PUB29_ARATH
ID PUB29_ARATH Reviewed; 415 AA.
AC Q9LSA6; Q8GXX8;
DT 26-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 25-MAY-2022, entry version 127.
DE RecName: Full=U-box domain-containing protein 29;
DE EC=2.3.2.27;
DE AltName: Full=Plant U-box protein 29;
DE AltName: Full=RING-type E3 ubiquitin transferase PUB29 {ECO:0000305};
GN Name=PUB29; OrderedLocusNames=At3g18710; ORFNames=MVE11.7;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10819329; DOI=10.1093/dnares/7.2.131;
RA Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence
RT features of the regions of 4,504,864 bp covered by sixty P1 and TAC
RT clones.";
RL DNA Res. 7:131-135(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=11910074; DOI=10.1126/science.1071006;
RA Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T.,
RA Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y.,
RA Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K.,
RA Shinagawa A., Shinozaki K.;
RT "Functional annotation of a full-length Arabidopsis cDNA collection.";
RL Science 296:141-145(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP GENE FAMILY ORGANIZATION, AND NOMENCLATURE.
RX PubMed=11495788; DOI=10.1016/s1360-1385(01)01960-4;
RA Azevedo C., Santos-Rosa M.J., Shirasu K.;
RT "The U-box protein family in plants.";
RL Trends Plant Sci. 6:354-358(2001).
RN [6]
RP GENE FAMILY ORGANIZATION.
RX PubMed=14657406; DOI=10.1104/pp.103.029553;
RA Mudgil Y., Shiu S.-H., Stone S.L., Salt J.N., Goring D.R.;
RT "A large complement of the predicted Arabidopsis ARM repeat proteins are
RT members of the U-box E3 ubiquitin ligase family.";
RL Plant Physiol. 134:59-66(2004).
RN [7]
RP INTERACTION WITH SD129 AND SD25.
RX PubMed=18552232; DOI=10.1104/pp.108.123380;
RA Samuel M.A., Mudgil Y., Salt J.N., Delmas F., Ramachandran S., Chilelli A.,
RA Goring D.R.;
RT "Interactions between the S-domain receptor kinases and AtPUB-ARM E3
RT ubiquitin ligases suggest a conserved signaling pathway in Arabidopsis.";
RL Plant Physiol. 147:2084-2095(2008).
CC -!- FUNCTION: Functions as an E3 ubiquitin ligase. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine +
CC [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.;
CC EC=2.3.2.27;
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC -!- SUBUNIT: Binds to SD129 and SD25.
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DR EMBL; AB026654; BAB01797.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE76134.1; -; Genomic_DNA.
DR EMBL; AK117976; BAC42613.1; -; mRNA.
DR EMBL; BT005359; AAO63423.1; -; mRNA.
DR RefSeq; NP_188501.1; NM_112757.3.
DR AlphaFoldDB; Q9LSA6; -.
DR SMR; Q9LSA6; -.
DR BioGRID; 6735; 4.
DR IntAct; Q9LSA6; 3.
DR STRING; 3702.AT3G18710.1; -.
DR PaxDb; Q9LSA6; -.
DR PRIDE; Q9LSA6; -.
DR EnsemblPlants; AT3G18710.1; AT3G18710.1; AT3G18710.
DR GeneID; 821402; -.
DR Gramene; AT3G18710.1; AT3G18710.1; AT3G18710.
DR KEGG; ath:AT3G18710; -.
DR Araport; AT3G18710; -.
DR TAIR; locus:2093974; AT3G18710.
DR eggNOG; ENOG502QTKN; Eukaryota.
DR HOGENOM; CLU_006348_1_1_1; -.
DR InParanoid; Q9LSA6; -.
DR OMA; WISFDAK; -.
DR OrthoDB; 989243at2759; -.
DR PhylomeDB; Q9LSA6; -.
DR UniPathway; UPA00143; -.
DR PRO; PR:Q9LSA6; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q9LSA6; baseline and differential.
DR Genevisible; Q9LSA6; AT.
DR GO; GO:0009506; C:plasmodesma; HDA:TAIR.
DR GO; GO:0070696; F:transmembrane receptor protein serine/threonine kinase binding; IPI:UniProtKB.
DR GO; GO:0061630; F:ubiquitin protein ligase activity; IEA:InterPro.
DR GO; GO:0004842; F:ubiquitin-protein transferase activity; IDA:TAIR.
DR GO; GO:0016567; P:protein ubiquitination; IDA:TAIR.
DR CDD; cd16664; RING-Ubox_PUB; 1.
DR Gene3D; 1.25.10.10; -; 1.
DR Gene3D; 3.30.40.10; -; 1.
DR InterPro; IPR011989; ARM-like.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR045185; PUB22/23/24-like.
DR InterPro; IPR045210; RING-Ubox_PUB.
DR InterPro; IPR003613; Ubox_domain.
DR InterPro; IPR013083; Znf_RING/FYVE/PHD.
DR PANTHER; PTHR22849; PTHR22849; 1.
DR Pfam; PF04564; U-box; 1.
DR SMART; SM00504; Ubox; 1.
DR SUPFAM; SSF48371; SSF48371; 1.
DR PROSITE; PS51698; U_BOX; 1.
PE 1: Evidence at protein level;
KW Reference proteome; Repeat; Transferase; Ubl conjugation pathway.
FT CHAIN 1..415
FT /note="U-box domain-containing protein 29"
FT /id="PRO_0000322173"
FT DOMAIN 11..85
FT /note="U-box"
FT REPEAT 221..263
FT /note="ARM 1"
FT REPEAT 265..307
FT /note="ARM 2"
FT CONFLICT 220
FT /note="A -> V (in Ref. 3; AAO63423 and 4; BAC42613)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 415 AA; 46555 MW; 6A6837ABAA6EC450 CRC64;
MGRDETETYI TVPSFFKCPI SLDVMRSPVS LCTGVTYDRA SIQRWLDGGN NTCPATMQLL
KTKDFVPNLT LQRLINIWSD SIGRRHNGDS PVLNPPSGRE VPTKEEVNVL LERLMSLENL
MKIVRFVKDS DSNREFLSKK MEFVPMLVDI IRTKKTKIEL VIMAIRILDS IKVDRERLSN
LMLANDGGDC LTAILLAIQR GNLESKIESV RVLDWISFDA KSKLMIAERD GVLTEMMKSI
SITESSDPSL IEASLSFLIT ISKSKRVRSK LIAAKAITKI KDILLTETLT NVAVTEKSLK
LLETLSSKRE GRLEICGDDN GRCVEGVVKK LLKVSTTATE HAVTILWCLC YVFREDKTVE
ETVERSNGVT KLLVVIQSNC SAMVRQMAKD LIKVLKFNSS ALAAYETKTT HIMPF
