01830.
DR eggNOG; KOG0167; Eukaryota.
DR HOGENOM; CLU_006348_5_2_1; -.
DR InParanoid; Q9LZW3; -.
DR OMA; VHAYRKR; -.
DR OrthoDB; 647075at2759; -.
DR PhylomeDB; Q9LZW3; -.
DR UniPathway; UPA00143; -.
DR PRO; PR:Q9LZW3; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9LZW3; baseline and differential.
DR Genevisible; Q9LZW3; AT.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0004842; F:ubiquitin-protein transferase activity; IEA:InterPro.
DR CDD; cd16664; RING-Ubox_PUB; 1.
DR Gene3D; 1.25.10.10; -; 1.
DR Gene3D; 3.30.40.10; -; 1.
DR InterPro; IPR011989; ARM-like.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR000225; Armadillo.
DR InterPro; IPR045210; RING-Ubox_PUB.
DR InterPro; IPR003613; Ubox_domain.
DR InterPro; IPR013083; Znf_RING/FYVE/PHD.
DR Pfam; PF00514; Arm; 1.
DR Pfam; PF04564; U-box; 1.
DR SMART; SM00185; ARM; 2.
DR SMART; SM00504; Ubox; 1.
DR SUPFAM; SSF48371; SSF48371; 1.
DR PROSITE; PS50176; ARM_REPEAT; 1.
DR PROSITE; PS51698; U_BOX; 1.
PE 2: Evidence at transcript level;
KW Reference proteome; Repeat; Transferase; Ubl conjugation pathway.
FT CHAIN 1..674
FT /note="U-box domain-containing protein 16"
FT /id="PRO_0000322160"
FT DOMAIN 273..347
FT /note="U-box"
FT REPEAT 399..438
FT /note="ARM 1"
FT REPEAT 441..481
FT /note="ARM 2"
FT REPEAT 484..523
FT /note="ARM 3"
SQ SEQUENCE 674 AA; 73600 MW; FD91FAED63F415CB CRC64;
MAVTLDSPSP ARKRRPLVVG SFESPKLSSD TKLTRSLFLA SHEISSMQPL PFILRRNSLS
LIRKVKILAS VFDELLLPRS QLVVYSQSAH LCFEEMQIVM QRIKSLIDDC SRVSKLWLLL
QIDIVAFNFH ELVTDLSTVL DILPLHDFDL SDDAQDLISL LTKQCSDSVQ FVDARDVALR
RKVTDTIAGI KHQISPDHST LIKIFNDLGL SDSASLTDEI QRLEDEIQDQ IDDRSKSAAA
SLIGLVRYSK CVLYGPSTPA PDFRRHQSLS DANIPADFRC PITLELMRDP VVVATGQTYD
RESIDLWIQS GHNTCPKTGQ VLKHTSLVPN RALKNLIVLW CRDQKIPFEL YGDGGGEPAP
CKEAVEFTKM MVSFLIEKLS VADSNGVVFE LRALAKSDTV ARACIAEAGA IPKLVRYLAT
ECPSLQINAV TTILNLSILE QNKTRIMETD GALNGVIEVL RSGATWEAKA NAAATLFSLA
GVSAYRRRLG RKARVVSGLV DLAKQGPTSS KRDALVAILN LVAERENVGR FVEAGVMGAA
GDAFQELPEE AVAVVEAVVR RGGLMAVSAA FSLIRLLGEV MREGADTTRE SAAATLVTMC
RKGGSELVAE MAAIPGIERV IWEMIGAGTA RGGRKAASLM RYLRRWAAGD THNTAAETQS
IVVPTPSRIF SPVL
