PUB2_ARATH
ID PUB2_ARATH Reviewed; 707 AA.
AC Q5XEZ8; Q0WNF4; Q9FN17;
DT 26-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT 23-NOV-2004, sequence version 1.
DT 03-AUG-2022, entry version 117.
DE RecName: Full=U-box domain-containing protein 2;
DE EC=2.3.2.27;
DE AltName: Full=Plant U-box protein 2;
DE AltName: Full=RING-type E3 ubiquitin transferase PUB2 {ECO:0000305};
GN Name=PUB2; OrderedLocusNames=At5g67340; ORFNames=K8K14.6;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9501997; DOI=10.1093/dnares/4.6.401;
RA Nakamura Y., Sato S., Kaneko T., Kotani H., Asamizu E., Miyajima N.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. III. Sequence
RT features of the regions of 1,191,918 bp covered by seventeen physically
RT assigned P1 clones.";
RL DNA Res. 4:401-414(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Shinn P., Chen H., Cheuk R.F., Kim C.J., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 519-707.
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP GENE FAMILY ORGANIZATION, AND NOMENCLATURE.
RX PubMed=11495788; DOI=10.1016/s1360-1385(01)01960-4;
RA Azevedo C., Santos-Rosa M.J., Shirasu K.;
RT "The U-box protein family in plants.";
RL Trends Plant Sci. 6:354-358(2001).
RN [6]
RP GENE FAMILY ORGANIZATION.
RX PubMed=14657406; DOI=10.1104/pp.103.029553;
RA Mudgil Y., Shiu S.-H., Stone S.L., Salt J.N., Goring D.R.;
RT "A large complement of the predicted Arabidopsis ARM repeat proteins are
RT members of the U-box E3 ubiquitin ligase family.";
RL Plant Physiol. 134:59-66(2004).
CC -!- FUNCTION: Functions as an E3 ubiquitin ligase. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine +
CC [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.;
CC EC=2.3.2.27;
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAB09019.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AB007645; BAB09019.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002688; AED98330.1; -; Genomic_DNA.
DR EMBL; CP002688; ANM70639.1; -; Genomic_DNA.
DR EMBL; BT015818; AAU94381.1; -; mRNA.
DR EMBL; BT020206; AAV59272.1; -; mRNA.
DR EMBL; AK229487; BAF01345.1; -; mRNA.
DR RefSeq; NP_001332230.1; NM_001345821.1.
DR RefSeq; NP_201535.3; NM_126134.5.
DR AlphaFoldDB; Q5XEZ8; -.
DR SMR; Q5XEZ8; -.
DR STRING; 3702.AT5G67340.1; -.
DR iPTMnet; Q5XEZ8; -.
DR PaxDb; Q5XEZ8; -.
DR PRIDE; Q5XEZ8; -.
DR ProteomicsDB; 226107; -.
DR EnsemblPlants; AT5G67340.1; AT5G67340.1; AT5G67340.
DR EnsemblPlants; AT5G67340.2; AT5G67340.2; AT5G67340.
DR GeneID; 836869; -.
DR Gramene; AT5G67340.1; AT5G67340.1; AT5G67340.
DR Gramene; AT5G67340.2; AT5G67340.2; AT5G67340.
DR KEGG; ath:AT5G67340; -.
DR Araport; AT5G67340; -.
DR TAIR; locus:2158252; AT5G67340.
DR eggNOG; KOG0167; Eukaryota.
DR HOGENOM; CLU_006348_5_0_1; -.
DR InParanoid; Q5XEZ8; -.
DR OMA; ITHMHDH; -.
DR OrthoDB; 243233at2759; -.
DR PhylomeDB; Q5XEZ8; -.
DR UniPathway; UPA00143; -.
DR PRO; PR:Q5XEZ8; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q5XEZ8; baseline and differential.
DR Genevisible; Q5XEZ8; AT.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0004842; F:ubiquitin-protein transferase activity; IEA:InterPro.
DR CDD; cd16664; RING-Ubox_PUB; 1.
DR Gene3D; 1.25.10.10; -; 2.
DR Gene3D; 3.30.40.10; -; 1.
DR InterPro; IPR011989; ARM-like.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR000225; Armadillo.
DR InterPro; IPR045210; RING-Ubox_PUB.
DR InterPro; IPR003613; Ubox_domain.
DR InterPro; IPR013083; Znf_RING/FYVE/PHD.
DR Pfam; PF00514; Arm; 2.
DR Pfam; PF04564; U-box; 1.
DR SMART; SM00185; ARM; 5.
DR SMART; SM00504; Ubox; 1.
DR SUPFAM; SSF48371; SSF48371; 1.
DR PROSITE; PS50176; ARM_REPEAT; 3.
DR PROSITE; PS51698; U_BOX; 1.
PE 2: Evidence at transcript level;
KW Reference proteome; Repeat; Transferase; Ubl conjugation pathway.
FT CHAIN 1..707
FT /note="U-box domain-containing protein 2"
FT /id="PRO_0000322148"
FT DOMAIN 239..313
FT /note="U-box"
FT REPEAT 453..492
FT /note="ARM 1"
FT REPEAT 494..534
FT /note="ARM 2"
FT REPEAT 536..575
FT /note="ARM 3"
FT REPEAT 577..615
FT /note="ARM 4"
FT REPEAT 617..656
FT /note="ARM 5"
FT CONFLICT 521
FT /note="A -> P (in Ref. 4; BAF01345)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 707 AA; 78120 MW; AC944FF8797F5A92 CRC64;
MMVHMEVSWL RVLLDNISSY LSLSSMDDLS SNPAHKYYTR GEDIGKLIKP VLENLIDSDA
APSELLNNGF EELAQYVDEL REQFQSWQPL STRIFYVLRI ESLASKLRES SLEVFQLLKH
CEQHLPADLI SPSFEECIEL VKLVARDEIS YTIDQALKDQ KKGVGPTSEV LVKIAESTGL
RSNQEILVEG VVLTNMKEDA ELTDNDTEAE YLDGLISLTT QMHEYLSDIK QAQLRCPVRV
PSDFRCSLSL ELMTDPVIVA SGQTFERVFI QKWIDMGLMV CPKTRQALSH TTLTPNFIVR
AFLASWCETN NVYPPDPLEL IHSSEPFPLL VESVRASSSE NGHSESLDAE ELRQVFSRSA
SAPGIVSEVV CKTKRNNNAA ADRSLTRSNT PWKFPEERHW RHPGIIPATV RETGSSSSIE
TEVKKLIDDL KSSSLDTQRE ATARIRILAR NSTDNRIVIA RCEAIPSLVS LLYSTDERIQ
ADAVTCLLNL SINDNNKSLI AESGAIVPLI HVLKTGYLEE AKANSAATLF SLSVIEEYKT
EIGEAGAIEP LVDLLGSGSL SGKKDAATAL FNLSIHHENK TKVIEAGAVR YLVELMDPAF
GMVEKAVVVL ANLATVREGK IAIGEEGGIP VLVEVVELGS ARGKENATAA LLQLCTHSPK
FCNNVIREGV IPPLVALTKS GTARGKEKAQ NLLKYFKAHR QSNQRRG
