ID   PUB58_ARATH             Reviewed;         420 AA.
AC   Q9SGT2; F4I3I8;
DT   26-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT   31-MAY-2011, sequence version 3.
DT   03-AUG-2022, entry version 97.
DE   RecName: Full=Putative U-box domain-containing protein 58;
DE            EC=2.3.2.27;
DE   AltName: Full=Plant U-box protein 58;
DE   AltName: Full=RING-type E3 ubiquitin transferase PUB58 {ECO:0000305};
GN   Name=PUB58; OrderedLocusNames=At1g56040; ORFNames=T6H22.16;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=11130712; DOI=10.1038/35048500;
RA   Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA   Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA   Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA   Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA   Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA   Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA   Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA   Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA   Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA   Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA   Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA   Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA   Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA   Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA   Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT   "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL   Nature 408:816-820(2000).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
CC   -!- FUNCTION: Functions as an E3 ubiquitin ligase. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine +
CC         [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC         cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.;
CC         EC=2.3.2.27;
CC   -!- PATHWAY: Protein modification; protein ubiquitination.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAF02844.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR   EMBL; AC009894; AAF02844.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; CP002684; AEE33334.2; -; Genomic_DNA.
DR   PIR; F96601; F96601.
DR   RefSeq; NP_176000.5; NM_104482.5.
DR   AlphaFoldDB; Q9SGT2; -.
DR   SMR; Q9SGT2; -.
DR   BioGRID; 27280; 1.
DR   STRING; 3702.AT1G56040.1; -.
DR   PaxDb; Q9SGT2; -.
DR   PRIDE; Q9SGT2; -.
DR   ProteomicsDB; 226255; -.
DR   EnsemblPlants; AT1G56040.1; AT1G56040.1; AT1G56040.
DR   GeneID; 842055; -.
DR   Gramene; AT1G56040.1; AT1G56040.1; AT1G56040.
DR   KEGG; ath:AT1G56040; -.
DR   Araport; AT1G56040; -.
DR   eggNOG; ENOG502S95A; Eukaryota.
DR   HOGENOM; CLU_626068_0_0_1; -.
DR   InParanoid; Q9SGT2; -.
DR   OMA; RKCRIEL; -.
DR   OrthoDB; 596957at2759; -.
DR   UniPathway; UPA00143; -.
DR   PRO; PR:Q9SGT2; -.
DR   Proteomes; UP000006548; Chromosome 1.
DR   ExpressionAtlas; Q9SGT2; baseline and differential.
DR   Genevisible; Q9SGT2; AT.
DR   GO; GO:0004842; F:ubiquitin-protein transferase activity; IEA:InterPro.
DR   Gene3D; 3.30.40.10; -; 1.
DR   InterPro; IPR016024; ARM-type_fold.
DR   InterPro; IPR003613; Ubox_domain.
DR   InterPro; IPR013083; Znf_RING/FYVE/PHD.
DR   Pfam; PF04564; U-box; 1.
DR   SMART; SM00504; Ubox; 1.
DR   SUPFAM; SSF48371; SSF48371; 1.
DR   PROSITE; PS51698; U_BOX; 1.
PE   3: Inferred from homology;
KW   Coiled coil; Reference proteome; Transferase; Ubl conjugation pathway.
FT   CHAIN           1..420
FT                   /note="Putative U-box domain-containing protein 58"
FT                   /id="PRO_0000322193"
FT   DOMAIN          4..168
FT                   /note="MIF4G"
FT   DOMAIN          352..420
FT                   /note="U-box"
FT   COILED          139..352
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   420 AA;  49531 MW;  4E0A3098A5F3A03D CRC64;
     MVENSYVLFA RLCVELFHEL PPLPSDESHG DITTFERLFL RKCRIELENA SPKTEHLPLV
     YVDETNYYRF IKTVRVLAEV YKNSKITETT RKSMIQVLMN PILPPERITD AMNLFRSIIG
     KLADFHFSDE KFNQLVRSSR VVELEGNYNE EVKLRKEAED ALAMKKEDVE MMEQLLESYK
     EEQGKLQLQA KALEHKLEAE LRHRKETETL LAIERDRIEK VKIQLETVEN EIDNTRLKAE
     EFERKYEGEM ILRRESEIAL EKEKKELEEV KLKLETYERE QENLSSEVRT WQDKYEQESS
     LRKLSEYALS REQEELQIVK GLLEFYNGEA DAMREERDKA LKTAKEQMEK RQPPSSFFCP
     ITQEVMKDPH FAADGFTYEA ESIRKWLSTG HQTSPMTNLR LSHLTLVPNR ALRSAIEELV