PUB47_ARATH
ID PUB47_ARATH Reviewed; 445 AA.
AC Q3E9F6;
DT 26-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT 08-NOV-2005, sequence version 1.
DT 03-AUG-2022, entry version 107.
DE RecName: Full=Putative U-box domain-containing protein 47;
DE EC=2.3.2.27;
DE AltName: Full=Plant U-box protein 47;
DE AltName: Full=RING-type E3 ubiquitin transferase PUB47 {ECO:0000305};
GN Name=PUB47; OrderedLocusNames=At5g18330; ORFNames=F20L16.50;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130714; DOI=10.1038/35048507;
RA Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E.,
RA Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M.,
RA Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.,
RA Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M.,
RA Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L.,
RA O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D.,
RA Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M.,
RA Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L.,
RA Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B.,
RA Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P.,
RA Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M.,
RA Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D.,
RA Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A.,
RA Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I.,
RA Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T.,
RA Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A.,
RA McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U.,
RA Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W.,
RA Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M.,
RA Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S.,
RA Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G.,
RA Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W.,
RA Bevan M., Fransz P.F.;
RT "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.";
RL Nature 408:823-826(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP GENE FAMILY ORGANIZATION.
RX PubMed=14657406; DOI=10.1104/pp.103.029553;
RA Mudgil Y., Shiu S.-H., Stone S.L., Salt J.N., Goring D.R.;
RT "A large complement of the predicted Arabidopsis ARM repeat proteins are
RT members of the U-box E3 ubiquitin ligase family.";
RL Plant Physiol. 134:59-66(2004).
CC -!- FUNCTION: Functions as an E3 ubiquitin ligase. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine +
CC [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.;
CC EC=2.3.2.27;
CC -!- PATHWAY: Protein modification; protein ubiquitination.
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DR EMBL; AC051626; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CP002688; AED92540.1; -; Genomic_DNA.
DR RefSeq; NP_197334.1; NM_121838.1.
DR AlphaFoldDB; Q3E9F6; -.
DR SMR; Q3E9F6; -.
DR STRING; 3702.AT5G18330.1; -.
DR iPTMnet; Q3E9F6; -.
DR PaxDb; Q3E9F6; -.
DR PRIDE; Q3E9F6; -.
DR ProteomicsDB; 226111; -.
DR EnsemblPlants; AT5G18330.1; AT5G18330.1; AT5G18330.
DR GeneID; 831951; -.
DR Gramene; AT5G18330.1; AT5G18330.1; AT5G18330.
DR KEGG; ath:AT5G18330; -.
DR Araport; AT5G18330; -.
DR TAIR; locus:2146198; AT5G18330.
DR eggNOG; KOG0167; Eukaryota.
DR HOGENOM; CLU_006348_0_0_1; -.
DR InParanoid; Q3E9F6; -.
DR OMA; ERTCPRT; -.
DR OrthoDB; 654013at2759; -.
DR PhylomeDB; Q3E9F6; -.
DR UniPathway; UPA00143; -.
DR PRO; PR:Q3E9F6; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q3E9F6; baseline.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0004842; F:ubiquitin-protein transferase activity; IDA:TAIR.
DR Gene3D; 1.25.10.10; -; 1.
DR Gene3D; 3.30.40.10; -; 1.
DR InterPro; IPR011989; ARM-like.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR003613; Ubox_domain.
DR InterPro; IPR013083; Znf_RING/FYVE/PHD.
DR Pfam; PF04564; U-box; 1.
DR SMART; SM00504; Ubox; 1.
DR SUPFAM; SSF48371; SSF48371; 1.
DR PROSITE; PS51698; U_BOX; 1.
PE 3: Inferred from homology;
KW Reference proteome; Transferase; Ubl conjugation pathway.
FT CHAIN 1..445
FT /note="Putative U-box domain-containing protein 47"
FT /id="PRO_0000322187"
FT DOMAIN 64..137
FT /note="U-box"
SQ SEQUENCE 445 AA; 50089 MW; B9574816A98D7AC7 CRC64;
MADSTADEST NADTLWRELH KVLPEIWYDG GGKDHCEIDE AIRILTCLRK IESKNPESDI
SPVEVPKEFI CTLSNKIMIE PMLIASGQTF EKSYILEWLK HERTCPRTKQ VLYHRFMIPN
HLINEVIKEW CLIHNFDRPK TSDEVIDLFT GDLESLLQRI SSPSSVEDQT EAAKELALKA
KRFSSVCVYF VAKIPDSITR LLTPLSISED SNPEFLENIV TALHIFSTSE KNKTLVAENP
LVLPLLAKYM KQGTVLTRIH SAATVNSLSY TDSNKIIIGN SEVLKALIHV IEEGDSLATS
EAFSALSNLC PVKEISEKAV SEGLIRAAIK KIKAGSNVSM LLSLLAFVST QNHQTTEEMD
NLGLIYDLFS ILRNSNSLVN DENAVVIVYN ICKSYKALQN VVLREEKRDV VLEEENKHGT
FTRLENQEAG RATSLAKRIL EWILR
