ID   PUB46_ARATH             Reviewed;         458 AA.
AC   Q3E9F7;
DT   26-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT   08-NOV-2005, sequence version 1.
DT   03-AUG-2022, entry version 102.
DE   RecName: Full=Putative U-box domain-containing protein 46;
DE            EC=2.3.2.27;
DE   AltName: Full=Plant U-box protein 46;
DE   AltName: Full=RING-type E3 ubiquitin transferase PUB46 {ECO:0000305};
GN   Name=PUB46; OrderedLocusNames=At5g18320; ORFNames=F20L16.40;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=11130714; DOI=10.1038/35048507;
RA   Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E.,
RA   Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M.,
RA   Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.,
RA   Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M.,
RA   Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L.,
RA   O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D.,
RA   Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M.,
RA   Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L.,
RA   Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B.,
RA   Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P.,
RA   Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M.,
RA   Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D.,
RA   Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A.,
RA   Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I.,
RA   Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T.,
RA   Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A.,
RA   McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U.,
RA   Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W.,
RA   Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M.,
RA   Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S.,
RA   Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G.,
RA   Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W.,
RA   Bevan M., Fransz P.F.;
RT   "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.";
RL   Nature 408:823-826(2000).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   GENE FAMILY ORGANIZATION.
RX   PubMed=14657406; DOI=10.1104/pp.103.029553;
RA   Mudgil Y., Shiu S.-H., Stone S.L., Salt J.N., Goring D.R.;
RT   "A large complement of the predicted Arabidopsis ARM repeat proteins are
RT   members of the U-box E3 ubiquitin ligase family.";
RL   Plant Physiol. 134:59-66(2004).
CC   -!- FUNCTION: Functions as an E3 ubiquitin ligase. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine +
CC         [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC         cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.;
CC         EC=2.3.2.27;
CC   -!- PATHWAY: Protein modification; protein ubiquitination.
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DR   EMBL; AC051626; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; CP002688; AED92539.1; -; Genomic_DNA.
DR   RefSeq; NP_197333.2; NM_121837.3.
DR   AlphaFoldDB; Q3E9F7; -.
DR   SMR; Q3E9F7; -.
DR   STRING; 3702.AT5G18320.1; -.
DR   iPTMnet; Q3E9F7; -.
DR   PaxDb; Q3E9F7; -.
DR   PRIDE; Q3E9F7; -.
DR   ProteomicsDB; 226110; -.
DR   EnsemblPlants; AT5G18320.1; AT5G18320.1; AT5G18320.
DR   GeneID; 831950; -.
DR   Gramene; AT5G18320.1; AT5G18320.1; AT5G18320.
DR   KEGG; ath:AT5G18320; -.
DR   Araport; AT5G18320; -.
DR   TAIR; locus:2146183; AT5G18320.
DR   eggNOG; KOG0167; Eukaryota.
DR   HOGENOM; CLU_006348_0_0_1; -.
DR   InParanoid; Q3E9F7; -.
DR   OMA; SNTIMIE; -.
DR   OrthoDB; 654013at2759; -.
DR   PhylomeDB; Q3E9F7; -.
DR   UniPathway; UPA00143; -.
DR   PRO; PR:Q3E9F7; -.
DR   Proteomes; UP000006548; Chromosome 5.
DR   ExpressionAtlas; Q3E9F7; baseline and differential.
DR   GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR   GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR   GO; GO:0004842; F:ubiquitin-protein transferase activity; IDA:TAIR.
DR   GO; GO:0042631; P:cellular response to water deprivation; IMP:TAIR.
DR   Gene3D; 1.25.10.10; -; 1.
DR   Gene3D; 3.30.40.10; -; 1.
DR   InterPro; IPR011989; ARM-like.
DR   InterPro; IPR016024; ARM-type_fold.
DR   InterPro; IPR003613; Ubox_domain.
DR   InterPro; IPR013083; Znf_RING/FYVE/PHD.
DR   Pfam; PF04564; U-box; 1.
DR   SMART; SM00504; Ubox; 1.
DR   SUPFAM; SSF48371; SSF48371; 1.
DR   PROSITE; PS51698; U_BOX; 1.
PE   3: Inferred from homology;
KW   Reference proteome; Repeat; Transferase; Ubl conjugation pathway.
FT   CHAIN           1..458
FT                   /note="Putative U-box domain-containing protein 46"
FT                   /id="PRO_0000322186"
FT   DOMAIN          71..144
FT                   /note="U-box"
FT   REPEAT          241..281
FT                   /note="ARM 1"
FT   REPEAT          283..322
FT                   /note="ARM 2"
SQ   SEQUENCE   458 AA;  50872 MW;  17E494415FE43A3C CRC64;
     MADSTETNAD TLRRELQKVL TEILNDGGGN DRDETEAFSG VVKAIDEAVR ILTCLRKVES
     KIPESDISPV EVPKEFICTL SNTIMIEPVI IASGQTYEKR YITEWLKHER TCPKTKQVLS
     HRLWIPNHLI SDLITQWCLV NKYDHQKPSD ELVAELFTSD IEALLQRVSS SSSVADQIEA
     AKELRHQTKK FPNVRVFFVA GIHDSITRLL SPLSTLDEAV DSSLELQENI VTALFNLSIL
     ESNKTVIAEN CLVIPLLTKS LKQGTDETRR NAAATLSSLS AIDSNKIIIG NSEAVKALID
     LIEEGDLLAT KEATSTVFNL CIVLENKGKV VSAGLIHAAT KKIKAGSNVD ELLSLLALIS
     THNRAVEEMD KLGFIYDLFS ILRKPSSLLT GENAVVIVFN MYDRNRDRSR LKVVGEEENQ
     HGTFTKLAKQ GSVRAARKAQ GILQWIKRFV TGKEPQRA