PUB31_ARATH
ID PUB31_ARATH Reviewed; 444 AA.
AC Q9FHN9; O49529; Q8W4M3;
DT 26-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 117.
DE RecName: Full=U-box domain-containing protein 31;
DE EC=2.3.2.27;
DE AltName: Full=Plant U-box protein 31;
DE AltName: Full=RING-type E3 ubiquitin transferase PUB31 {ECO:0000305};
GN Name=PUB31; OrderedLocusNames=At5g65920; ORFNames=F6H11.10, K14B20.9;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10718197; DOI=10.1093/dnares/7.1.31;
RA Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Kotani H.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. X. Sequence
RT features of the regions of 3,076,755 bp covered by sixty P1 and TAC
RT clones.";
RL DNA Res. 7:31-63(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130714; DOI=10.1038/35048507;
RA Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E.,
RA Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M.,
RA Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.,
RA Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M.,
RA Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L.,
RA O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D.,
RA Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M.,
RA Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L.,
RA Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B.,
RA Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P.,
RA Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M.,
RA Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D.,
RA Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A.,
RA Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I.,
RA Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T.,
RA Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A.,
RA McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U.,
RA Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W.,
RA Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M.,
RA Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S.,
RA Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G.,
RA Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W.,
RA Bevan M., Fransz P.F.;
RT "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.";
RL Nature 408:823-826(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP GENE FAMILY ORGANIZATION, AND NOMENCLATURE.
RX PubMed=11495788; DOI=10.1016/s1360-1385(01)01960-4;
RA Azevedo C., Santos-Rosa M.J., Shirasu K.;
RT "The U-box protein family in plants.";
RL Trends Plant Sci. 6:354-358(2001).
RN [6]
RP GENE FAMILY ORGANIZATION.
RX PubMed=14657406; DOI=10.1104/pp.103.029553;
RA Mudgil Y., Shiu S.-H., Stone S.L., Salt J.N., Goring D.R.;
RT "A large complement of the predicted Arabidopsis ARM repeat proteins are
RT members of the U-box E3 ubiquitin ligase family.";
RL Plant Physiol. 134:59-66(2004).
CC -!- FUNCTION: Functions as an E3 ubiquitin ligase. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine +
CC [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.;
CC EC=2.3.2.27;
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAA16672.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AB018108; BAB11139.1; -; Genomic_DNA.
DR EMBL; AL021684; CAA16672.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002688; AED98123.1; -; Genomic_DNA.
DR EMBL; CP002688; ANM68939.1; -; Genomic_DNA.
DR EMBL; AY062476; AAL32554.1; -; mRNA.
DR EMBL; AY093259; AAM13258.1; -; mRNA.
DR PIR; T05882; T05882.
DR RefSeq; NP_001330653.1; NM_001345727.1.
DR RefSeq; NP_201393.1; NM_125989.4.
DR AlphaFoldDB; Q9FHN9; -.
DR SMR; Q9FHN9; -.
DR STRING; 3702.AT5G65920.1; -.
DR PaxDb; Q9FHN9; -.
DR PRIDE; Q9FHN9; -.
DR EnsemblPlants; AT5G65920.1; AT5G65920.1; AT5G65920.
DR EnsemblPlants; AT5G65920.2; AT5G65920.2; AT5G65920.
DR GeneID; 836721; -.
DR Gramene; AT5G65920.1; AT5G65920.1; AT5G65920.
DR Gramene; AT5G65920.2; AT5G65920.2; AT5G65920.
DR KEGG; ath:AT5G65920; -.
DR Araport; AT5G65920; -.
DR TAIR; locus:2152049; AT5G65920.
DR eggNOG; ENOG502QTEJ; Eukaryota.
DR HOGENOM; CLU_006348_1_2_1; -.
DR InParanoid; Q9FHN9; -.
DR OMA; KESVNTI; -.
DR OrthoDB; 649059at2759; -.
DR PhylomeDB; Q9FHN9; -.
DR UniPathway; UPA00143; -.
DR PRO; PR:Q9FHN9; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9FHN9; baseline and differential.
DR GO; GO:0061630; F:ubiquitin protein ligase activity; IEA:InterPro.
DR CDD; cd16664; RING-Ubox_PUB; 1.
DR Gene3D; 1.25.10.10; -; 1.
DR Gene3D; 3.30.40.10; -; 1.
DR InterPro; IPR011989; ARM-like.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR045185; PUB22/23/24-like.
DR InterPro; IPR045210; RING-Ubox_PUB.
DR InterPro; IPR003613; Ubox_domain.
DR InterPro; IPR013083; Znf_RING/FYVE/PHD.
DR PANTHER; PTHR22849; PTHR22849; 1.
DR Pfam; PF04564; U-box; 1.
DR SMART; SM00504; Ubox; 1.
DR SUPFAM; SSF48371; SSF48371; 2.
DR PROSITE; PS51698; U_BOX; 1.
PE 2: Evidence at transcript level;
KW Reference proteome; Repeat; Transferase; Ubl conjugation pathway.
FT CHAIN 1..444
FT /note="U-box domain-containing protein 31"
FT /id="PRO_0000322175"
FT DOMAIN 59..133
FT /note="U-box"
FT REPEAT 301..340
FT /note="ARM 1"
FT REPEAT 343..382
FT /note="ARM 2"
FT CONFLICT 184
FT /note="E -> G (in Ref. 4; AAL32554/AAM13258)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 444 AA; 48873 MW; 0C2F025F0C714A0B CRC64;
MPMFQPSKNG GFDGHILDLH SAVKDGVLGG GDGKFLVVVT DEKKKLDLKE MISELELPEI
PSVFICPISL EPMQDPVTLC TGQTYERSNI LKWFNIGHCT CPTTMQELWD DLVTPNKTLH
QLIYTWFSQK YVLMKKRSED VQGRAIEILG TLRKAKGKAK VHALSELKQV VMAHAIAKKT
VVDEGGVFVI SSLLSPFTSH AVGSEAIAIL VNLELDSDSK AGLMQPARVS LMVDMLNDGS
IETKINCARL IGRLVEEKGF RAELVSSHSL LVGLMRLVKD RRRRNGVSPA LTLLKSVSVH
KQVRNLLVRI GAVPQLVDVL PCLDVECLES ALFVLDSLCL ESEGRIALKD SVNTIPHTVR
LLMKVSEKCT NYAISILWSV CKLASEECSS LAVEVGLAAK LLLVIQSGCD PALKQRSAEL
LKLCSLHYSD SMFISKCKLT RTIQ
