ID   PUB27_ARATH             Reviewed;         420 AA.
AC   Q9FLF4;
DT   26-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   03-AUG-2022, entry version 121.
DE   RecName: Full=U-box domain-containing protein 27;
DE            EC=2.3.2.27;
DE   AltName: Full=Plant U-box protein 27;
DE   AltName: Full=RING-type E3 ubiquitin transferase PUB27 {ECO:0000305};
GN   Name=PUB27; OrderedLocusNames=At5g64660; ORFNames=MUB3.18;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=9628582; DOI=10.1093/dnares/5.1.41;
RA   Sato S., Kaneko T., Kotani H., Nakamura Y., Asamizu E., Miyajima N.,
RA   Tabata S.;
RT   "Structural analysis of Arabidopsis thaliana chromosome 5. IV. Sequence
RT   features of the regions of 1,456,315 bp covered by nineteen physically
RT   assigned P1 and TAC clones.";
RL   DNA Res. 5:41-54(1998).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RA   Shinn P., Chen H., Kim C.J., Quinitio C., Ecker J.R.;
RT   "Arabidopsis ORF clones.";
RL   Submitted (MAY-2006) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   GENE FAMILY ORGANIZATION, AND NOMENCLATURE.
RX   PubMed=11495788; DOI=10.1016/s1360-1385(01)01960-4;
RA   Azevedo C., Santos-Rosa M.J., Shirasu K.;
RT   "The U-box protein family in plants.";
RL   Trends Plant Sci. 6:354-358(2001).
RN   [5]
RP   GENE FAMILY ORGANIZATION.
RX   PubMed=14657406; DOI=10.1104/pp.103.029553;
RA   Mudgil Y., Shiu S.-H., Stone S.L., Salt J.N., Goring D.R.;
RT   "A large complement of the predicted Arabidopsis ARM repeat proteins are
RT   members of the U-box E3 ubiquitin ligase family.";
RL   Plant Physiol. 134:59-66(2004).
CC   -!- FUNCTION: Functions as an E3 ubiquitin ligase. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine +
CC         [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC         cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.;
CC         EC=2.3.2.27;
CC   -!- PATHWAY: Protein modification; protein ubiquitination.
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DR   EMBL; AB010076; BAB11433.1; -; Genomic_DNA.
DR   EMBL; CP002688; AED97933.1; -; Genomic_DNA.
DR   EMBL; BT023419; AAY56410.1; -; mRNA.
DR   EMBL; BT025629; ABF59047.1; -; mRNA.
DR   RefSeq; NP_201271.1; NM_125862.5.
DR   AlphaFoldDB; Q9FLF4; -.
DR   SMR; Q9FLF4; -.
DR   STRING; 3702.AT5G64660.1; -.
DR   iPTMnet; Q9FLF4; -.
DR   PaxDb; Q9FLF4; -.
DR   PRIDE; Q9FLF4; -.
DR   EnsemblPlants; AT5G64660.1; AT5G64660.1; AT5G64660.
DR   GeneID; 836587; -.
DR   Gramene; AT5G64660.1; AT5G64660.1; AT5G64660.
DR   KEGG; ath:AT5G64660; -.
DR   Araport; AT5G64660; -.
DR   TAIR; locus:2174814; AT5G64660.
DR   eggNOG; ENOG502QTKN; Eukaryota.
DR   HOGENOM; CLU_006348_1_1_1; -.
DR   InParanoid; Q9FLF4; -.
DR   OMA; HRLIQIW; -.
DR   OrthoDB; 989243at2759; -.
DR   PhylomeDB; Q9FLF4; -.
DR   UniPathway; UPA00143; -.
DR   PRO; PR:Q9FLF4; -.
DR   Proteomes; UP000006548; Chromosome 5.
DR   ExpressionAtlas; Q9FLF4; baseline and differential.
DR   Genevisible; Q9FLF4; AT.
DR   GO; GO:0061630; F:ubiquitin protein ligase activity; IEA:InterPro.
DR   CDD; cd16664; RING-Ubox_PUB; 1.
DR   Gene3D; 1.25.10.10; -; 1.
DR   Gene3D; 3.30.40.10; -; 1.
DR   InterPro; IPR011989; ARM-like.
DR   InterPro; IPR016024; ARM-type_fold.
DR   InterPro; IPR045185; PUB22/23/24-like.
DR   InterPro; IPR045210; RING-Ubox_PUB.
DR   InterPro; IPR003613; Ubox_domain.
DR   InterPro; IPR013083; Znf_RING/FYVE/PHD.
DR   PANTHER; PTHR22849; PTHR22849; 1.
DR   Pfam; PF04564; U-box; 1.
DR   SMART; SM00504; Ubox; 1.
DR   SUPFAM; SSF48371; SSF48371; 1.
DR   PROSITE; PS51698; U_BOX; 1.
PE   2: Evidence at transcript level;
KW   Reference proteome; Repeat; Transferase; Ubl conjugation pathway.
FT   CHAIN           1..420
FT                   /note="U-box domain-containing protein 27"
FT                   /id="PRO_0000322171"
FT   DOMAIN          9..83
FT                   /note="U-box"
FT   REPEAT          130..178
FT                   /note="ARM 1"
FT   REPEAT          184..225
FT                   /note="ARM 2"
FT   REPEAT          228..268
FT                   /note="ARM 3"
FT   REPEAT          270..313
FT                   /note="ARM 4"
SQ   SEQUENCE   420 AA;  46767 MW;  22D0D4ABB3FFE31C CRC64;
     MRKDDLCITV PTFFRCPISL DVMKSPVSLC TGVTYDRASI QRWLDGGNNT CPATMQILQN
     KDFIPNRTLQ RLIEIWSDSV RRRTCVESAE LAAPTRDEIA DAIDRVKIEK EERDDREVLS
     KIVRFGRESD DNRGFLAGKD DFVKLLVDLI NQVDFETTSA AKSLVVQEAV KILSTIRSKV
     SDRRRFSNLI LTNGRDRLSV IVYLFKTGNV ELKIDCAGLL EFIAVDAESK LLIAERDGLI
     TELMKSISKD SDLSLIESSL SCLIAISSPK RVKLNLLREK LIGDVTKLLS DSTSSLSVSV
     TEKCLKLLEI LASTKEGRSE ICGGDGECLK TVVKKLMKVS TAATEHAVTV LWSVSYLFKE
     DKALEAVTSV NGVTKILLLL QSNCSPAVRR MLTDLLKVFK VNSRSCLSAY DTKTTHIMPF