ID   PUB73_ORYSJ             Reviewed;         586 AA.
AC   Q6K762; A0A0P0VJ54;
DT   05-OCT-2010, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   03-AUG-2022, entry version 100.
DE   RecName: Full=U-box domain-containing protein 73;
DE            EC=2.3.2.27;
DE   AltName: Full=Plant U-box protein 73;
DE            Short=OsPUB73;
DE   AltName: Full=RING-type E3 ubiquitin transferase PUB73 {ECO:0000305};
GN   Name=PUB73; OrderedLocusNames=Os02g0490000;
GN   ORFNames=OsJ_06787, OSJNBa0048K16.15;
OS   Oryza sativa subsp. japonica (Rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX   NCBI_TaxID=39947;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16100779; DOI=10.1038/nature03895;
RG   International rice genome sequencing project (IRGSP);
RT   "The map-based sequence of the rice genome.";
RL   Nature 436:793-800(2005).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=18089549; DOI=10.1093/nar/gkm978;
RG   The rice annotation project (RAP);
RT   "The rice annotation project database (RAP-DB): 2008 update.";
RL   Nucleic Acids Res. 36:D1028-D1033(2008).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA   Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA   Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA   Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA   Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT   "Improvement of the Oryza sativa Nipponbare reference genome using next
RT   generation sequence and optical map data.";
RL   Rice 6:4-4(2013).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA   Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA   Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA   Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA   Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA   Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA   Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA   Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA   Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA   Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA   Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA   Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA   Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA   Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA   McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT   "The genomes of Oryza sativa: a history of duplications.";
RL   PLoS Biol. 3:266-281(2005).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=12869764; DOI=10.1126/science.1081288;
RG   The rice full-length cDNA consortium;
RT   "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT   japonica rice.";
RL   Science 301:376-379(2003).
RN   [6]
RP   FUNCTION, GENE FAMILY, AND NOMENCLATURE.
RX   PubMed=19825583; DOI=10.1093/mp/ssn044;
RA   Zeng L.R., Park C.H., Venu R.C., Gough J., Wang G.L.;
RT   "Classification, expression pattern, and E3 ligase activity assay of rice
RT   U-box-containing proteins.";
RL   Mol. Plant 1:800-815(2008).
CC   -!- FUNCTION: Possesses E3 ubiquitin-protein ligase in vitro.
CC       {ECO:0000269|PubMed:19825583}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine +
CC         [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC         cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.;
CC         EC=2.3.2.27;
CC   -!- PATHWAY: Protein modification; protein ubiquitination.
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DR   EMBL; AP004864; BAD21885.1; -; Genomic_DNA.
DR   EMBL; AP008208; BAF08783.1; -; Genomic_DNA.
DR   EMBL; AP014958; BAS78736.1; -; Genomic_DNA.
DR   EMBL; CM000139; EEE57015.1; -; Genomic_DNA.
DR   EMBL; AK106789; -; NOT_ANNOTATED_CDS; mRNA.
DR   RefSeq; XP_015624182.1; XM_015768696.1.
DR   AlphaFoldDB; Q6K762; -.
DR   SMR; Q6K762; -.
DR   STRING; 4530.OS02T0490000-01; -.
DR   PaxDb; Q6K762; -.
DR   PRIDE; Q6K762; -.
DR   EnsemblPlants; Os02t0490000-01; Os02t0490000-01; Os02g0490000.
DR   GeneID; 4329384; -.
DR   Gramene; Os02t0490000-01; Os02t0490000-01; Os02g0490000.
DR   KEGG; osa:4329384; -.
DR   eggNOG; KOG0167; Eukaryota.
DR   HOGENOM; CLU_032955_0_0_1; -.
DR   InParanoid; Q6K762; -.
DR   OMA; QPARFHD; -.
DR   OrthoDB; 1212091at2759; -.
DR   UniPathway; UPA00143; -.
DR   Proteomes; UP000000763; Chromosome 2.
DR   Proteomes; UP000007752; Chromosome 2.
DR   Proteomes; UP000059680; Chromosome 2.
DR   Genevisible; Q6K762; OS.
DR   GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR   GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR   GO; GO:0004842; F:ubiquitin-protein transferase activity; IDA:UniProtKB.
DR   GO; GO:0016567; P:protein ubiquitination; IDA:UniProtKB.
DR   Gene3D; 1.25.10.10; -; 1.
DR   Gene3D; 3.30.40.10; -; 1.
DR   InterPro; IPR011989; ARM-like.
DR   InterPro; IPR016024; ARM-type_fold.
DR   InterPro; IPR003613; Ubox_domain.
DR   InterPro; IPR013083; Znf_RING/FYVE/PHD.
DR   Pfam; PF04564; U-box; 1.
DR   SUPFAM; SSF48371; SSF48371; 1.
DR   PROSITE; PS51698; U_BOX; 1.
PE   2: Evidence at transcript level;
KW   Reference proteome; Transferase; Ubl conjugation pathway.
FT   CHAIN           1..586
FT                   /note="U-box domain-containing protein 73"
FT                   /id="PRO_0000397693"
FT   DOMAIN          182..258
FT                   /note="U-box"
FT   REGION          21..122
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        46..70
FT                   /note="Pro residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   CONFLICT        453
FT                   /note="V -> A (in Ref. 5; AK106789)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   586 AA;  64510 MW;  C7C2075BB9DE297A CRC64;
     MDPEAEEAQL RLEMELAKKA KADMSGLQRS SSLGLDHAGL YPLPLPPGWR SAPTSPLRTP
     SSPPPLQFPP AWAADVAGTS GSAAPEDDGP ARNAGADEAT AGSAPKNEDP ARAAGADDGP
     TRSDYAAMMR MALAKFQDDD AAADDEEAAS AVMEQAMTGL MDLTYRKAKP PELPYEFATR
     WPIPIAHDGT LQAEVMRDPV ILPSGYSVDQ TYQNNQKRQN PWTNTSTFTD HSLPYSLSVP
     NHLLRDMISA WCLDHSDLSP STTSDTPSTP LEPSEEEQIQ RILKLFSGNS ASQREALKLI
     QLLTKTTKGV QPCLAKYADI IPVLINLRRK YKSSWTQDLE EERLTIILNL TMHRQNREIL
     AGQNELAGAI KKIVKKAGNR GKRTSSLAKV ASIVAVLSEF DMFRKRMLDA GGMKMLRGML
     KIKDTEVITE AATAILALYA DGEGEQPARF HEVPQMLLEC HMFTDGILLL LDRLPKSPRV
     FRKICDQALQ LVNIVMAEDA SGPVTRKGIL SAISLIYEIV ERDVGKMNAV KNMEDFIERL
     RQLSSDRLPM QKMLQVERII RTLSDAFPAP TVRGRCQEPS GSRLLA