PUB42_ARATH
ID PUB42_ARATH Reviewed; 1033 AA.
AC Q9CAA7;
DT 26-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 25-MAY-2022, entry version 142.
DE RecName: Full=Putative U-box domain-containing protein 42;
DE EC=2.3.2.27;
DE AltName: Full=Plant U-box protein 42;
DE AltName: Full=RING-type E3 ubiquitin transferase PUB42 {ECO:0000305};
GN Name=PUB42; OrderedLocusNames=At1g68940; ORFNames=T6L1.12;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP GENE FAMILY ORGANIZATION.
RX PubMed=14657406; DOI=10.1104/pp.103.029553;
RA Mudgil Y., Shiu S.-H., Stone S.L., Salt J.N., Goring D.R.;
RT "A large complement of the predicted Arabidopsis ARM repeat proteins are
RT members of the U-box E3 ubiquitin ligase family.";
RL Plant Physiol. 134:59-66(2004).
CC -!- FUNCTION: Functions as an E3 ubiquitin ligase. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine +
CC [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.;
CC EC=2.3.2.27;
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=1;
CC Comment=A number of isoforms are produced. According to EST
CC sequences.;
CC Name=1;
CC IsoId=Q9CAA7-1; Sequence=Displayed;
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DR EMBL; AC011665; AAG51587.1; -; Genomic_DNA.
DR EMBL; CP002684; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR PIR; A96714; A96714.
DR AlphaFoldDB; Q9CAA7; -.
DR STRING; 3702.AT1G68940.3; -.
DR iPTMnet; Q9CAA7; -.
DR PaxDb; Q9CAA7; -.
DR PeptideAtlas; Q9CAA7; -.
DR EnsemblPlants; AT1G68940.1; AT1G68940.1; AT1G68940.
DR Gramene; AT1G68940.1; AT1G68940.1; AT1G68940.
DR Araport; AT1G68940; -.
DR eggNOG; KOG0167; Eukaryota.
DR eggNOG; KOG1072; Eukaryota.
DR HOGENOM; CLU_004912_0_0_1; -.
DR InParanoid; Q9CAA7; -.
DR PhylomeDB; Q9CAA7; -.
DR UniPathway; UPA00143; -.
DR PRO; PR:Q9CAA7; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9CAA7; differential.
DR Genevisible; Q9CAA7; AT.
DR GO; GO:0004842; F:ubiquitin-protein transferase activity; IEA:InterPro.
DR CDD; cd16664; RING-Ubox_PUB; 1.
DR Gene3D; 1.25.10.10; -; 3.
DR Gene3D; 3.30.40.10; -; 1.
DR InterPro; IPR011989; ARM-like.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR000225; Armadillo.
DR InterPro; IPR045210; RING-Ubox_PUB.
DR InterPro; IPR003613; Ubox_domain.
DR InterPro; IPR013083; Znf_RING/FYVE/PHD.
DR Pfam; PF04564; U-box; 1.
DR SMART; SM00185; ARM; 6.
DR SMART; SM00504; Ubox; 1.
DR SUPFAM; SSF48371; SSF48371; 2.
DR PROSITE; PS51698; U_BOX; 1.
PE 3: Inferred from homology;
KW Alternative splicing; Reference proteome; Repeat; Transferase;
KW Ubl conjugation pathway.
FT CHAIN 1..1033
FT /note="Putative U-box domain-containing protein 42"
FT /id="PRO_0000322182"
FT DOMAIN 245..322
FT /note="U-box"
FT REPEAT 483..522
FT /note="ARM 1"
FT REPEAT 523..562
FT /note="ARM 2"
FT REPEAT 564..608
FT /note="ARM 3"
FT REPEAT 610..659
FT /note="ARM 4"
FT REPEAT 665..704
FT /note="ARM 5"
FT REGION 145..226
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 145..159
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 174..226
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1033 AA; 114846 MW; 74B2D85B43BF83D6 CRC64;
MEIQTAESNV ICSIDIFESL SDSVDVAKKL VEKSQESNEA ESTTDLRSIE AGFEGVVKQM
GETLQSIPES TFDEEEYIGV VIQSLSNEMQ NATIGDGSKS EMINNGQQKI SAKHTPDIVS
EQMEEDLYPT DPEFSYESYM MYSESQSQMT DIPDIPSKST DVSRQRKHGN HSESQSLVTE
IPDIPSQSTN VSSQRKHGNL SKSQSQSTEI PDIPSQSSNA SSQRKYGNLS ESLSMLPQVT
QFMEPPYQAF ICPLTKEIME DPVTTETGVT CERQAVIEWF DSFGNSDEIN CPVTGQKLTT
ELSANVVLKT IIQEWKVRNE AARIKVAHAA LSLGGSESMV IDALRDLQMT CEGKEYNKVQ
VREAGIIQLL DRYLTYRSKD VRFELLKFLR TLADEETDDG KEMIVKTITM SCVIKLLGSS
HQPVRHAAQA LLLELSKSQH ACEKIGTARG AILMLVTAKY NRELDSFASE TSDQILRNLE
KCPENIKQMA ESGLLEPLLG HLAEGSEETQ VAMAAYLVEI DIGHEKKTYV AEKACPALIG
LVQSENIDAR RAAFKALAHI SLYHPNNKIL VEVGIIKIMV EEMFTKRVFS DLMNSRNEAA
TILANILESG LEHETFEVNT HGHTLGSDYF VYNIIHMLKN SSPDDLNIDL IRILLSLSKS
PRAMATIVSV IKETDASFAM IELINNPHDE LGVGALKLLI ALTPYIGHTL SERLCKTRGQ
PENLIQCPVE ANQITEKHAV SAKLLAKLPH QNLTLNLALV NESIVSEILH AIHLIQRSGA
RTSRYATDFL EGLVGILVRF TTTLYEPQMM YLARNHDLTS VFVDLLMKTS SDEVQRLSAT
GLENLSSTTM TLSRPPQPRS TKFMGSLSMP RSFSLRSSKK KQIEICAIHR GVCSAKNTFC
LVEANAITKL LACLQSDKVE VVESALAAIC TLLDDKVEVE KSLSMLSEMN AVQLILNAVK
EHKKESLLQK AFWMIDKFII RGGDKYASEI SQDRMLSGML VSAFHRGDGN TRQMAENILR
RLDKMPSFST YIT
