ID   PUB56_ARATH             Reviewed;         365 AA.
AC   Q8GXQ7; Q9LQ93;
DT   26-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2003, sequence version 1.
DT   03-AUG-2022, entry version 95.
DE   RecName: Full=U-box domain-containing protein 56;
DE            EC=2.3.2.27;
DE   AltName: Full=Plant U-box protein 56;
DE   AltName: Full=RING-type E3 ubiquitin transferase PUB56 {ECO:0000305};
GN   Name=PUB56; OrderedLocusNames=At1g01670; ORFNames=T1N6.28;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=11130712; DOI=10.1038/35048500;
RA   Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA   Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA   Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA   Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA   Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA   Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA   Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA   Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA   Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA   Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA   Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA   Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA   Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA   Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA   Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT   "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL   Nature 408:816-820(2000).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=11910074; DOI=10.1126/science.1071006;
RA   Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T.,
RA   Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y.,
RA   Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K.,
RA   Shinagawa A., Shinozaki K.;
RT   "Functional annotation of a full-length Arabidopsis cDNA collection.";
RL   Science 296:141-145(2002).
CC   -!- FUNCTION: Functions as an E3 ubiquitin ligase. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine +
CC         [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC         cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.;
CC         EC=2.3.2.27;
CC   -!- PATHWAY: Protein modification; protein ubiquitination.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAF78397.1; Type=Erroneous gene model prediction; Note=The predicted gene has been split into 2 genes: At1g01660 and At1g01670.; Evidence={ECO:0000305};
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DR   EMBL; AC009273; AAF78397.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; CP002684; AEE27320.1; -; Genomic_DNA.
DR   EMBL; CP002684; ANM59608.1; -; Genomic_DNA.
DR   EMBL; AK118101; BAC42729.1; -; mRNA.
DR   PIR; E86147; E86147.
DR   RefSeq; NP_001321953.1; NM_001331289.1.
DR   RefSeq; NP_171673.2; NM_100049.4.
DR   AlphaFoldDB; Q8GXQ7; -.
DR   SMR; Q8GXQ7; -.
DR   PaxDb; Q8GXQ7; -.
DR   PRIDE; Q8GXQ7; -.
DR   EnsemblPlants; AT1G01670.1; AT1G01670.1; AT1G01670.
DR   EnsemblPlants; AT1G01670.2; AT1G01670.2; AT1G01670.
DR   GeneID; 839365; -.
DR   Gramene; AT1G01670.1; AT1G01670.1; AT1G01670.
DR   Gramene; AT1G01670.2; AT1G01670.2; AT1G01670.
DR   KEGG; ath:AT1G01670; -.
DR   Araport; AT1G01670; -.
DR   TAIR; locus:2198180; AT1G01670.
DR   eggNOG; ENOG502R7RK; Eukaryota.
DR   HOGENOM; CLU_036548_0_0_1; -.
DR   InParanoid; Q8GXQ7; -.
DR   OMA; CKIWLVC; -.
DR   OrthoDB; 957291at2759; -.
DR   PhylomeDB; Q8GXQ7; -.
DR   UniPathway; UPA00143; -.
DR   PRO; PR:Q8GXQ7; -.
DR   Proteomes; UP000006548; Chromosome 1.
DR   ExpressionAtlas; Q8GXQ7; baseline and differential.
DR   Genevisible; Q8GXQ7; AT.
DR   GO; GO:0004842; F:ubiquitin-protein transferase activity; IEA:InterPro.
DR   Gene3D; 3.30.40.10; -; 1.
DR   InterPro; IPR003613; Ubox_domain.
DR   InterPro; IPR013083; Znf_RING/FYVE/PHD.
DR   Pfam; PF04564; U-box; 1.
DR   SMART; SM00504; Ubox; 1.
DR   PROSITE; PS51698; U_BOX; 1.
PE   2: Evidence at transcript level;
KW   Coiled coil; Reference proteome; Transferase; Ubl conjugation pathway.
FT   CHAIN           1..365
FT                   /note="U-box domain-containing protein 56"
FT                   /id="PRO_0000322191"
FT   DOMAIN          293..365
FT                   /note="U-box"
FT   COILED          176..281
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   365 AA;  41864 MW;  9AC4C736173DD0D4 CRC64;
     MTPSSSGLEQ SEIDAIQELE QTSRNDTLLK YHDICIDEGV IEQDVDMSCF SANSVGEWIV
     ELIYQNNIKK LIMGATADSH YSEGMVHITP TKADYVIQHA PHCCNIWLVC NGNLIQTREG
     RFEHAGSAYS SSSSLHSIDS ALIPYGGAGR AERVTEPHAL SSSEEQSARG IEKMYYEEQR
     RRLEIEELKR EKEQRDKMRR VREEALSSSS GVTKILYNEE VMRRREVEAE LNRAKAEIED
     MKRVQIELKE QHYADCRLLE KERDEAIKTT EELLRALEKG ESSIPLQWSV SIEPPQCFIC
     PISKDIMQNP HVAADGYTYE ADEFRRWLNH GGEKSPMTNL RLENRNLIPN LVLRSAIKDW
     LQQHP