PUB6_ARATH
ID PUB6_ARATH Reviewed; 771 AA.
AC O48700;
DT 26-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT 26-FEB-2008, sequence version 2.
DT 03-AUG-2022, entry version 140.
DE RecName: Full=U-box domain-containing protein 6;
DE EC=2.3.2.27;
DE AltName: Full=Plant U-box protein 6;
DE AltName: Full=RING-type E3 ubiquitin transferase PUB6 {ECO:0000305};
GN Name=PUB6; OrderedLocusNames=At1g24330; ORFNames=F3I6.27;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP GENE FAMILY ORGANIZATION, AND NOMENCLATURE.
RX PubMed=11495788; DOI=10.1016/s1360-1385(01)01960-4;
RA Azevedo C., Santos-Rosa M.J., Shirasu K.;
RT "The U-box protein family in plants.";
RL Trends Plant Sci. 6:354-358(2001).
RN [4]
RP GENE FAMILY ORGANIZATION.
RX PubMed=14657406; DOI=10.1104/pp.103.029553;
RA Mudgil Y., Shiu S.-H., Stone S.L., Salt J.N., Goring D.R.;
RT "A large complement of the predicted Arabidopsis ARM repeat proteins are
RT members of the U-box E3 ubiquitin ligase family.";
RL Plant Physiol. 134:59-66(2004).
CC -!- FUNCTION: Functions as an E3 ubiquitin ligase. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine +
CC [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.;
CC EC=2.3.2.27;
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAC00595.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC002396; AAC00595.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE30517.1; -; Genomic_DNA.
DR EMBL; CP002684; ANM61155.1; -; Genomic_DNA.
DR PIR; T00664; T00664.
DR RefSeq; NP_001323390.1; NM_001332646.1.
DR RefSeq; NP_173843.2; NM_102279.4.
DR AlphaFoldDB; O48700; -.
DR SMR; O48700; -.
DR iPTMnet; O48700; -.
DR PaxDb; O48700; -.
DR PRIDE; O48700; -.
DR ProteomicsDB; 226258; -.
DR EnsemblPlants; AT1G24330.1; AT1G24330.1; AT1G24330.
DR EnsemblPlants; AT1G24330.2; AT1G24330.2; AT1G24330.
DR GeneID; 839049; -.
DR Gramene; AT1G24330.1; AT1G24330.1; AT1G24330.
DR Gramene; AT1G24330.2; AT1G24330.2; AT1G24330.
DR KEGG; ath:AT1G24330; -.
DR Araport; AT1G24330; -.
DR TAIR; locus:2032472; AT1G24330.
DR eggNOG; KOG0167; Eukaryota.
DR HOGENOM; CLU_006348_4_1_1; -.
DR InParanoid; O48700; -.
DR OMA; LERMISC; -.
DR OrthoDB; 174560at2759; -.
DR PhylomeDB; O48700; -.
DR UniPathway; UPA00143; -.
DR PRO; PR:O48700; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; O48700; baseline and differential.
DR Genevisible; O48700; AT.
DR GO; GO:0009507; C:chloroplast; HDA:TAIR.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0004842; F:ubiquitin-protein transferase activity; IEA:InterPro.
DR CDD; cd16664; RING-Ubox_PUB; 1.
DR Gene3D; 1.25.10.10; -; 1.
DR Gene3D; 3.30.40.10; -; 1.
DR InterPro; IPR011989; ARM-like.
DR InterPro; IPR006911; ARM-rpt_dom.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR000225; Armadillo.
DR InterPro; IPR045210; RING-Ubox_PUB.
DR InterPro; IPR003613; Ubox_domain.
DR InterPro; IPR013083; Znf_RING/FYVE/PHD.
DR Pfam; PF04826; Arm_2; 1.
DR Pfam; PF04564; U-box; 1.
DR SMART; SM00185; ARM; 4.
DR SMART; SM00504; Ubox; 1.
DR SUPFAM; SSF48371; SSF48371; 1.
DR PROSITE; PS50176; ARM_REPEAT; 1.
DR PROSITE; PS51698; U_BOX; 1.
PE 2: Evidence at transcript level;
KW Reference proteome; Repeat; Transferase; Ubl conjugation pathway.
FT CHAIN 1..771
FT /note="U-box domain-containing protein 6"
FT /id="PRO_0000322151"
FT DOMAIN 274..348
FT /note="U-box"
FT REPEAT 456..499
FT /note="ARM 1"
FT REPEAT 502..542
FT /note="ARM 2"
FT REPEAT 544..581
FT /note="ARM 3"
FT REPEAT 583..622
FT /note="ARM 4"
FT REPEAT 625..664
FT /note="ARM 5"
FT REGION 394..415
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 706..751
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 399..414
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 771 AA; 85319 MW; E5196E4A31B672F5 CRC64;
MDVSELEENL FAASDAKLHG DMCKELSAVY CKVLSIFPSL EEARPRSKSG IQTLCSLHIA
LEKAKNILQH CSECSKLYLA ITGDAVLLKF EKAKSALIDS LRRVEDIVPS SIGSQILDIV
GELEHTKFLL DPSEKEVGDR IIALLQQGKK FDNGSDSTEL EIFHQAATRL SITSSRSALA
ERRALKKVID RARVEEDKRK ESIVAYLLHL MRKYSKLFRS EMMDENDSPC STPCSPTGQG
PNEDRVNAFG RQLSKFGSIN YKPMNSRKSG QMPIPPEELR CPISLQLMYD PVIIASGQTY
ERVCIEKWFS DGHNSCPKTQ QQLPHLSLTP NYCVKGLIAS WCEQNGITVP TGPPESLDLN
YWRLAMSDSE SPNSKSVDSV GLCTPKDIRV VPLEESSTIE SERQQKEKNN APDEVDSEIN
VLEGYQDILA IVDKEEDLAK KCKVVENVRI LLKDNEEARI LMGANGFVEA FLQFLESAVH
DNNAAAQETG AMALFNLAVN NNRNKELMLT SGVIPLLEKM ISCSQSQGPA TALYLNLSCL
EKAKPVIGSS QAVSFFVNLL LQDTKTQCKL DALHALYNLS TYSPNIPTLL SSNIIKSLQV
LASTGNHLWI EKSLAVLLNL ASSREGKEEM ITTQGMISTL ATVLDTGDTV EQEQAVSCLV
ILCTGSESCI QMVLQEGVIP SLVSISVNGS PRGRDKSQKL LMLFREQRHR DQPSPNKEEA
PRKTVSAPMA IPAPVSAPES EVKPLTKSIS RRKTMTRPFS FLWKKSHSIH H
