ID   PUB21_ARATH             Reviewed;         435 AA.
AC   Q5PNY6; Q9FGI7;
DT   26-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT   04-JAN-2005, sequence version 1.
DT   03-AUG-2022, entry version 121.
DE   RecName: Full=U-box domain-containing protein 21;
DE            EC=2.3.2.27;
DE   AltName: Full=Plant U-box protein 21;
DE   AltName: Full=RING-type E3 ubiquitin transferase PUB21 {ECO:0000305};
GN   Name=PUB21; OrderedLocusNames=At5g37490; ORFNames=MPA22.3;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RA   Kaneko T., Katoh T., Asamizu E., Sato S., Nakamura Y., Kotani H.,
RA   Tabata S.;
RT   "Structural analysis of Arabidopsis thaliana chromosome 5. XI.";
RL   Submitted (APR-1999) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RA   Cheuk R.F., Chen H., Kim C.J., Shinn P., Ecker J.R.;
RT   "Arabidopsis ORF clones.";
RL   Submitted (DEC-2004) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   GENE FAMILY ORGANIZATION, AND NOMENCLATURE.
RX   PubMed=11495788; DOI=10.1016/s1360-1385(01)01960-4;
RA   Azevedo C., Santos-Rosa M.J., Shirasu K.;
RT   "The U-box protein family in plants.";
RL   Trends Plant Sci. 6:354-358(2001).
RN   [5]
RP   GENE FAMILY ORGANIZATION.
RX   PubMed=14657406; DOI=10.1104/pp.103.029553;
RA   Mudgil Y., Shiu S.-H., Stone S.L., Salt J.N., Goring D.R.;
RT   "A large complement of the predicted Arabidopsis ARM repeat proteins are
RT   members of the U-box E3 ubiquitin ligase family.";
RL   Plant Physiol. 134:59-66(2004).
CC   -!- FUNCTION: Functions as an E3 ubiquitin ligase. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine +
CC         [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC         cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.;
CC         EC=2.3.2.27;
CC   -!- PATHWAY: Protein modification; protein ubiquitination.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=BAB10667.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AB025630; BAB10667.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; CP002688; AED94197.1; -; Genomic_DNA.
DR   EMBL; BT020311; AAV85666.1; -; mRNA.
DR   EMBL; BT020439; AAW30018.1; -; mRNA.
DR   RefSeq; NP_198565.1; NM_123108.4.
DR   AlphaFoldDB; Q5PNY6; -.
DR   SMR; Q5PNY6; -.
DR   STRING; 3702.AT5G37490.1; -.
DR   PaxDb; Q5PNY6; -.
DR   PRIDE; Q5PNY6; -.
DR   EnsemblPlants; AT5G37490.1; AT5G37490.1; AT5G37490.
DR   GeneID; 833727; -.
DR   Gramene; AT5G37490.1; AT5G37490.1; AT5G37490.
DR   KEGG; ath:AT5G37490; -.
DR   Araport; AT5G37490; -.
DR   TAIR; locus:2169861; AT5G37490.
DR   eggNOG; ENOG502QS2D; Eukaryota.
DR   HOGENOM; CLU_006348_1_1_1; -.
DR   InParanoid; Q5PNY6; -.
DR   OMA; RVDCFDG; -.
DR   OrthoDB; 556624at2759; -.
DR   PhylomeDB; Q5PNY6; -.
DR   UniPathway; UPA00143; -.
DR   PRO; PR:Q5PNY6; -.
DR   Proteomes; UP000006548; Chromosome 5.
DR   ExpressionAtlas; Q5PNY6; baseline and differential.
DR   Genevisible; Q5PNY6; AT.
DR   GO; GO:0061630; F:ubiquitin protein ligase activity; IEA:InterPro.
DR   CDD; cd16664; RING-Ubox_PUB; 1.
DR   Gene3D; 1.25.10.10; -; 1.
DR   Gene3D; 3.30.40.10; -; 1.
DR   InterPro; IPR011989; ARM-like.
DR   InterPro; IPR016024; ARM-type_fold.
DR   InterPro; IPR045185; PUB22/23/24-like.
DR   InterPro; IPR045210; RING-Ubox_PUB.
DR   InterPro; IPR003613; Ubox_domain.
DR   InterPro; IPR013083; Znf_RING/FYVE/PHD.
DR   PANTHER; PTHR22849; PTHR22849; 1.
DR   Pfam; PF04564; U-box; 1.
DR   SMART; SM00504; Ubox; 1.
DR   SUPFAM; SSF48371; SSF48371; 1.
DR   PROSITE; PS51698; U_BOX; 1.
PE   2: Evidence at transcript level;
KW   Reference proteome; Repeat; Transferase; Ubl conjugation pathway.
FT   CHAIN           1..435
FT                   /note="U-box domain-containing protein 21"
FT                   /id="PRO_0000322165"
FT   DOMAIN          30..104
FT                   /note="U-box"
FT   REPEAT          202..241
FT                   /note="ARM 1"
FT   REPEAT          245..285
FT                   /note="ARM 2"
FT   REPEAT          288..327
FT                   /note="ARM 3"
FT   REPEAT          329..369
FT                   /note="ARM 4"
SQ   SEQUENCE   435 AA;  48725 MW;  240ACC84350C308D CRC64;
     MGFLWRTRSN EKKITPVLSW PESEPESEIT IPPEFQCPIS IDLMKDPVII STGITYDRVS
     IETWINSGNK TCPVTNTVLT TFDQIPNHTI RKMIQGWCVE KGSPLIQRIP TPRVPLMPCE
     VYEISRKLSS ATRRGDYEKC GVIIEKIKKL GDESEKNRKC VNENSVGWVL CDCFDKFSGD
     EKLTFMLNEI LSLLTWMFPI GLEGISKLAS ATSFRCVAGL LKSTDDSVRQ NAAFIMKEIL
     SLDETRVHSF AVENGVAEAL VKLIRDSVSS SSTKSSLIAI YQMVLQKPEI ASEFLEIGLV
     SITVEMIVDA ENSVCEKALA VLDAICETEH GREEVRKNAL VMPLLVKKIA KVSELATRSS
     MSMILKLWKT GNTVAVEDAV RLGAFQKVLL VLQVGYGEET KEKATELLKM MNTQMKLMSD
     CVDSLKEFKY IKKPF