PUB24_ORYSJ
ID PUB24_ORYSJ Reviewed; 824 AA.
AC Q10FT0; Q7Y097;
DT 16-OCT-2019, integrated into UniProtKB/Swiss-Prot.
DT 22-AUG-2006, sequence version 1.
DT 03-AUG-2022, entry version 125.
DE RecName: Full=U-box domain-containing protein 24 {ECO:0000305};
DE EC=2.3.2.27 {ECO:0000269|PubMed:30920691};
DE AltName: Full=Plant U-box protein 24 {ECO:0000303|PubMed:30920691};
DE Short=OsPUB24 {ECO:0000303|PubMed:30920691};
DE AltName: Full=RING-type E3 ubiquitin transferase PUB24 {ECO:0000305};
GN Name=PUB24 {ECO:0000303|PubMed:30920691};
GN OrderedLocusNames=Os03g0657100 {ECO:0000312|EMBL:BAF12727.1},
GN LOC_Os03g45420 {ECO:0000312|EMBL:ABF97977.1};
GN ORFNames=OSJNBa0075A22.19 {ECO:0000312|EMBL:AAP50990.1};
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16109971; DOI=10.1101/gr.3869505;
RG The rice chromosome 3 sequencing consortium;
RA Buell C.R., Yuan Q., Ouyang S., Liu J., Zhu W., Wang A., Maiti R., Haas B.,
RA Wortman J., Pertea M., Jones K.M., Kim M., Overton L., Tsitrin T.,
RA Fadrosh D., Bera J., Weaver B., Jin S., Johri S., Reardon M., Webb K.,
RA Hill J., Moffat K., Tallon L., Van Aken S., Lewis M., Utterback T.,
RA Feldblyum T., Zismann V., Iobst S., Hsiao J., de Vazeille A.R.,
RA Salzberg S.L., White O., Fraser C.M., Yu Y., Kim H., Rambo T., Currie J.,
RA Collura K., Kernodle-Thompson S., Wei F., Kudrna K., Ammiraju J.S.S.,
RA Luo M., Goicoechea J.L., Wing R.A., Henry D., Oates R., Palmer M.,
RA Pries G., Saski C., Simmons J., Soderlund C., Nelson W., de la Bastide M.,
RA Spiegel L., Nascimento L., Huang E., Preston R., Zutavern T., Palmer L.,
RA O'Shaughnessy A., Dike S., McCombie W.R., Minx P., Cordum H., Wilson R.,
RA Jin W., Lee H.R., Jiang J., Jackson S.;
RT "Sequence, annotation, and analysis of synteny between rice chromosome 3
RT and diverged grass species.";
RL Genome Res. 15:1284-1291(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [5]
RP FUNCTION, CATALYTIC ACTIVITY, INTERACTION WITH BZR1; BZR2; BZR3 AND GSK2,
RP SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE, AUTOUBIQUITINATION,
RP PHOSPHORYLATION, AND MUTAGENESIS OF CYS-20.
RX PubMed=30920691; DOI=10.1111/tpj.14332;
RA Min H.J., Cui L.H., Oh T.R., Kim J.H., Kim T.W., Kim W.T.;
RT "OsBZR1 turnover mediated by OsSK22-regulated U-box E3 ligase OsPUB24 in
RT rice BR response.";
RL Plant J. 99:426-438(2019).
CC -!- FUNCTION: E3 ubiquitin-protein ligase that functions as a negative
CC regulator of brassinosteroid (BR) signaling (PubMed:30920691). Targets
CC BZR1, a positive regulator of BR signaling pathway, and promotes its
CC degradation via the ubiquitin-26S proteasome pathway (PubMed:30920691).
CC {ECO:0000269|PubMed:30920691}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine +
CC [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.;
CC EC=2.3.2.27; Evidence={ECO:0000269|PubMed:30920691};
CC -!- PATHWAY: Protein modification; protein ubiquitination. {ECO:0000305}.
CC -!- SUBUNIT: Interacts with BZR1, BZR2, BZR3 and GSK2.
CC {ECO:0000269|PubMed:30920691}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytosol {ECO:0000269|PubMed:30920691}.
CC Nucleus {ECO:0000269|PubMed:30920691}.
CC -!- PTM: Auto-ubiquitinated. {ECO:0000269|PubMed:30920691}.
CC -!- PTM: Phosproylated by GSK2 (PubMed:30920691). Phosphorylation of PUB24
CC increases its cellular stability (PubMed:30920691).
CC {ECO:0000269|PubMed:30920691}.
CC -!- DISRUPTION PHENOTYPE: Pleiotropic phenotypes, such as reduced height,
CC enlarged lamina joint angles, increased tiller number, decreased
CC panicle length and reduced primary branches per panicle, due to
CC hypersensitivity to brassinosteroid. {ECO:0000269|PubMed:30920691}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAP50990.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC133859; AAP50990.1; ALT_SEQ; Genomic_DNA.
DR EMBL; DP000009; ABF97977.1; -; Genomic_DNA.
DR EMBL; AP008209; BAF12727.1; -; Genomic_DNA.
DR EMBL; AP014959; BAS85568.1; -; Genomic_DNA.
DR RefSeq; XP_015631680.1; XM_015776194.1.
DR AlphaFoldDB; Q10FT0; -.
DR SMR; Q10FT0; -.
DR STRING; 4530.OS03T0657100-01; -.
DR PaxDb; Q10FT0; -.
DR PRIDE; Q10FT0; -.
DR EnsemblPlants; Os03t0657100-01; Os03t0657100-01; Os03g0657100.
DR GeneID; 4333620; -.
DR Gramene; Os03t0657100-01; Os03t0657100-01; Os03g0657100.
DR KEGG; osa:4333620; -.
DR eggNOG; KOG0167; Eukaryota.
DR HOGENOM; CLU_004912_0_0_1; -.
DR InParanoid; Q10FT0; -.
DR OMA; ECRENGQ; -.
DR OrthoDB; 268677at2759; -.
DR UniPathway; UPA00143; -.
DR Proteomes; UP000000763; Chromosome 3.
DR Proteomes; UP000059680; Chromosome 3.
DR ExpressionAtlas; Q10FT0; baseline and differential.
DR GO; GO:0005829; C:cytosol; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0004842; F:ubiquitin-protein transferase activity; IEA:InterPro.
DR GO; GO:0009742; P:brassinosteroid mediated signaling pathway; IEA:UniProtKB-KW.
DR CDD; cd16664; RING-Ubox_PUB; 1.
DR Gene3D; 1.25.10.10; -; 3.
DR Gene3D; 3.30.40.10; -; 1.
DR InterPro; IPR011989; ARM-like.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR000225; Armadillo.
DR InterPro; IPR045210; RING-Ubox_PUB.
DR InterPro; IPR003613; Ubox_domain.
DR InterPro; IPR013083; Znf_RING/FYVE/PHD.
DR Pfam; PF04564; U-box; 1.
DR SMART; SM00185; ARM; 9.
DR SMART; SM00504; Ubox; 1.
DR SUPFAM; SSF48371; SSF48371; 2.
DR PROSITE; PS51698; U_BOX; 1.
PE 1: Evidence at protein level;
KW Brassinosteroid signaling pathway; Cytoplasm; Nucleus; Phosphoprotein;
KW Reference proteome; Repeat; Transferase; Ubl conjugation;
KW Ubl conjugation pathway.
FT CHAIN 1..824
FT /note="U-box domain-containing protein 24"
FT /id="PRO_0000448266"
FT DOMAIN 13..92
FT /note="U-box"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01034"
FT REPEAT 133..172
FT /note="ARM 1"
FT /evidence="ECO:0000255"
FT REPEAT 175..214
FT /note="ARM 2"
FT /evidence="ECO:0000255"
FT REPEAT 217..258
FT /note="ARM 3"
FT /evidence="ECO:0000255"
FT REPEAT 260..299
FT /note="ARM 4"
FT /evidence="ECO:0000255"
FT REPEAT 300..339
FT /note="ARM 5"
FT /evidence="ECO:0000255"
FT REPEAT 341..385
FT /note="ARM 6"
FT /evidence="ECO:0000255"
FT REPEAT 396..435
FT /note="ARM 7"
FT /evidence="ECO:0000255"
FT REPEAT 441..481
FT /note="ARM 8"
FT /evidence="ECO:0000255"
FT REPEAT 486..525
FT /note="ARM 9"
FT /evidence="ECO:0000255"
FT MUTAGEN 20
FT /note="C->Y: Loss of E3 ubiquitin-protein ligase activity."
FT /evidence="ECO:0000269|PubMed:30920691"
SQ SEQUENCE 824 AA; 88483 MW; B08D77AF82B1D039 CRC64;
MAGEGVEMSE EEGAFEAFVC PLTKQVMRDP VTIETGQTFE REAILKWFRE CRDNGRRPTC
PLTQRELRDT EVSPSVALRS VIHEWRARNE EKDLDRACAS LVGGFAGHAG DEEEEESALR
ALVHVSQICQ RSAASKDLVR RRGVLRAVAE MLKSGSRRLR LKSLQVLRVL VEDNDDNKEE
LGKGDTIRTI IKFLSNEHVQ ERELAVSLLH ELSGHEPTCE RIGAVYGAIL LLVGMGSSKS
ESAVAVDKAE STLRNLDRFD ANVKQMADNG RLQPLLTRLL RGEPDTRVAM ADYLGELALA
NDDKAAVAEQ AGPLLVGMLR TGATPAKEAT LKALREISSS EASAKLLLQR AGVLPPLVND
VLFSTGHLPM KLKELAATIL ANLVASGADF RSIPLDDDED DDGGGGGRGR RRTLLSEDVV
HSQLHLISNT GPAIGCRLLS VLAGLTSSRA TVADVVAAVK SSGATISLIQ FIEAAHRDIR
VESLKLLRNL APYMGAELAD ALGGSLSSLL RAISSDGGGV TEEQAAAVGL LGDLPEGDSS
LTRQLFDLGA FRALAPKLAE LRRGTIRGGN RYVTPLTEGV VKVMYRVTCA LEEDAEYVEF
AREAGLAPLF VELLHTNGMD TVQLYSAMAL EKLSLQSSHL TAIPAPPSPP AGFGCACLGR
RPAAAAVPAG VCRVHGGFCS LRETFCLAQA DGGKAVERLV ACLDHLDGRV VEAALAALST
LVCDGVDARE GVVVLGEADG LRPVVDIMVE SRTEALQRRA VWAVERILRV EEIAGEVAAD
QTVASALVEA YRNGDPRTRQ TAERALRHLD RIPNFSAAFQ SKRS
