PUB54_ARATH
ID PUB54_ARATH Reviewed; 308 AA.
AC Q9LQ92;
DT 26-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 116.
DE RecName: Full=U-box domain-containing protein 54;
DE EC=2.3.2.27;
DE AltName: Full=Plant U-box protein 54;
DE AltName: Full=RING-type E3 ubiquitin transferase PUB54 {ECO:0000305};
GN Name=PUB54; OrderedLocusNames=At1g01680; ORFNames=T1N6.5;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
CC -!- FUNCTION: Functions as an E3 ubiquitin ligase. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine +
CC [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.;
CC EC=2.3.2.27;
CC -!- PATHWAY: Protein modification; protein ubiquitination.
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DR EMBL; AC009273; AAF78398.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE27321.1; -; Genomic_DNA.
DR PIR; F86147; F86147.
DR RefSeq; NP_171674.2; NM_100050.4.
DR AlphaFoldDB; Q9LQ92; -.
DR SMR; Q9LQ92; -.
DR STRING; 3702.AT1G01680.1; -.
DR PaxDb; Q9LQ92; -.
DR PRIDE; Q9LQ92; -.
DR ProteomicsDB; 226112; -.
DR EnsemblPlants; AT1G01680.1; AT1G01680.1; AT1G01680.
DR GeneID; 839251; -.
DR Gramene; AT1G01680.1; AT1G01680.1; AT1G01680.
DR KEGG; ath:AT1G01680; -.
DR Araport; AT1G01680; -.
DR TAIR; locus:2198190; AT1G01680.
DR HOGENOM; CLU_1066901_0_0_1; -.
DR InParanoid; Q9LQ92; -.
DR OMA; LEDFKCP; -.
DR OrthoDB; 957291at2759; -.
DR PhylomeDB; Q9LQ92; -.
DR BRENDA; 2.3.2.27; 399.
DR UniPathway; UPA00143; -.
DR PRO; PR:Q9LQ92; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9LQ92; baseline and differential.
DR GO; GO:0004842; F:ubiquitin-protein transferase activity; IEA:InterPro.
DR Gene3D; 3.30.40.10; -; 1.
DR Gene3D; 3.40.50.620; -; 1.
DR InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR InterPro; IPR003613; Ubox_domain.
DR InterPro; IPR006016; UspA.
DR InterPro; IPR013083; Znf_RING/FYVE/PHD.
DR Pfam; PF04564; U-box; 1.
DR Pfam; PF00582; Usp; 1.
DR SMART; SM00504; Ubox; 1.
DR PROSITE; PS51698; U_BOX; 1.
PE 2: Evidence at transcript level;
KW Reference proteome; Transferase; Ubl conjugation pathway.
FT CHAIN 1..308
FT /note="U-box domain-containing protein 54"
FT /id="PRO_0000322189"
FT DOMAIN 232..306
FT /note="U-box"
FT REGION 172..235
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 181..235
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 308 AA; 35392 MW; CE53CFA27C77547D CRC64;
MEDAIYVAVN QDVRESKKTL LWALKNLQVK KIFLLHVHLP FSLTTSSSRL EQSEIDAIQD
SELNTSVNSL YKYRDICINK GVNEKDVDTS MISGHDVGEG IVELIYQNII TNLVMGAAAD
PHYSRGMSIT SRKAEYVSQH APHSCKIWFI CKGKLIKQRE RSFYLGNPSD SFSEFSTSAE
KPISKGRRRD EEEEPESPKE HPGWILEPEE SPKKGRKETI EKSKSNESDE DPRLEDFKCP
ISMEIMRDPH VAADGFTYEA EEFRKWLRSG GRTSPKTNKP LENHNLVPNH TLRIIIKDWL
EKNPNYKR
