PUB63_ARATH
ID PUB63_ARATH Reviewed; 383 AA.
AC Q58FY4; O22871; Q0WUK1; Q67XS7; Q680U6; Q680Z3; Q8GUT1;
DT 26-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT 26-APR-2005, sequence version 1.
DT 03-AUG-2022, entry version 103.
DE RecName: Full=U-box domain-containing protein 63;
DE EC=2.3.2.27;
DE AltName: Full=Plant U-box protein 63;
DE AltName: Full=RING-type E3 ubiquitin transferase PUB63 {ECO:0000305};
GN Name=PUB63; OrderedLocusNames=At2g40640; ORFNames=T2P4.1, T7D17.18;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
RC STRAIN=cv. Columbia;
RX PubMed=14993207; DOI=10.1101/gr.1515604;
RA Castelli V., Aury J.-M., Jaillon O., Wincker P., Clepet C., Menard M.,
RA Cruaud C., Quetier F., Scarpelli C., Schaechter V., Temple G., Caboche M.,
RA Weissenbach J., Salanoubat M.;
RT "Whole genome sequence comparisons and 'full-length' cDNA sequences: a
RT combined approach to evaluate and improve Arabidopsis genome annotation.";
RL Genome Res. 14:406-413(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RX PubMed=16244158; DOI=10.1104/pp.105.063479;
RA Xiao Y.-L., Smith S.R., Ishmael N., Redman J.C., Kumar N., Monaghan E.L.,
RA Ayele M., Haas B.J., Wu H.C., Town C.D.;
RT "Analysis of the cDNAs of hypothetical genes on Arabidopsis chromosome 2
RT reveals numerous transcript variants.";
RL Plant Physiol. 139:1323-1337(2005).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RA Underwood B.A., Xiao Y.-L., Moskal W.A. Jr., Monaghan E.L., Wang W.,
RA Redman J.C., Wu H.C., Utterback T., Town C.D.;
RL Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 2-383 (ISOFORMS 1 AND 2).
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Functions as an E3 ubiquitin ligase. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine +
CC [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.;
CC EC=2.3.2.27;
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=Q58FY4-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q58FY4-2; Sequence=VSP_031886;
CC Name=3;
CC IsoId=Q58FY4-3; Sequence=VSP_038053;
CC -!- MISCELLANEOUS: [Isoform 2]: May be due to a competing donor splice
CC site. {ECO:0000305}.
CC -!- MISCELLANEOUS: [Isoform 3]: Incomplete sequence. May be due to an
CC intron retention. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAM14813.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=BAE99197.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AC002336; AAM14813.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002685; AEC09857.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC09858.1; -; Genomic_DNA.
DR EMBL; AY168999; AAO11670.1; -; mRNA.
DR EMBL; AY954825; AAX55151.1; -; mRNA.
DR EMBL; AK175771; BAD43534.1; -; mRNA.
DR EMBL; AK176741; BAD44504.1; -; mRNA.
DR EMBL; AK227154; BAE99197.1; ALT_INIT; mRNA.
DR EMBL; AK175678; BAD43441.1; -; mRNA.
DR EMBL; AK175724; BAD43487.1; -; mRNA.
DR PIR; A84832; A84832.
DR RefSeq; NP_850332.1; NM_180001.4. [Q58FY4-1]
DR RefSeq; NP_850333.1; NM_180002.3. [Q58FY4-3]
DR AlphaFoldDB; Q58FY4; -.
DR iPTMnet; Q58FY4; -.
DR PaxDb; Q58FY4; -.
DR PRIDE; Q58FY4; -.
DR ProteomicsDB; 224860; -. [Q58FY4-1]
DR EnsemblPlants; AT2G40640.1; AT2G40640.1; AT2G40640. [Q58FY4-1]
DR EnsemblPlants; AT2G40640.2; AT2G40640.2; AT2G40640. [Q58FY4-3]
DR GeneID; 818659; -.
DR Gramene; AT2G40640.1; AT2G40640.1; AT2G40640. [Q58FY4-1]
DR Gramene; AT2G40640.2; AT2G40640.2; AT2G40640. [Q58FY4-3]
DR KEGG; ath:AT2G40640; -.
DR Araport; AT2G40640; -.
DR TAIR; locus:2061823; AT2G40640.
DR eggNOG; ENOG502QT2D; Eukaryota.
DR HOGENOM; CLU_788338_0_0_1; -.
DR InParanoid; Q58FY4; -.
DR OrthoDB; 983154at2759; -.
DR PhylomeDB; Q58FY4; -.
DR UniPathway; UPA00143; -.
DR PRO; PR:Q58FY4; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q58FY4; baseline and differential.
DR Genevisible; Q58FY4; AT.
DR GO; GO:0004842; F:ubiquitin-protein transferase activity; IEA:InterPro.
DR Gene3D; 3.30.40.10; -; 1.
DR InterPro; IPR003613; Ubox_domain.
DR InterPro; IPR013083; Znf_RING/FYVE/PHD.
DR PROSITE; PS51698; U_BOX; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Reference proteome; Transferase;
KW Ubl conjugation pathway.
FT CHAIN 1..383
FT /note="U-box domain-containing protein 63"
FT /id="PRO_0000322195"
FT DOMAIN 201..273
FT /note="U-box"
FT REGION 166..186
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 285..383
FT /note="SDQKRSFCIPNITETPKSSRGIQFPFSIGDHIIIEGNKRTPPRFVGRIAVIM
FT TQCLNGWYVVKTVDNSESIKLQHCSLAKISDNSSTKVTVAEMPPSWL -> REVSVSQT
FT SLKLLRVAEAYSFLSPLEIILS (in isoform 2)"
FT /evidence="ECO:0000303|Ref.6"
FT /id="VSP_031886"
FT VAR_SEQ 344..383
FT /note="YVVKTVDNSESIKLQHCSLAKISDNSSTKVTVAEMPPSWL -> LSLVASFH
FT P (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:14993207"
FT /id="VSP_038053"
SQ SEQUENCE 383 AA; 43302 MW; 8B0D35410EAA1C86 CRC64;
MSHLRRRRFK LSLCALPVSI DRLLNFPAEK QMSVNDSIDP RFVFHVEDES LRFRVGDSGT
KIRELETVGD RHFRTQQGFC GNRIEKDETM YSDGDDEEYD VSIRRRTALI QPGIDSGNNY
DSAAAEETNR ESKNPVGWEL VVREDGEGNS TIDRHEMEFK VRITKPDGNV SNSHRNTQQK
RDFASVEKER VTTTSVSSWE SLKAILSDPV TGALMNDATI LPCGHSFGAG GLKEVKKMKA
CFTCSQPTLE GSEKPNLSLR IVVHAFRQEE DSDHIHTLKR RKERSDQKRS FCIPNITETP
KSSRGIQFPF SIGDHIIIEG NKRTPPRFVG RIAVIMTQCL NGWYVVKTVD NSESIKLQHC
SLAKISDNSS TKVTVAEMPP SWL
