PUB43_ARATH
ID PUB43_ARATH Reviewed; 811 AA.
AC Q9SFX2;
DT 26-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 25-MAY-2022, entry version 138.
DE RecName: Full=U-box domain-containing protein 43;
DE EC=2.3.2.27;
DE AltName: Full=Plant U-box protein 43;
DE AltName: Full=RING-type E3 ubiquitin transferase PUB43 {ECO:0000305};
GN Name=PUB43; OrderedLocusNames=At1g76390; ORFNames=F15M4.11;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP GENE FAMILY ORGANIZATION.
RX PubMed=14657406; DOI=10.1104/pp.103.029553;
RA Mudgil Y., Shiu S.-H., Stone S.L., Salt J.N., Goring D.R.;
RT "A large complement of the predicted Arabidopsis ARM repeat proteins are
RT members of the U-box E3 ubiquitin ligase family.";
RL Plant Physiol. 134:59-66(2004).
CC -!- FUNCTION: Functions as an E3 ubiquitin ligase. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine +
CC [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.;
CC EC=2.3.2.27;
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC -!- SEQUENCE CAUTION:
CC Sequence=AK226857; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AC012394; AAF16662.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE35833.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE35834.1; -; Genomic_DNA.
DR EMBL; AK226857; -; NOT_ANNOTATED_CDS; mRNA.
DR PIR; E96791; E96791.
DR RefSeq; NP_001077833.1; NM_001084364.2.
DR RefSeq; NP_177765.1; NM_106288.4.
DR AlphaFoldDB; Q9SFX2; -.
DR STRING; 3702.AT1G76390.2; -.
DR iPTMnet; Q9SFX2; -.
DR PaxDb; Q9SFX2; -.
DR PRIDE; Q9SFX2; -.
DR ProteomicsDB; 226249; -.
DR EnsemblPlants; AT1G76390.1; AT1G76390.1; AT1G76390.
DR EnsemblPlants; AT1G76390.2; AT1G76390.2; AT1G76390.
DR GeneID; 843971; -.
DR Gramene; AT1G76390.1; AT1G76390.1; AT1G76390.
DR Gramene; AT1G76390.2; AT1G76390.2; AT1G76390.
DR KEGG; ath:AT1G76390; -.
DR Araport; AT1G76390; -.
DR TAIR; locus:2014584; AT1G76390.
DR eggNOG; KOG0167; Eukaryota.
DR HOGENOM; CLU_004912_0_0_1; -.
DR InParanoid; Q9SFX2; -.
DR OMA; ECRENGQ; -.
DR OrthoDB; 268677at2759; -.
DR PhylomeDB; Q9SFX2; -.
DR UniPathway; UPA00143; -.
DR PRO; PR:Q9SFX2; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9SFX2; baseline and differential.
DR Genevisible; Q9SFX2; AT.
DR GO; GO:0005739; C:mitochondrion; HDA:TAIR.
DR GO; GO:0005886; C:plasma membrane; HDA:TAIR.
DR GO; GO:0004842; F:ubiquitin-protein transferase activity; IEA:InterPro.
DR CDD; cd16664; RING-Ubox_PUB; 1.
DR Gene3D; 1.25.10.10; -; 3.
DR Gene3D; 3.30.40.10; -; 1.
DR InterPro; IPR011989; ARM-like.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR000225; Armadillo.
DR InterPro; IPR045210; RING-Ubox_PUB.
DR InterPro; IPR003613; Ubox_domain.
DR InterPro; IPR013083; Znf_RING/FYVE/PHD.
DR Pfam; PF04564; U-box; 1.
DR SMART; SM00185; ARM; 8.
DR SMART; SM00504; Ubox; 1.
DR SUPFAM; SSF48371; SSF48371; 2.
DR PROSITE; PS50176; ARM_REPEAT; 1.
DR PROSITE; PS51698; U_BOX; 1.
PE 2: Evidence at transcript level;
KW Reference proteome; Repeat; Transferase; Ubl conjugation pathway.
FT CHAIN 1..811
FT /note="U-box domain-containing protein 43"
FT /id="PRO_0000322183"
FT DOMAIN 24..103
FT /note="U-box"
FT REPEAT 136..175
FT /note="ARM 1"
FT REPEAT 178..217
FT /note="ARM 2"
FT REPEAT 220..261
FT /note="ARM 3"
FT REPEAT 263..302
FT /note="ARM 4"
FT REPEAT 303..342
FT /note="ARM 5"
FT REPEAT 344..388
FT /note="ARM 6"
FT REPEAT 399..438
FT /note="ARM 7"
FT REPEAT 444..484
FT /note="ARM 8"
FT REPEAT 489..528
FT /note="ARM 9"
SQ SEQUENCE 811 AA; 89109 MW; F182B561E9A04384 CRC64;
MAGSGSWDGS QSDNSSQFEP GIDNIYEAFI CPLTKQVMHN PVTLENGQTF EREAIEKWFQ
ECRENGQPLS CPITSKELSI TDLSPSIALR NTIEEWRARN DALKLDIARQ SLYLGNAETN
ILLALKNVRE ICRNIRKIRQ RVCNPQLVRL ITDMLKSSSH EVRCKALQTL QVVVEGDEES
KAIVAEGDTV RTIVKFLSQE PSKGREAAVS VLFELSKSEA LCEKIGSIHG AIILLVGLTS
SKSENVSTVE KADKTLTNLE RSEENVRQMA INGRLQPLLA KLLEGSPETK VSMAFYLGVL
ALNNDVKVIV AQTVGSSLID LMRTRDMSQR EAALGALNNI SSFEGSAKLL INTGILPPLI
KDLFYVGPNQ LPIRLKEVSA TILANIVNIG YDFDKVPVGP HHQTLVSEEI VENLLQLTSN
TGPEIQGKLL AVLVGLTSCP NSVINVVSAI RNSAAIISLV QFVEIHENDD LRLASIKLLH
NISPHMSEEL ANALRSTVGQ LGSLVSIISE NTPTITEEQA AAAGLLAELP ERDLVLTMRL
LREGAFEKII SKIVGIRQGE IRGIRFERTF LEGLVSILAR ITFALTKETD ATLFCCEKNL
PSLFLDLLQS NSQDNIQRAS ATALENLSLE SKNLTKIPEL PPPTYCVSIF SCLSKPPVVL
GICKIHQGIC SVRESFCLVE GQAVDKLVDL LDHENDKVVG PALAALSTLL EDGLDVVQGV
RLIDEADGIT PILNVLLENR TENLRIRAVW MVERILRIEE IAREVGEEQN VTAALVDAFQ
NADFRTRQIA EKALRHIDKI PNFSGIFTNI G
