PUB30_ARATH
ID PUB30_ARATH Reviewed; 448 AA.
AC Q058P4; Q8LFR0; Q9M2X7;
DT 26-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT 14-NOV-2006, sequence version 1.
DT 03-AUG-2022, entry version 108.
DE RecName: Full=U-box domain-containing protein 30;
DE EC=2.3.2.27;
DE AltName: Full=Plant U-box protein 30;
DE AltName: Full=RING-type E3 ubiquitin transferase PUB30 {ECO:0000305};
GN Name=PUB30; OrderedLocusNames=At3g49810; ORFNames=T16K5.160;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130713; DOI=10.1038/35048706;
RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL Nature 408:820-822(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Bautista V.R., Kim C.J., Chen H., Quinitio C., Ecker J.R.;
RT "Arabidopsis ORF Clone.";
RL Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP GENE FAMILY ORGANIZATION, AND NOMENCLATURE.
RX PubMed=11495788; DOI=10.1016/s1360-1385(01)01960-4;
RA Azevedo C., Santos-Rosa M.J., Shirasu K.;
RT "The U-box protein family in plants.";
RL Trends Plant Sci. 6:354-358(2001).
RN [6]
RP GENE FAMILY ORGANIZATION.
RX PubMed=14657406; DOI=10.1104/pp.103.029553;
RA Mudgil Y., Shiu S.-H., Stone S.L., Salt J.N., Goring D.R.;
RT "A large complement of the predicted Arabidopsis ARM repeat proteins are
RT members of the U-box E3 ubiquitin ligase family.";
RL Plant Physiol. 134:59-66(2004).
CC -!- FUNCTION: Functions as an E3 ubiquitin ligase. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine +
CC [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.;
CC EC=2.3.2.27;
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC -!- INTERACTION:
CC Q058P4; Q17TI5: BRX; NbExp=3; IntAct=EBI-4442587, EBI-4426649;
CC Q058P4; O23160: MYB73; NbExp=3; IntAct=EBI-4442587, EBI-25506855;
CC -!- SEQUENCE CAUTION:
CC Sequence=CAB66919.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AL132965; CAB66919.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002686; AEE78593.1; -; Genomic_DNA.
DR EMBL; BT029174; ABJ17109.1; -; mRNA.
DR EMBL; AY084697; AAM61258.1; -; mRNA.
DR PIR; T46047; T46047.
DR RefSeq; NP_566927.1; NM_114841.4.
DR AlphaFoldDB; Q058P4; -.
DR SMR; Q058P4; -.
DR BioGRID; 9461; 5.
DR IntAct; Q058P4; 5.
DR STRING; 3702.AT3G49810.1; -.
DR PaxDb; Q058P4; -.
DR PRIDE; Q058P4; -.
DR ProteomicsDB; 226108; -.
DR EnsemblPlants; AT3G49810.1; AT3G49810.1; AT3G49810.
DR GeneID; 824143; -.
DR Gramene; AT3G49810.1; AT3G49810.1; AT3G49810.
DR KEGG; ath:AT3G49810; -.
DR Araport; AT3G49810; -.
DR TAIR; locus:2097390; AT3G49810.
DR eggNOG; ENOG502QTEJ; Eukaryota.
DR HOGENOM; CLU_006348_1_2_1; -.
DR InParanoid; Q058P4; -.
DR OMA; THQSVTK; -.
DR OrthoDB; 649059at2759; -.
DR PhylomeDB; Q058P4; -.
DR UniPathway; UPA00143; -.
DR PRO; PR:Q058P4; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q058P4; baseline and differential.
DR Genevisible; Q058P4; AT.
DR GO; GO:0061630; F:ubiquitin protein ligase activity; IDA:TAIR.
DR GO; GO:0071472; P:cellular response to salt stress; IEP:TAIR.
DR GO; GO:1901001; P:negative regulation of response to salt stress; IMP:TAIR.
DR CDD; cd16664; RING-Ubox_PUB; 1.
DR Gene3D; 1.25.10.10; -; 1.
DR Gene3D; 3.30.40.10; -; 1.
DR InterPro; IPR011989; ARM-like.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR045185; PUB22/23/24-like.
DR InterPro; IPR045210; RING-Ubox_PUB.
DR InterPro; IPR003613; Ubox_domain.
DR InterPro; IPR013083; Znf_RING/FYVE/PHD.
DR PANTHER; PTHR22849; PTHR22849; 1.
DR Pfam; PF04564; U-box; 1.
DR SMART; SM00504; Ubox; 1.
DR SUPFAM; SSF48371; SSF48371; 1.
DR PROSITE; PS51698; U_BOX; 1.
PE 1: Evidence at protein level;
KW Reference proteome; Repeat; Transferase; Ubl conjugation pathway.
FT CHAIN 1..448
FT /note="U-box domain-containing protein 30"
FT /id="PRO_0000322174"
FT DOMAIN 63..137
FT /note="U-box"
FT REPEAT 179..219
FT /note="ARM 1"
FT REPEAT 221..260
FT /note="ARM 2"
FT CONFLICT 176
FT /note="I -> V (in Ref. 4; AAM61258)"
FT /evidence="ECO:0000305"
FT CONFLICT 325
FT /note="P -> Q (in Ref. 4; AAM61258)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 448 AA; 48994 MW; 4F03CC1D56C8C863 CRC64;
MPMFQPLKRD GLIGFEGGGD GQVLDLDTAV KDGVLGGVNG GGVGVVDEKL DLKKMIKELD
LQDIPSVFIC PISLEPMQDP VTLCTGQTYE RLNIHKWFNL GHLTCPTTMQ ELWDDTVTPN
KTLHHLIYTW FSQKYVLMKK RSEDVQGRAI EILGTLKKAK GQARVHALSE LKQIVIAHLM
ARKTVVEEGG VSVISSLLGP FTSHAVGSEV VAILVSLDLD SDSKSGLMQP AKVSLIVDML
NDGSNETKIN CARLIRGLVE EKGFRAELVS SHSLLVGLMR LVKDKRHRNG VSPALRLLKP
ISVHKQVRSL MVSIGAVPQL VDILPSLDPE CLELALFVLD ALCTDVEGRV AVKDSANTIP
YTVRVLMRVS ENCTNYALSI LWSVCKLAPE ECSPLAVEVG LAAKLLLVIQ SGCDAALKQR
SAELLKLCSL HYSDTMFISK CKLTRTIQ
