PUB4_ARATH
ID PUB4_ARATH Reviewed; 826 AA.
AC O22193; F4ILG7;
DT 15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT 16-NOV-2011, sequence version 3.
DT 25-MAY-2022, entry version 146.
DE RecName: Full=U-box domain-containing protein 4;
DE EC=2.3.2.27;
DE AltName: Full=Plant U-box protein 4;
DE AltName: Full=RING-type E3 ubiquitin transferase PUB4 {ECO:0000305};
GN Name=PUB4; OrderedLocusNames=At2g23140; ORFNames=T20D16.23;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP GENE FAMILY ORGANIZATION, AND NOMENCLATURE.
RX PubMed=11495788; DOI=10.1016/s1360-1385(01)01960-4;
RA Azevedo C., Santos-Rosa M.J., Shirasu K.;
RT "The U-box protein family in plants.";
RL Trends Plant Sci. 6:354-358(2001).
RN [4]
RP GENE FAMILY ORGANIZATION.
RX PubMed=14657406; DOI=10.1104/pp.103.029553;
RA Mudgil Y., Shiu S.-H., Stone S.L., Salt J.N., Goring D.R.;
RT "A large complement of the predicted Arabidopsis ARM repeat proteins are
RT members of the U-box E3 ubiquitin ligase family.";
RL Plant Physiol. 134:59-66(2004).
RN [5]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-396, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19376835; DOI=10.1104/pp.109.138677;
RA Reiland S., Messerli G., Baerenfaller K., Gerrits B., Endler A.,
RA Grossmann J., Gruissem W., Baginsky S.;
RT "Large-scale Arabidopsis phosphoproteome profiling reveals novel
RT chloroplast kinase substrates and phosphorylation networks.";
RL Plant Physiol. 150:889-903(2009).
CC -!- FUNCTION: Functions as an E3 ubiquitin ligase. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine +
CC [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.;
CC EC=2.3.2.27;
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=1;
CC Comment=A number of isoforms are produced. According to EST
CC sequences.;
CC Name=1;
CC IsoId=O22193-1; Sequence=Displayed;
CC -!- SEQUENCE CAUTION:
CC Sequence=AAB87116.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=AAM14930.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC002391; AAB87116.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AC004401; AAM14930.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002685; AEC07418.1; -; Genomic_DNA.
DR EMBL; CP002685; ANM61792.1; -; Genomic_DNA.
DR PIR; T00518; T00518.
DR RefSeq; NP_001189583.1; NM_001202654.2. [O22193-1]
DR RefSeq; NP_001323989.1; NM_001335839.1. [O22193-1]
DR AlphaFoldDB; O22193; -.
DR SMR; O22193; -.
DR STRING; 3702.AT2G23140.1; -.
DR iPTMnet; O22193; -.
DR PaxDb; O22193; -.
DR PRIDE; O22193; -.
DR ProteomicsDB; 226252; -. [O22193-1]
DR EnsemblPlants; AT2G23140.2; AT2G23140.2; AT2G23140. [O22193-1]
DR EnsemblPlants; AT2G23140.4; AT2G23140.4; AT2G23140. [O22193-1]
DR GeneID; 816846; -.
DR Gramene; AT2G23140.2; AT2G23140.2; AT2G23140. [O22193-1]
DR Gramene; AT2G23140.4; AT2G23140.4; AT2G23140. [O22193-1]
DR KEGG; ath:AT2G23140; -.
DR Araport; AT2G23140; -.
DR eggNOG; KOG0167; Eukaryota.
DR HOGENOM; CLU_006348_5_0_1; -.
DR InParanoid; O22193; -.
DR UniPathway; UPA00143; -.
DR PRO; PR:O22193; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; O22193; baseline and differential.
DR Genevisible; O22193; AT.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0004842; F:ubiquitin-protein transferase activity; IEA:InterPro.
DR CDD; cd16664; RING-Ubox_PUB; 1.
DR Gene3D; 1.25.10.10; -; 1.
DR Gene3D; 3.30.40.10; -; 1.
DR InterPro; IPR011989; ARM-like.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR000225; Armadillo.
DR InterPro; IPR045210; RING-Ubox_PUB.
DR InterPro; IPR003613; Ubox_domain.
DR InterPro; IPR013083; Znf_RING/FYVE/PHD.
DR Pfam; PF00514; Arm; 4.
DR Pfam; PF04564; U-box; 1.
DR SMART; SM00185; ARM; 5.
DR SMART; SM00504; Ubox; 1.
DR SUPFAM; SSF48371; SSF48371; 1.
DR PROSITE; PS50176; ARM_REPEAT; 4.
DR PROSITE; PS51698; U_BOX; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Coiled coil; Phosphoprotein; Reference proteome;
KW Repeat; Transferase; Ubl conjugation pathway.
FT CHAIN 1..826
FT /note="U-box domain-containing protein 4"
FT /id="PRO_0000315407"
FT DOMAIN 229..303
FT /note="U-box"
FT REPEAT 530..570
FT /note="ARM 1"
FT REPEAT 573..612
FT /note="ARM 2"
FT REPEAT 614..653
FT /note="ARM 3"
FT REPEAT 655..694
FT /note="ARM 4"
FT REPEAT 696..734
FT /note="ARM 5"
FT REPEAT 736..775
FT /note="ARM 6"
FT REPEAT 778..817
FT /note="ARM 7"
FT REGION 330..501
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 172..204
FT /evidence="ECO:0000255"
FT COMPBIAS 349..365
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 395..413
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 414..455
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 463..495
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 396
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:19376835"
SQ SEQUENCE 826 AA; 89986 MW; 5365B77FDC6FC8BB CRC64;
MEVLLRSISS FLNLSSSKHI DLDPFEKYYK RVEELLRVLK PIADVVVTSD FVFDEKLGKA
FEELTQDVDQ SIDLFRSWQA FSSKVYFVLQ IESLLPKMRD TIVDTFQFLM SSKNHLPDEL
SPASLEQCLE KIKHLSYEEI SSVIDGALRD QRDGVGPSPE ILVKIGENTG LRSNQEILIE
AVALERQKEM AEQSENNAEV EFLDQLIVIV NRMHERLLLI KQTQTSSVAI LADFFCPLSL
EVMTDPVIVS SGQTYEKAFI KRWIDLGLKV CPKTRQTLTH TTLIPNYTVK ALIANWCETN
DVKLPDPNKS TSLNELSPLL SCTDSIPSTG ADVSARKVSN KSHDWDASSS ETGKPSFSSR
ATEREGASPS RPASALGASS PGISGNGYGL DARRGSLNDF EDRSNDSREL RTDAPGRSSV
SSTTRGSVEN GQTSENHHHR SPSATSTVSN EEFPRADANE NSEESAHATP YSSDASGEIR
SGPLAATTSA ATRRDLSDFS PKFMDRRTRG QFWRRPSERL GSRIVSAPSN ETRRDLSEVE
TQVKKLVEEL KSSSLDTQRQ ATAELRLLAK HNMDNRIVIG NSGAIVLLVE LLYSTDSATQ
ENAVTALLNL SINDNNKKAI ADAGAIEPLI HVLENGSSEA KENSAATLFS LSVIEENKIK
IGQSGAIGPL VDLLGNGTPR GKKDAATALF NLSIHQENKA MIVQSGAVRY LIDLMDPAAG
MVDKAVAVLA NLATIPEGRN AIGQEGGIPL LVEVVELGSA RGKENAAAAL LQLSTNSGRF
CNMVLQEGAV PPLVALSQSG TPRAREKAQA LLSYFRNQRH GNAGRG
