PUB19_ARATH
ID PUB19_ARATH Reviewed; 686 AA.
AC O80742;
DT 26-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1998, sequence version 1.
DT 25-MAY-2022, entry version 147.
DE RecName: Full=U-box domain-containing protein 19;
DE EC=2.3.2.27;
DE AltName: Full=Plant U-box protein 19;
DE AltName: Full=RING-type E3 ubiquitin transferase PUB19 {ECO:0000305};
GN Name=PUB19; OrderedLocusNames=At1g60190; ORFNames=T13D8.8;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP GENE FAMILY ORGANIZATION, AND NOMENCLATURE.
RX PubMed=11495788; DOI=10.1016/s1360-1385(01)01960-4;
RA Azevedo C., Santos-Rosa M.J., Shirasu K.;
RT "The U-box protein family in plants.";
RL Trends Plant Sci. 6:354-358(2001).
RN [4]
RP GENE FAMILY ORGANIZATION.
RX PubMed=14657406; DOI=10.1104/pp.103.029553;
RA Mudgil Y., Shiu S.-H., Stone S.L., Salt J.N., Goring D.R.;
RT "A large complement of the predicted Arabidopsis ARM repeat proteins are
RT members of the U-box E3 ubiquitin ligase family.";
RL Plant Physiol. 134:59-66(2004).
CC -!- FUNCTION: Functions as an E3 ubiquitin ligase. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine +
CC [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.;
CC EC=2.3.2.27;
CC -!- PATHWAY: Protein modification; protein ubiquitination.
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DR EMBL; AC004473; AAC24052.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE33665.1; -; Genomic_DNA.
DR PIR; T02271; T02271.
DR RefSeq; NP_176225.1; NM_104709.3.
DR AlphaFoldDB; O80742; -.
DR SMR; O80742; -.
DR STRING; 3702.AT1G60190.1; -.
DR iPTMnet; O80742; -.
DR PaxDb; O80742; -.
DR PRIDE; O80742; -.
DR ProteomicsDB; 224851; -.
DR EnsemblPlants; AT1G60190.1; AT1G60190.1; AT1G60190.
DR GeneID; 842314; -.
DR Gramene; AT1G60190.1; AT1G60190.1; AT1G60190.
DR KEGG; ath:AT1G60190; -.
DR Araport; AT1G60190; -.
DR TAIR; locus:2195563; AT1G60190.
DR eggNOG; KOG0167; Eukaryota.
DR HOGENOM; CLU_006348_5_2_1; -.
DR InParanoid; O80742; -.
DR OMA; VRKWRDC; -.
DR OrthoDB; 720131at2759; -.
DR PhylomeDB; O80742; -.
DR UniPathway; UPA00143; -.
DR PRO; PR:O80742; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; O80742; baseline and differential.
DR Genevisible; O80742; AT.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0004842; F:ubiquitin-protein transferase activity; IEA:InterPro.
DR GO; GO:0010029; P:regulation of seed germination; IGI:TAIR.
DR CDD; cd16664; RING-Ubox_PUB; 1.
DR Gene3D; 1.25.10.10; -; 1.
DR Gene3D; 3.30.40.10; -; 1.
DR InterPro; IPR011989; ARM-like.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR000225; Armadillo.
DR InterPro; IPR045210; RING-Ubox_PUB.
DR InterPro; IPR003613; Ubox_domain.
DR InterPro; IPR013083; Znf_RING/FYVE/PHD.
DR Pfam; PF00514; Arm; 1.
DR Pfam; PF04564; U-box; 1.
DR SMART; SM00185; ARM; 3.
DR SMART; SM00504; Ubox; 1.
DR SUPFAM; SSF48371; SSF48371; 1.
DR PROSITE; PS50176; ARM_REPEAT; 2.
DR PROSITE; PS51698; U_BOX; 1.
PE 2: Evidence at transcript level;
KW Reference proteome; Repeat; Transferase; Ubl conjugation pathway.
FT CHAIN 1..686
FT /note="U-box domain-containing protein 19"
FT /id="PRO_0000322163"
FT DOMAIN 277..351
FT /note="U-box"
FT REPEAT 406..445
FT /note="ARM 1"
FT REPEAT 448..489
FT /note="ARM 2"
FT REPEAT 491..533
FT /note="ARM 3"
FT REPEAT 536..577
FT /note="ARM 4"
FT REPEAT 579..620
FT /note="ARM 5"
SQ SEQUENCE 686 AA; 76014 MW; 59ED9D54AA062512 CRC64;
MIHTPTGSSR RILTFPAVNP CESISLTTLV DSLLQLAGEI LSFKPKHFST NKRSVKETLR
HVQTLVIFFE ELRIQIRVGS IPAGRSVILS LSELHVIFQK LKFLLDDCTR DGAKLYMLMN
SGQVSAHFRD LTRSISTSLD TFPVRSVDLP GEVNELIYLV MRQTRKSEAR PDRDDKRAID
SVYWFFNLFE NRINPNSDEI LRVLDHIGVR KWRDCVKEID FLREEISVGK KSNIEIELLS
NLMGFICYCR CVILRGIDVD DEEKDKEEDD LMMVRSLNVD DLRCPISLEI MSDPVVLESG
HTYDRSSITK WFASGNITCP KTGKTLVSTV LVDNFSVKQV IQSYSKQNGV VMGQKGKKKV
DVAESLAAEE AGKLTAEFLA GELIKGDEEE MVKALVEIRI LTKTSTFYRS CLVEAGVVES
LMKILRSDDP RIQENAMAGI MNLSKDIAGK TRIVGEDGGG LRLIVEVLND GARRESRQYA
AAALFYLSSL GDYSRLIGEI SDAIPGLVRI VKSCDYGDSA KRNALIAIRS LLMNQPDNHW
RILAAGIVPV LLDLVKSEEI SDGVTADSMA ILAKMAEYPD GMISVLRRGG LKLAVKILGS
SEVSPATKQH CVALLLNLCH NGGSDVVGSL AKNPSIMGSL YTASSNGELG GGKKASALIK
MIHEFQERKT GPGEPVLERE RFVHAW