PUB48_ARATH
ID PUB48_ARATH Reviewed; 456 AA.
AC Q3E9F5;
DT 26-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT 08-NOV-2005, sequence version 1.
DT 03-AUG-2022, entry version 103.
DE RecName: Full=U-box domain-containing protein 48;
DE EC=2.3.2.27;
DE AltName: Full=Plant U-box protein 48;
DE AltName: Full=RING-type E3 ubiquitin transferase PUB48 {ECO:0000305};
GN Name=PUB48; OrderedLocusNames=At5g18340; ORFNames=F20L16.60;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130714; DOI=10.1038/35048507;
RA Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E.,
RA Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M.,
RA Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.,
RA Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M.,
RA Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L.,
RA O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D.,
RA Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M.,
RA Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L.,
RA Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B.,
RA Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P.,
RA Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M.,
RA Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D.,
RA Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A.,
RA Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I.,
RA Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T.,
RA Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A.,
RA McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U.,
RA Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W.,
RA Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M.,
RA Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S.,
RA Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G.,
RA Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W.,
RA Bevan M., Fransz P.F.;
RT "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.";
RL Nature 408:823-826(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP GENE FAMILY ORGANIZATION.
RX PubMed=14657406; DOI=10.1104/pp.103.029553;
RA Mudgil Y., Shiu S.-H., Stone S.L., Salt J.N., Goring D.R.;
RT "A large complement of the predicted Arabidopsis ARM repeat proteins are
RT members of the U-box E3 ubiquitin ligase family.";
RL Plant Physiol. 134:59-66(2004).
CC -!- FUNCTION: Functions as an E3 ubiquitin ligase. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine +
CC [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.;
CC EC=2.3.2.27;
CC -!- PATHWAY: Protein modification; protein ubiquitination.
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DR EMBL; AC051626; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CP002688; AED92541.1; -; Genomic_DNA.
DR RefSeq; NP_197335.1; NM_121839.3.
DR AlphaFoldDB; Q3E9F5; -.
DR SMR; Q3E9F5; -.
DR PaxDb; Q3E9F5; -.
DR PRIDE; Q3E9F5; -.
DR ProteomicsDB; 226251; -.
DR EnsemblPlants; AT5G18340.1; AT5G18340.1; AT5G18340.
DR GeneID; 831952; -.
DR Gramene; AT5G18340.1; AT5G18340.1; AT5G18340.
DR KEGG; ath:AT5G18340; -.
DR Araport; AT5G18340; -.
DR TAIR; locus:2146213; AT5G18340.
DR HOGENOM; CLU_006348_0_0_1; -.
DR InParanoid; Q3E9F5; -.
DR OMA; ELITEWC; -.
DR OrthoDB; 654013at2759; -.
DR PhylomeDB; Q3E9F5; -.
DR UniPathway; UPA00143; -.
DR PRO; PR:Q3E9F5; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q3E9F5; baseline and differential.
DR Genevisible; Q3E9F5; AT.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0061630; F:ubiquitin protein ligase activity; IDA:TAIR.
DR GO; GO:0004842; F:ubiquitin-protein transferase activity; IDA:TAIR.
DR GO; GO:1900034; P:regulation of cellular response to heat; IMP:TAIR.
DR Gene3D; 1.25.10.10; -; 1.
DR Gene3D; 3.30.40.10; -; 1.
DR InterPro; IPR011989; ARM-like.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR003613; Ubox_domain.
DR InterPro; IPR013083; Znf_RING/FYVE/PHD.
DR Pfam; PF04564; U-box; 1.
DR SMART; SM00504; Ubox; 1.
DR SUPFAM; SSF48371; SSF48371; 1.
DR PROSITE; PS51698; U_BOX; 1.
PE 2: Evidence at transcript level;
KW Reference proteome; Repeat; Transferase; Ubl conjugation pathway.
FT CHAIN 1..456
FT /note="U-box domain-containing protein 48"
FT /id="PRO_0000322188"
FT DOMAIN 73..146
FT /note="U-box"
FT REPEAT 247..287
FT /note="ARM 1"
FT REPEAT 289..329
FT /note="ARM 2"
SQ SEQUENCE 456 AA; 50792 MW; E727D403718BCAF3 CRC64;
MADSTADATE TNADTLTLRR ELKKVLTENL NDGGVKDRVE TVKSIDEAIR ILNRLKIVES
KKRKRESDSS SVEVPKEFKC TLSKTIMIDP VIIFSGQTYE KRYITEWLNH DLTCPTAKQV
LYRVCLTPNH LINELITRWC LANKYDRPAP KPSDIDYVTE LFTDGIESLL QRISSPSSSV
ADQTEAAKEL ALQTEKFVNV RDFFIKELPD SITRLLTPLS VLGDEVDSNP ELQENIVTAL
FNMSTFEKNK TVLAENHQVI PLLAKSMKQG SVVTRRNATL TLASLSDIDS NKIIIGNSVA
LKALIDLIGE LDDLSATHDA LCAVIDLCCD ERENWKKAIS LGLAPAAIKN IKARRNLFES
LAALALISPH ERVIQEVANL GVIYDLLSIL RKTSCMVTCE NAVVIVGNMY AKSRERSIKK
ILAEEENQHK TFTKIATQGS VVAVMKAQGI LQCINY
