ID   PUB40_ARATH             Reviewed;         550 AA.
AC   Q9FL17;
DT   26-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT   31-MAY-2011, sequence version 2.
DT   03-AUG-2022, entry version 132.
DE   RecName: Full=U-box domain-containing protein 40;
DE            EC=2.3.2.27;
DE   AltName: Full=Plant U-box protein 40;
DE   AltName: Full=RING-type E3 ubiquitin transferase PUB40 {ECO:0000305};
GN   Name=PUB40; OrderedLocusNames=At5g40140; ORFNames=MSN9.4;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=9679202; DOI=10.1093/dnares/5.2.131;
RA   Kaneko T., Kotani H., Nakamura Y., Sato S., Asamizu E., Miyajima N.,
RA   Tabata S.;
RT   "Structural analysis of Arabidopsis thaliana chromosome 5. V. Sequence
RT   features of the regions of 1,381,565 bp covered by twenty one physically
RT   assigned P1 and TAC clones.";
RL   DNA Res. 5:131-145(1998).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   GENE FAMILY ORGANIZATION.
RX   PubMed=14657406; DOI=10.1104/pp.103.029553;
RA   Mudgil Y., Shiu S.-H., Stone S.L., Salt J.N., Goring D.R.;
RT   "A large complement of the predicted Arabidopsis ARM repeat proteins are
RT   members of the U-box E3 ubiquitin ligase family.";
RL   Plant Physiol. 134:59-66(2004).
CC   -!- FUNCTION: Functions as an E3 ubiquitin ligase. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine +
CC         [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC         cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.;
CC         EC=2.3.2.27;
CC   -!- PATHWAY: Protein modification; protein ubiquitination.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=BAB10895.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR   EMBL; AB010699; BAB10895.1; ALT_INIT; Genomic_DNA.
DR   EMBL; CP002688; AED94512.1; -; Genomic_DNA.
DR   RefSeq; NP_198830.1; NM_123377.2.
DR   AlphaFoldDB; Q9FL17; -.
DR   SMR; Q9FL17; -.
DR   STRING; 3702.AT5G40140.1; -.
DR   iPTMnet; Q9FL17; -.
DR   PaxDb; Q9FL17; -.
DR   PRIDE; Q9FL17; -.
DR   ProteomicsDB; 226247; -.
DR   EnsemblPlants; AT5G40140.1; AT5G40140.1; AT5G40140.
DR   GeneID; 834011; -.
DR   Gramene; AT5G40140.1; AT5G40140.1; AT5G40140.
DR   KEGG; ath:AT5G40140; -.
DR   Araport; AT5G40140; -.
DR   TAIR; locus:2173742; AT5G40140.
DR   eggNOG; ENOG502QPJU; Eukaryota.
DR   HOGENOM; CLU_006348_7_0_1; -.
DR   InParanoid; Q9FL17; -.
DR   OMA; MEFQEAV; -.
DR   OrthoDB; 425663at2759; -.
DR   UniPathway; UPA00143; -.
DR   PRO; PR:Q9FL17; -.
DR   Proteomes; UP000006548; Chromosome 5.
DR   ExpressionAtlas; Q9FL17; baseline and differential.
DR   Genevisible; Q9FL17; AT.
DR   GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR   GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR   GO; GO:0061630; F:ubiquitin protein ligase activity; IDA:TAIR.
DR   Gene3D; 1.25.10.10; -; 1.
DR   Gene3D; 3.30.40.10; -; 1.
DR   InterPro; IPR011989; ARM-like.
DR   InterPro; IPR016024; ARM-type_fold.
DR   InterPro; IPR000225; Armadillo.
DR   InterPro; IPR003613; Ubox_domain.
DR   InterPro; IPR013083; Znf_RING/FYVE/PHD.
DR   Pfam; PF00514; Arm; 1.
DR   Pfam; PF04564; U-box; 1.
DR   SMART; SM00185; ARM; 4.
DR   SMART; SM00504; Ubox; 1.
DR   SUPFAM; SSF48371; SSF48371; 1.
DR   PROSITE; PS50176; ARM_REPEAT; 1.
DR   PROSITE; PS51698; U_BOX; 1.
PE   2: Evidence at transcript level;
KW   Reference proteome; Repeat; Transferase; Ubl conjugation pathway.
FT   CHAIN           1..550
FT                   /note="U-box domain-containing protein 40"
FT                   /id="PRO_0000322180"
FT   DOMAIN          57..127
FT                   /note="U-box"
FT   REPEAT          260..299
FT                   /note="ARM 1"
FT   REPEAT          301..340
FT                   /note="ARM 2"
FT   REPEAT          342..381
FT                   /note="ARM 3"
FT   REPEAT          383..420
FT                   /note="ARM 4"
FT   REPEAT          422..464
FT                   /note="ARM 5"
FT   REGION          19..55
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        33..55
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   550 AA;  60218 MW;  A00D9913A537B5D6 CRC64;
     MEIQRPVINL MVLHSQHDKS DNLSRRESLA GKSKWRTSLS RSSSSSSSNN NSPTKTEIPA
     EFLCPISGSL MADPIIVSSG HSYERACVIA CKTLGFTPTP PPDFSTVIPN LALKSAIHSW
     CERRCFPPPK PLNSAAAEKL ILALMEKKPQ RRKVSVSEKE LIQAIRDKPS VRLNHAATEL
     DRRPNYFNSS SDESIASSSR TLQLTTKPSC FSSPSSGEIE SLEPNLTPEE EALLTKLKSN
     RISEIEEALI SIRRITRIDE SSRISLCTTR VISALKSLIV SRYATVQVNV TAVLVNLSLE
     KSNKVKIVRS GIVPPLIDVL KCGSVEAQEH SAGVIFSLAL EDENKTAIGV LGGLEPLLHL
     IRVGTELTRH DSALALYHLS LVQSNRGKLV KLGAVQMLLG MVSLGQMIGR VLLILCNMAS
     CPVSRPALLD SGGVECMVGV LRRDREVNES TRESCVAVLY GLSHDGGLRF KGLAMAANAV
     EELVKVERSG RERAKQKARR VLEVLRAKIE DDDLVENEEI DWEELLNSGD VSRSRCRLGG
     EKSCVNSAEF