PUB20_ARATH
ID PUB20_ARATH Reviewed; 431 AA.
AC Q9C8D1; Q8LB50;
DT 26-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 125.
DE RecName: Full=U-box domain-containing protein 20;
DE EC=2.3.2.27;
DE AltName: Full=Plant U-box protein 20;
DE AltName: Full=RING-type E3 ubiquitin transferase PUB20 {ECO:0000305};
GN Name=PUB20; OrderedLocusNames=At1g66160; ORFNames=F15E12.6;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP GENE FAMILY ORGANIZATION, AND NOMENCLATURE.
RX PubMed=11495788; DOI=10.1016/s1360-1385(01)01960-4;
RA Azevedo C., Santos-Rosa M.J., Shirasu K.;
RT "The U-box protein family in plants.";
RL Trends Plant Sci. 6:354-358(2001).
RN [6]
RP GENE FAMILY ORGANIZATION.
RX PubMed=14657406; DOI=10.1104/pp.103.029553;
RA Mudgil Y., Shiu S.-H., Stone S.L., Salt J.N., Goring D.R.;
RT "A large complement of the predicted Arabidopsis ARM repeat proteins are
RT members of the U-box E3 ubiquitin ligase family.";
RL Plant Physiol. 134:59-66(2004).
CC -!- FUNCTION: Functions as an E3 ubiquitin ligase. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine +
CC [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.;
CC EC=2.3.2.27;
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC -!- INTERACTION:
CC Q9C8D1; Q9LEZ3: BIM1; NbExp=3; IntAct=EBI-25516153, EBI-617095;
CC Q9C8D1; Q17TI5: BRX; NbExp=3; IntAct=EBI-25516153, EBI-4426649;
CC Q9C8D1; O23160: MYB73; NbExp=3; IntAct=EBI-25516153, EBI-25506855;
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=1;
CC Comment=A number of isoforms are produced. According to EST
CC sequences.;
CC Name=1;
CC IsoId=Q9C8D1-1; Sequence=Displayed;
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DR EMBL; AC026480; AAG51307.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE34470.1; -; Genomic_DNA.
DR EMBL; AY034913; AAK59420.1; -; mRNA.
DR EMBL; AY063104; AAL34278.1; -; mRNA.
DR EMBL; AY087421; AAM64969.1; -; mRNA.
DR PIR; D96686; D96686.
DR RefSeq; NP_564866.1; NM_105287.2. [Q9C8D1-1]
DR AlphaFoldDB; Q9C8D1; -.
DR SMR; Q9C8D1; -.
DR BioGRID; 28152; 5.
DR IntAct; Q9C8D1; 3.
DR STRING; 3702.AT1G66160.1; -.
DR PaxDb; Q9C8D1; -.
DR PRIDE; Q9C8D1; -.
DR ProteomicsDB; 226451; -. [Q9C8D1-1]
DR EnsemblPlants; AT1G66160.1; AT1G66160.1; AT1G66160. [Q9C8D1-1]
DR GeneID; 842931; -.
DR Gramene; AT1G66160.1; AT1G66160.1; AT1G66160. [Q9C8D1-1]
DR KEGG; ath:AT1G66160; -.
DR Araport; AT1G66160; -.
DR TAIR; locus:2013850; AT1G66160.
DR eggNOG; ENOG502QS2D; Eukaryota.
DR HOGENOM; CLU_006348_1_1_1; -.
DR InParanoid; Q9C8D1; -.
DR OrthoDB; 556624at2759; -.
DR PhylomeDB; Q9C8D1; -.
DR UniPathway; UPA00143; -.
DR PRO; PR:Q9C8D1; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9C8D1; baseline and differential.
DR Genevisible; Q9C8D1; AT.
DR GO; GO:0061630; F:ubiquitin protein ligase activity; IEA:InterPro.
DR CDD; cd16664; RING-Ubox_PUB; 1.
DR Gene3D; 1.25.10.10; -; 1.
DR Gene3D; 3.30.40.10; -; 1.
DR InterPro; IPR011989; ARM-like.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR045185; PUB22/23/24-like.
DR InterPro; IPR045210; RING-Ubox_PUB.
DR InterPro; IPR003613; Ubox_domain.
DR InterPro; IPR013083; Znf_RING/FYVE/PHD.
DR PANTHER; PTHR22849; PTHR22849; 1.
DR Pfam; PF04564; U-box; 1.
DR SMART; SM00504; Ubox; 1.
DR SUPFAM; SSF48371; SSF48371; 1.
DR PROSITE; PS51698; U_BOX; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Reference proteome; Transferase;
KW Ubl conjugation pathway.
FT CHAIN 1..431
FT /note="U-box domain-containing protein 20"
FT /id="PRO_0000322164"
FT DOMAIN 32..106
FT /note="U-box"
FT CONFLICT 123
FT /note="V -> I (in Ref. 4; AAM64969)"
FT /evidence="ECO:0000305"
FT CONFLICT 150
FT /note="T -> K (in Ref. 4; AAM64969)"
FT /evidence="ECO:0000305"
FT CONFLICT 199
FT /note="M -> T (in Ref. 4; AAM64969)"
FT /evidence="ECO:0000305"
FT CONFLICT 204
FT /note="L -> S (in Ref. 4; AAM64969)"
FT /evidence="ECO:0000305"
FT CONFLICT 224
FT /note="R -> K (in Ref. 4; AAM64969)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 431 AA; 48212 MW; 55AF915F37B85C0E CRC64;
MGLSLRVRRR GGSVSKKEII PVTSCSEEVE ITIPSQFQCP ISYELMKDPV IIASGITYDR
ENIEKWFESG YQTCPVTNTV LTSLEQIPNH TIRRMIQGWC GSSLGGGIER IPTPRVPVTS
HQVSEICERL SAATRRGDYA ACMEMVTKMT RLGKESERNR KCVKENGAGL VLCVCFDAFS
ENANASLLLE ETVSVLTWML PIGLEGQSKL TTTSSFNRLV ELLRNGDQNA AFLIKELLEL
NVTHVHALTK INGVQEAFMK SINRDSTCVN SLISIHHMIL TNQETVSRFL ELDLVNITVE
MLVDSENSVC EKALTVLNVI CETKEGREKV RRNKLVIPIL VKKILKISEK KDLVSVMWKV
CKSGDGSEVE EALRLGAFKK LVVMLQVGCG EGTKEKVTEL LKMMNKVMKM NGFVDRSYSS
SIEFKHVKKP F
