PUB25_ARATH
ID PUB25_ARATH Reviewed; 421 AA.
AC Q9LT79; Q0WMA7;
DT 26-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 130.
DE RecName: Full=U-box domain-containing protein 25;
DE EC=2.3.2.27;
DE AltName: Full=Plant U-box protein 25;
DE AltName: Full=RING-type E3 ubiquitin transferase PUB25 {ECO:0000305};
GN Name=PUB25; OrderedLocusNames=At3g19380; ORFNames=MLD14.11;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10819329; DOI=10.1093/dnares/7.2.131;
RA Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence
RT features of the regions of 4,504,864 bp covered by sixty P1 and TAC
RT clones.";
RL DNA Res. 7:131-135(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Shinn P., Chen H., Kim C.J., Quinitio C., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (JUN-2006) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 3-421.
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP GENE FAMILY ORGANIZATION, AND NOMENCLATURE.
RX PubMed=11495788; DOI=10.1016/s1360-1385(01)01960-4;
RA Azevedo C., Santos-Rosa M.J., Shirasu K.;
RT "The U-box protein family in plants.";
RL Trends Plant Sci. 6:354-358(2001).
RN [7]
RP GENE FAMILY ORGANIZATION.
RX PubMed=14657406; DOI=10.1104/pp.103.029553;
RA Mudgil Y., Shiu S.-H., Stone S.L., Salt J.N., Goring D.R.;
RT "A large complement of the predicted Arabidopsis ARM repeat proteins are
RT members of the U-box E3 ubiquitin ligase family.";
RL Plant Physiol. 134:59-66(2004).
CC -!- FUNCTION: Functions as an E3 ubiquitin ligase. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine +
CC [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.;
CC EC=2.3.2.27;
CC -!- PATHWAY: Protein modification; protein ubiquitination.
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DR EMBL; AB025624; BAB02462.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE76233.1; -; Genomic_DNA.
DR EMBL; BT025982; ABG25071.1; -; mRNA.
DR EMBL; AY086503; AAM63504.1; -; mRNA.
DR EMBL; AK229923; BAF01749.1; -; mRNA.
DR RefSeq; NP_566632.1; NM_112825.4.
DR AlphaFoldDB; Q9LT79; -.
DR SMR; Q9LT79; -.
DR STRING; 3702.AT3G19380.1; -.
DR PaxDb; Q9LT79; -.
DR PRIDE; Q9LT79; -.
DR ProteomicsDB; 226077; -.
DR EnsemblPlants; AT3G19380.1; AT3G19380.1; AT3G19380.
DR GeneID; 821472; -.
DR Gramene; AT3G19380.1; AT3G19380.1; AT3G19380.
DR KEGG; ath:AT3G19380; -.
DR Araport; AT3G19380; -.
DR TAIR; locus:2090604; AT3G19380.
DR eggNOG; ENOG502QRXY; Eukaryota.
DR HOGENOM; CLU_006348_1_1_1; -.
DR InParanoid; Q9LT79; -.
DR OMA; SKNTRHV; -.
DR OrthoDB; 1275246at2759; -.
DR PhylomeDB; Q9LT79; -.
DR UniPathway; UPA00143; -.
DR PRO; PR:Q9LT79; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q9LT79; baseline and differential.
DR Genevisible; Q9LT79; AT.
DR GO; GO:0061630; F:ubiquitin protein ligase activity; IDA:TAIR.
DR GO; GO:0098542; P:defense response to other organism; IMP:TAIR.
DR GO; GO:0016567; P:protein ubiquitination; IDA:TAIR.
DR CDD; cd16664; RING-Ubox_PUB; 1.
DR Gene3D; 1.25.10.10; -; 1.
DR Gene3D; 3.30.40.10; -; 1.
DR InterPro; IPR011989; ARM-like.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR045185; PUB22/23/24-like.
DR InterPro; IPR045210; RING-Ubox_PUB.
DR InterPro; IPR003613; Ubox_domain.
DR InterPro; IPR013083; Znf_RING/FYVE/PHD.
DR PANTHER; PTHR22849; PTHR22849; 1.
DR Pfam; PF04564; U-box; 1.
DR SMART; SM00504; Ubox; 1.
DR SUPFAM; SSF48371; SSF48371; 1.
DR PROSITE; PS51698; U_BOX; 1.
PE 2: Evidence at transcript level;
KW Reference proteome; Transferase; Ubl conjugation pathway.
FT CHAIN 1..421
FT /note="U-box domain-containing protein 25"
FT /id="PRO_0000322169"
FT DOMAIN 13..88
FT /note="U-box"
FT CONFLICT 148
FT /note="A -> T (in Ref. 5; BAF01749)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 421 AA; 46056 MW; 52CADD00E814D2F2 CRC64;
MPRNIEPLDL GIQIPYHFRC PISLELMQDP VTVCTGQTYD RASIESWVSI GNNTTCPVTR
APLSDFTLIP NHTLRRLIQE WCVANRSNGV ERIPTPKQPA DPTSVRALLS QASAITGTHV
SVRSRAAALR RLRGFARDSD KNRVLIAAHN ATEILIKILF SETTSSELVS ESLALLVMLP
ITEPNQFVSI SSDPGRVEFL TRLLFDSSIE TRVNAAALIE IVSTGTKSAD LKGSISNSES
VFEGVLDLLR NPISSRRALK IGIKTLFALC SVKSTRHIAI TAGAPEILID RLAADFDRCD
TERALATVEL LCRTPEGCAA FGEHALTVPL LVKTILRVSD RATEYAAGAL LALCTAEERW
REEAAGAGVV VQLLLMVQSE CTERAKKKAQ KLLKLLRDSW PDYNSFANSD DFGCSSQVVP
F
