PUB57_ARATH
ID PUB57_ARATH Reviewed; 391 AA.
AC Q9SGT1; F4I3I7; Q1PFJ9; Q9LG06;
DT 26-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT 26-FEB-2008, sequence version 2.
DT 03-AUG-2022, entry version 97.
DE RecName: Full=U-box domain-containing protein 57;
DE EC=2.3.2.27;
DE AltName: Full=Plant U-box protein 57;
DE AltName: Full=RING-type E3 ubiquitin transferase PUB57 {ECO:0000305};
GN Name=PUB57; OrderedLocusNames=At1g56030; ORFNames=F14J16.34, T6H22.17;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=cv. Columbia;
RX PubMed=17147637; DOI=10.1111/j.1467-7652.2006.00183.x;
RA Underwood B.A., Vanderhaeghen R., Whitford R., Town C.D., Hilson P.;
RT "Simultaneous high-throughput recombinational cloning of open reading
RT frames in closed and open configurations.";
RL Plant Biotechnol. J. 4:317-324(2006).
CC -!- FUNCTION: Functions as an E3 ubiquitin ligase. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine +
CC [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.;
CC EC=2.3.2.27;
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q9SGT1-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9SGT1-2; Sequence=VSP_031885;
CC -!- MISCELLANEOUS: [Isoform 2]: May be due to intron retention.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAF02851.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=AAF79327.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC002304; AAF79327.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AC009894; AAF02851.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE33333.2; -; Genomic_DNA.
DR EMBL; DQ446364; ABE65715.1; -; mRNA.
DR PIR; E96601; E96601.
DR RefSeq; NP_001319249.1; NM_001333757.1. [Q9SGT1-1]
DR AlphaFoldDB; Q9SGT1; -.
DR SMR; Q9SGT1; -.
DR PRIDE; Q9SGT1; -.
DR ProteomicsDB; 224856; -. [Q9SGT1-1]
DR EnsemblPlants; AT1G56030.1; AT1G56030.1; AT1G56030. [Q9SGT1-1]
DR GeneID; 842054; -.
DR Gramene; AT1G56030.1; AT1G56030.1; AT1G56030. [Q9SGT1-1]
DR KEGG; ath:AT1G56030; -.
DR Araport; AT1G56030; -.
DR eggNOG; ENOG502S95A; Eukaryota.
DR HOGENOM; CLU_626068_0_0_1; -.
DR InParanoid; Q9SGT1; -.
DR OrthoDB; 957291at2759; -.
DR PhylomeDB; Q9SGT1; -.
DR UniPathway; UPA00143; -.
DR PRO; PR:Q9SGT1; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9SGT1; baseline and differential.
DR Genevisible; Q9SGT1; AT.
DR GO; GO:0004842; F:ubiquitin-protein transferase activity; IEA:InterPro.
DR Gene3D; 3.30.40.10; -; 1.
DR InterPro; IPR003613; Ubox_domain.
DR InterPro; IPR013083; Znf_RING/FYVE/PHD.
DR Pfam; PF04564; U-box; 1.
DR SMART; SM00504; Ubox; 1.
DR PROSITE; PS51698; U_BOX; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Reference proteome; Transferase;
KW Ubl conjugation pathway.
FT CHAIN 1..391
FT /note="U-box domain-containing protein 57"
FT /id="PRO_0000322192"
FT DOMAIN 1..178
FT /note="MIF4G"
FT DOMAIN 322..391
FT /note="U-box"
FT VAR_SEQ 1..204
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:17147637"
FT /id="VSP_031885"
SQ SEQUENCE 391 AA; 45583 MW; 0C9379C4F7E2A199 CRC64;
MVKNSYVLFA RLCVELPPLP SDESHGEITT VERQFLRNCQ MELENLSLKT ELPLVYVDES
KYWRFIKTVR VLAEVFNNMK TTRTTRKSII QVLMNPILPS ERSTDAMNLF LSTIEKLADL
QFSDEDFNQL FVSSRLDLQL ENKYNDKVEV KLRKEAEDAL ARKIKEVVDL TERLLQVEAL
EHKHKAKLQL RTETETAVAI ERDYMRWKAE IFESEFNNQL VLRRESEIAL DKERKELEGI
KNLLETCFTG QKNLKSQVIT WKDKYDQGSS IRKEKEVALS TKKLELEIFK QLAGSYKQDA
DAMRQERDNA LKTVQEIVDE QQPPPSFICP ITQDVMKNPH MAADGFTYEL EAIQKWINTG
HRTSPMTNLK LSHFSFFPNR ALRSAIEELG R
