PUB3_ARATH
ID PUB3_ARATH Reviewed; 760 AA.
AC Q8GWV5; Q9M1S1;
DT 26-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT 26-FEB-2008, sequence version 2.
DT 03-AUG-2022, entry version 117.
DE RecName: Full=U-box domain-containing protein 3;
DE EC=2.3.2.27;
DE AltName: Full=Plant U-box protein 3;
DE AltName: Full=RING-type E3 ubiquitin transferase PUB3 {ECO:0000305};
GN Name=PUB3; OrderedLocusNames=At3g54790; ORFNames=T5N23_150;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130713; DOI=10.1038/35048706;
RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL Nature 408:820-822(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=11910074; DOI=10.1126/science.1071006;
RA Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T.,
RA Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y.,
RA Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K.,
RA Shinagawa A., Shinozaki K.;
RT "Functional annotation of a full-length Arabidopsis cDNA collection.";
RL Science 296:141-145(2002).
RN [4]
RP GENE FAMILY ORGANIZATION, AND NOMENCLATURE.
RX PubMed=11495788; DOI=10.1016/s1360-1385(01)01960-4;
RA Azevedo C., Santos-Rosa M.J., Shirasu K.;
RT "The U-box protein family in plants.";
RL Trends Plant Sci. 6:354-358(2001).
RN [5]
RP GENE FAMILY ORGANIZATION.
RX PubMed=14657406; DOI=10.1104/pp.103.029553;
RA Mudgil Y., Shiu S.-H., Stone S.L., Salt J.N., Goring D.R.;
RT "A large complement of the predicted Arabidopsis ARM repeat proteins are
RT members of the U-box E3 ubiquitin ligase family.";
RL Plant Physiol. 134:59-66(2004).
CC -!- FUNCTION: Functions as an E3 ubiquitin ligase. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine +
CC [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.;
CC EC=2.3.2.27;
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=1;
CC Comment=A number of isoforms are produced. According to EST
CC sequences.;
CC Name=1;
CC IsoId=Q8GWV5-1; Sequence=Displayed;
CC -!- SEQUENCE CAUTION:
CC Sequence=CAB77599.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AL138650; CAB77599.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002686; AEE79287.1; -; Genomic_DNA.
DR EMBL; CP002686; ANM63723.1; -; Genomic_DNA.
DR EMBL; AK118613; BAC43212.1; -; mRNA.
DR PIR; T47638; T47638.
DR RefSeq; NP_001325795.1; NM_001339699.1. [Q8GWV5-1]
DR RefSeq; NP_191039.2; NM_115336.5. [Q8GWV5-1]
DR AlphaFoldDB; Q8GWV5; -.
DR SMR; Q8GWV5; -.
DR STRING; 3702.AT3G54790.1; -.
DR PaxDb; Q8GWV5; -.
DR PRIDE; Q8GWV5; -.
DR ProteomicsDB; 226069; -. [Q8GWV5-1]
DR EnsemblPlants; AT3G54790.1; AT3G54790.1; AT3G54790. [Q8GWV5-1]
DR EnsemblPlants; AT3G54790.3; AT3G54790.3; AT3G54790. [Q8GWV5-1]
DR GeneID; 824644; -.
DR Gramene; AT3G54790.1; AT3G54790.1; AT3G54790. [Q8GWV5-1]
DR Gramene; AT3G54790.3; AT3G54790.3; AT3G54790. [Q8GWV5-1]
DR KEGG; ath:AT3G54790; -.
DR Araport; AT3G54790; -.
DR TAIR; locus:2102455; AT3G54790.
DR eggNOG; KOG0167; Eukaryota.
DR InParanoid; Q8GWV5; -.
DR OMA; PSDETIC; -.
DR OrthoDB; 273203at2759; -.
DR PhylomeDB; Q8GWV5; -.
DR UniPathway; UPA00143; -.
DR PRO; PR:Q8GWV5; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q8GWV5; baseline and differential.
DR Genevisible; Q8GWV5; AT.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0004842; F:ubiquitin-protein transferase activity; IEA:InterPro.
DR CDD; cd16664; RING-Ubox_PUB; 1.
DR Gene3D; 1.25.10.10; -; 2.
DR Gene3D; 3.30.40.10; -; 1.
DR InterPro; IPR011989; ARM-like.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR000225; Armadillo.
DR InterPro; IPR045210; RING-Ubox_PUB.
DR InterPro; IPR003613; Ubox_domain.
DR InterPro; IPR013083; Znf_RING/FYVE/PHD.
DR Pfam; PF00514; Arm; 2.
DR Pfam; PF04564; U-box; 1.
DR SMART; SM00185; ARM; 5.
DR SMART; SM00504; Ubox; 1.
DR SUPFAM; SSF48371; SSF48371; 1.
DR PROSITE; PS50176; ARM_REPEAT; 3.
DR PROSITE; PS51698; U_BOX; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Coiled coil; Reference proteome; Repeat; Transferase;
KW Ubl conjugation pathway.
FT CHAIN 1..760
FT /note="U-box domain-containing protein 3"
FT /id="PRO_0000322149"
FT DOMAIN 237..311
FT /note="U-box"
FT REPEAT 504..543
FT /note="ARM 1"
FT REPEAT 545..584
FT /note="ARM 2"
FT REPEAT 586..626
FT /note="ARM 3"
FT REPEAT 628..666
FT /note="ARM 4"
FT REPEAT 668..707
FT /note="ARM 5"
FT REGION 424..448
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 146..217
FT /evidence="ECO:0000255"
FT CONFLICT 66
FT /note="C -> R (in Ref. 3; BAC43212)"
FT /evidence="ECO:0000305"
FT CONFLICT 557
FT /note="I -> V (in Ref. 3; BAC43212)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 760 AA; 83779 MW; 997BE2C227DF9785 CRC64;
MDPVPVRCLL NSISRYLHLV ACQTIRFNPI QTCIGNMVLL LKLLKPLLDE VVDCKIPSDD
CLYKGCEDLD SVVNQAREFL EDWSPKLSKL FGVFQCEVLL GKVQTCSLEI SRILLQLSQS
SPVTSSVQSV ERCVQETESF KQEGTLMELM ENALRNQKDD ITSLDNNHLE SIIQMLGLIS
NQDLLKESIT VEKERIRSQA SKSEEDMEQT EQLIELVLCI REHMLKTEFL EVAKGISIPP
YFRCPLSTEL MLDPVIVASG QTFDRTSIKK WLDNGLAVCP RTRQVLTHQE LIPNYTVKAM
IASWLEANRI NLATNSCHQY DGGDASSMAN NMGSQDFNRT ESFRFSLRSS SLTSRSSLET
GNGFEKLKIN VSASLCGESQ SKDLEIFELL SPGQSYTHSR SESVCSVVSS VDYVPSVTHE
TESILGNHQS SSEMSPKKNL ESSNNVNHEH SAAKTYECSV HDLDDSGTMT TSHTIKLVED
LKSGSNKVKT AAAAEIRHLT INSIENRVHI GRCGAITPLL SLLYSEEKLT QEHAVTALLN
LSISELNKAM IVEVGAIEPL VHVLNTGNDR AKENSAASLF SLSVLQVNRE RIGQSNAAIQ
ALVNLLGKGT FRGKKDAASA LFNLSITHDN KARIVQAKAV KYLVELLDPD LEMVDKAVAL
LANLSAVGEG RQAIVREGGI PLLVETVDLG SQRGKENAAS VLLQLCLNSP KFCTLVLQEG
AIPPLVALSQ SGTQRAKEKA QQLLSHFRNQ RDARMKKGRS
