PUB45_ARATH
ID PUB45_ARATH Reviewed; 768 AA.
AC Q9C7G1; Q0WLV7;
DT 26-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 25-MAY-2022, entry version 138.
DE RecName: Full=U-box domain-containing protein 45;
DE EC=2.3.2.27;
DE AltName: Full=Plant U-box protein 45;
DE AltName: Full=RING-type E3 ubiquitin transferase PUB45 {ECO:0000305};
GN Name=PUB45; OrderedLocusNames=At1g27910; ORFNames=F13K9.2;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 358-768.
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP GENE FAMILY ORGANIZATION.
RX PubMed=14657406; DOI=10.1104/pp.103.029553;
RA Mudgil Y., Shiu S.-H., Stone S.L., Salt J.N., Goring D.R.;
RT "A large complement of the predicted Arabidopsis ARM repeat proteins are
RT members of the U-box E3 ubiquitin ligase family.";
RL Plant Physiol. 134:59-66(2004).
RN [6]
RP INTERACTION WITH SD129.
RX PubMed=18552232; DOI=10.1104/pp.108.123380;
RA Samuel M.A., Mudgil Y., Salt J.N., Delmas F., Ramachandran S., Chilelli A.,
RA Goring D.R.;
RT "Interactions between the S-domain receptor kinases and AtPUB-ARM E3
RT ubiquitin ligases suggest a conserved signaling pathway in Arabidopsis.";
RL Plant Physiol. 147:2084-2095(2008).
CC -!- FUNCTION: Functions as an E3 ubiquitin ligase. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine +
CC [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.;
CC EC=2.3.2.27;
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC -!- SUBUNIT: Binds to SD129.
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DR EMBL; AC069471; AAG51474.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE30890.1; -; Genomic_DNA.
DR EMBL; AY094469; AAM19837.1; -; mRNA.
DR EMBL; BT002721; AAO11637.1; -; mRNA.
DR EMBL; AK230080; BAF01900.1; -; mRNA.
DR PIR; D86404; D86404.
DR RefSeq; NP_174112.1; NM_102556.5.
DR AlphaFoldDB; Q9C7G1; -.
DR SMR; Q9C7G1; -.
DR BioGRID; 24919; 1.
DR iPTMnet; Q9C7G1; -.
DR PaxDb; Q9C7G1; -.
DR PRIDE; Q9C7G1; -.
DR ProteomicsDB; 226073; -.
DR EnsemblPlants; AT1G27910.1; AT1G27910.1; AT1G27910.
DR GeneID; 839684; -.
DR Gramene; AT1G27910.1; AT1G27910.1; AT1G27910.
DR KEGG; ath:AT1G27910; -.
DR Araport; AT1G27910; -.
DR TAIR; locus:2010424; AT1G27910.
DR eggNOG; KOG0167; Eukaryota.
DR HOGENOM; CLU_006348_4_1_1; -.
DR InParanoid; Q9C7G1; -.
DR OMA; AEMKPLC; -.
DR OrthoDB; 174560at2759; -.
DR PhylomeDB; Q9C7G1; -.
DR UniPathway; UPA00143; -.
DR PRO; PR:Q9C7G1; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9C7G1; baseline and differential.
DR Genevisible; Q9C7G1; AT.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0070696; F:transmembrane receptor protein serine/threonine kinase binding; IPI:UniProtKB.
DR GO; GO:0004842; F:ubiquitin-protein transferase activity; IDA:TAIR.
DR GO; GO:0016567; P:protein ubiquitination; IDA:TAIR.
DR CDD; cd16664; RING-Ubox_PUB; 1.
DR Gene3D; 1.25.10.10; -; 1.
DR Gene3D; 3.30.40.10; -; 1.
DR InterPro; IPR011989; ARM-like.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR000225; Armadillo.
DR InterPro; IPR045210; RING-Ubox_PUB.
DR InterPro; IPR003613; Ubox_domain.
DR InterPro; IPR013083; Znf_RING/FYVE/PHD.
DR Pfam; PF04564; U-box; 1.
DR SMART; SM00185; ARM; 3.
DR SMART; SM00504; Ubox; 1.
DR SUPFAM; SSF48371; SSF48371; 1.
DR PROSITE; PS50176; ARM_REPEAT; 1.
DR PROSITE; PS51698; U_BOX; 1.
PE 1: Evidence at protein level;
KW Reference proteome; Repeat; Transferase; Ubl conjugation pathway.
FT CHAIN 1..768
FT /note="U-box domain-containing protein 45"
FT /id="PRO_0000322185"
FT DOMAIN 278..352
FT /note="U-box"
FT REPEAT 454..497
FT /note="ARM 1"
FT REPEAT 500..540
FT /note="ARM 2"
FT REPEAT 542..579
FT /note="ARM 3"
FT REPEAT 581..620
FT /note="ARM 4"
FT REPEAT 623..662
FT /note="ARM 5"
FT CONFLICT 358..366
FT /note="PESLDLNYW -> IAVWNSDDS (in Ref. 4; BAF01900)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 768 AA; 85576 MW; 56F2E18F6BBCE5F2 CRC64;
MDVNEVEENF FAPGDAKLHG KMCNALSVIY CKIMSIFPSL EAARPRSKSG IQALCSLHVV
LEKVKNILRH CTESSKLYLA ITGDSVVLKF EKAKSSLTDS LRRVEDIVQQ SIGSQLLEIL
MELENTEFSL DPAEKEIGDQ IIGLLQQGGN FESSSDNNEL EVFHQAATRL GITSSRAALT
ERRCLKKLIE RARMEDDKRK ESIVAYLLHL MRKYSKLFRS EIWDDNDSQG SSSLPCSPTI
QGSIDDAHGR AFDRQLSKLS SFNFRSCNNN RRSSQMSVPP EELRCPISLQ LMYDPVIIAS
GQTYERICIE KWFSDGHNTC PKTHQQLSHL CLTPNYCVKA LISSWCEQNG VQVPDGPPES
LDLNYWRLAL SVSESTDTRS AKRVGSCKLK DVKVVPLEES GTIKEEACES EYQEDQVTLV
ERCTELLTTL TDVDTLRKKC RVVEQIRVLL KDDEEARILM GENGCVEALL QFLGSALNEN
NASAQKVGAM ALFNLAVDNN RNKELMLASG IIPLLEEMLC NPHSHGSVTA IYLNLSCLEE
AKPVIGSSLA VPFMVNLLWT ETEVQCKVDA LHSLFHLSTY PPNIPCLLSA DLVNALQSLT
ISDEQRWTEK SLAVLLNLVL NEAGKDEMVS APSLVSNLCT ILDTGEPNEQ EQAVSLLLIL
CNHSEICSEM VLQEGVIPSL VSISVNGTQR GRERAQKLLT LFRELRQRDQ THLTEPQHTE
VTSPEDGFSV ASAAVTETKP QCKSASRKKM GRAFSFLWKS KSFSVYQC
